AB2346

Anti-NQO1 antibody

Name: Anti-NQO1 antibody (ab2346) | Abcam
Brand: Abcam Limited
SKU: AB2346
Price: 430 USD
Availability: InStock

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(117 Publications)

Goat Polyclonal NQO1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human, Pig samples. Cited in 117 publications. Immunogen corresponding to Synthetic Peptide within Human NQO1 aa 250 to C-terminus.

View Alternative Names

DIA4, NMOR1, NQO1, NAD(P)H dehydrogenase [quinone] 1, Azoreductase, DT-diaphorase, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, DTD, QR1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (AB2346)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (AB2346)

ab2346 (2µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (AB2346)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (AB2346)

ab2346 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Supplier Data

Western blot - Anti-NQO1 antibody (AB2346)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-NQO1 antibody (ab2346) at 0.3 µg/mL

Lane 1:

Human Kidney lysate in RIPA buffer at 35 µg

Lane 2:

Human Lung lysate in RIPA buffer at 35 µg

Lane 3:

Human Duodenum lysate in RIPA buffer, at 35 µg

Predicted band size: 31 kDa

Observed band size: 30 kDa

false

Supplier Data

Western blot - Anti-NQO1 antibody (AB2346)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-NQO1 antibody (ab2346) at 0.3 µg/mL

Lane 1:

U251 cell lysate in RIPA buffer at 35 µg

Lane 2:

HepG2 cell lysate in RIPA buffer at 35 µg

false

Supplier Data

Western blot - Anti-NQO1 antibody (AB2346)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-NQO1 antibody (ab2346) at 0.3 µg/mL

Lane 1:

Rat Kidney lysate in RIPA buffer at 35 µg

Lane 2:

Rat Lung lysate in RIPA buffer at 35 µg

Lane 3:

Rat Duodenum lysate in RIPA buffer at 35 µg

Predicted band size: 31 kDa

Observed band size: 30-32 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human, Pig

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human NQO1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P15559

Specificity

This products is expected to recognize all three reported isoforms (NP_000894.1; NP_001020604.1; NP_001020605.1)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "Primary incubation for 1 hour at room temperature.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "Primary incubation for 1 hour at room temperature.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Principal Names – NQO1; NAD(P)H dehydrogenase, quinone 1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI; diaphorase-4; diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); NAD(P)H:menadione oxidoreductase 1, dioxin-inducible 1. Official Gene Symbol - NQO1. GenBank Accession Number – NP_000894. LocusLink ID - 1728. Gene Ontology terms - cytoplasm; xenobiotic metabolism; detoxification response; cytochrome b5 reductase; nitric oxide biosynthesis; NAD(P)H dehydrogenase (quinone); synaptic transmission, cholinergic.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NQO1 also known as NAD(P)H:quinone oxidoreductase 1 is a cytosolic enzyme involved in two-electron reduction processes. This protein plays a role in detoxification transforming quinones into less reactive and harmful hydroquinones. NQO1 has a molecular weight of approximately 31 kDa and is present in various tissues with high expression in the liver and lungs. Sometimes called DT-diaphorase it contributes to antioxidant protection within cells and assists in the stabilization of other proteins such as p53.

Biological function summary

NQO1 serves a protective function against oxidative stress by reducing quinones and preventing redox cycling that generates reactive oxygen species. Part of a critical network its function integrates with the cellular defense mechanism assisting in maintaining cellular homeostasis. NQO1 helps metabolize xenobiotics and is associated with phase II detoxification working alongside enzymes like glutathione S-transferases but is not part of a complex.

Pathways

NQO1 is an important component of the antioxidant defense pathway participating in the direct enzymatic reduction of quinones protecting cells from oxidative damage. It also interfaces with the KEAP1-NRF2 pathway where the NQO1 gene is regulated by the NRF2 transcription factor that upregulates its expression in response to oxidative stress. NQO1 interlinks with proteins such as p53 through pathways related to apoptotic regulation and cellular stress responses.

Mutations or altered expression of NQO1 have correlations with cancer development and progression notably in liver and lung cancers. This protein's stability influences cancer cell survival particularly under oxidative stress or chemotherapeutic treatments. NQO1 also exhibits links to Alzheimer's disease where its potential role in neuroprotection against oxidative damage draws investigation relating it to proteins like tau and amyloid-beta.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species (By similarity) (PubMed : 8999809, PubMed : 9271353). Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion (PubMed : 15102952, PubMed : 8999809, PubMed : 9271353). Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential (PubMed : 8999809). Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome (PubMed : 15687255, PubMed : 28291250).

