AB97385

Anti-NQO1 antibody

Name: Anti-NQO1 antibody (ab97385) | Abcam
Brand: Abcam Limited
SKU: AB97385
Rating: 5 (2 reviews)

(2 Reviews)

(5 Publications)

Rabbit Polyclonal NQO1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human NQO1 aa 200 to C-terminus.

View Alternative Names

DIA4, NMOR1, NQO1, NAD(P)H dehydrogenase [quinone] 1, Azoreductase, DT-diaphorase, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, DTD, QR1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (AB97385)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (AB97385)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab97385 at 1/200 dilution. Lower image merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (AB97385)

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (AB97385)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian endometriosis tissue, staining NQO1 with ab97385.

Antigen retrieval was performed in sodium citrate buffer in a microwave oven. Samples were blocked with rabbit serum for one hour before incubating overnight with primary antibody (1/200). Stainined was detected using DAB.

Image from Hevir N et al., Molecular and Cellular Endocrinology, Volume 367, Issue1-2, March 2013, Pages 74-84. Fig 4.; doi: 10.1016/j.mce.2012.12.019. Epub 2012 Dec 28 with permission from Elsevier.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (AB97385)

Immunohistochemical analysis of paraffin-embedded human rectal carcinoma tissue stained for NQO1 protein at cytoplasm with ab97385 at a 1/500 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (AB97385)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue stained for NQO1 protein at cytoplasm with ab97385 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-NQO1 antibody (AB97385)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-NQO1 antibody (ab97385) at 1/500 dilution

All lanes:

Mouse heart lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human NQO1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P15559

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "In some tested cell lines double bands (~26-34kDa) are seen: 293T, A431, H1299, HeLa, HepG2.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "In some tested cell lines double bands (~26-34kDa) are seen: 293T, A431, H1299, HeLa, HepG2.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "In some tested cell lines double bands (~26-34kDa) are seen: 293T, A431, H1299, HeLa, HepG2.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NQO1 also known as NAD(P)H:quinone oxidoreductase 1 is a cytosolic enzyme involved in two-electron reduction processes. This protein plays a role in detoxification transforming quinones into less reactive and