See full target information NQO1

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Publications (117)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113519 PubMed41035689

2025

NRF2 activation in cancer cells suppresses immune infiltration into the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Huaichun Wen,Takafumi Suzuki,Anqi Zhang,Miu Sato,Mahiro Matsumoto,Yuka Takahashi,Yushi Takahashi,Masayuki Yamamoto

Experimental animals 74:335-347 PubMed39864840

2025

Intestinal epithelial cell-specific restoration of Nrf2 gene in whole-body-knockout mice ameliorates acute colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Tatsuhiro Sato,Keii To,Fumika Sakurai,Kanako Chihara,Eiji Warabi,Tomonori Isobe,Hideo Suzuki,Junichi Shoda,Kosuke Okada

Redox biology 77:103355 PubMed39307045

2024

Sensor systems of KEAP1 uniquely detecting oxidative and electrophilic stresses separately In vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Miu Sato,Nahoko Yaguchi,Takuya Iijima,Aki Muramatsu,Liam Baird,Takafumi Suzuki,Masayuki Yamamoto

Animal bioscience 38:303-315 PubMed39210798

2024

shRNA-interfered of Nrf2 reveals a critical role for Keap1-Nrf2 signaling pathway during effects of zearalenone induced oxidative stress in IPEC-J2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Cheng,Shu Zhen Jiang,Li Bo Huang,Wei Ren Yang

Redox biology 69:103027 PubMed38184999

2024

Specific targeting of the NRF2/β-TrCP axis promotes beneficial effects in NASH.

Applications

Unspecified application

Species

Unspecified reactive species

Raquel Fernández-Ginés,José Antonio Encinar,Maribel Escoll,Daniel Carnicero-Senabre,José Jiménez-Villegas,Ángel J García-Yagüe,Águeda González-Rodríguez,Irma Garcia-Martinez,Ángela M Valverde,Ana I Rojo,Antonio Cuadrado

Redox biology 69:102986 PubMed38091879

2023

Estrogen genotoxicity causes preferential development of Fuchs endothelial corneal dystrophy in females.

Applications

Unspecified application

Species

Unspecified reactive species

Varun Kumar,Neha Deshpande,Mohit Parekh,Raymond Wong,Shazia Ashraf,Muhammad Zahid,Hanna Hui,Annie Miall,Sylvie Kimpton,Marianne O Price,Francis W Price,Frank J Gonzalez,Eleanor Rogan,Ula V Jurkunas

PloS one 18:e0291880 PubMed37862331

2023

Macrophage specific restoration of the Nrf2 gene in whole-body knockout mice ameliorates steatohepatitis induced by lipopolysaccharide from Porphyromonas gingivalis through enhanced hepatic clearance.

Applications

Unspecified application

Species

Unspecified reactive species

Kanako Chihara,Kosuke Okada,Fumihiko Uchida,Ikuru Miura,Shoichi Komine,Eiji Warabi,Takako Takayama,Hideo Suzuki,Takashi Matsuzaka,Naomi Ishibashi-Kanno,Kenji Yamagata,Toru Yanagawa,Hiroki Bukawa,Junichi Shoda

Nature communications 14:3372 PubMed37291151

2023

Astrocyte-oligodendrocyte interaction regulates central nervous system regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Molina-Gonzalez,Rebecca K Holloway,Zoeb Jiwaji,Owen Dando,Sarah A Kent,Katie Emelianova,Amy F Lloyd,Lindsey H Forbes,Ayisha Mahmood,Thomas Skripuletz,Viktoria Gudi,James A Febery,Jeffrey A Johnson,Jill H Fowler,Tanja Kuhlmann,Anna Williams,Siddharthan Chandran,Martin Stangel,Andrew J M Howden,Giles E Hardingham,Veronique E Miron

The Journal of neuroscience : the official journal of the Society for Neuroscience 43:1051-1071 PubMed36596700

2023

α-Synuclein Pathology and Reduced Neurogenesis in the Olfactory System Affect Olfaction in a Mouse Model of Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Eduardo Martin-Lopez,D J Vidyadhara,Teresa Liberia,Sarah J Meller,Leah E Harmon,Ryan M Hsu,Natalie Spence,Bowen Brennan,Kimberly Han,Betül Yücel,Sreeganga S Chandra,Charles A Greer

Cells 11: PubMed36497181

2022

Micro-RNAs Shuttled by Extracellular Vesicles Secreted from Mesenchymal Stem Cells Dampen Astrocyte Pathological Activation and Support Neuroprotection in In-Vitro Models of ALS.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Provenzano,Sophie Nyberg,Debora Giunti,Carola Torazza,Benedetta Parodi,Tiziana Bonifacino,Cesare Usai,Nicole Kerlero de Rosbo,Marco Milanese,Antonio Uccelli,Pamela J Shaw,Laura Ferraiuolo,Giambattista Bonanno

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NQO1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (AB2346)

Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (AB2346)

Western blot - Anti-NQO1 antibody (AB2346)

Western blot - Anti-NQO1 antibody (AB2346)

Western blot - Anti-NQO1 antibody (AB2346)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (117)

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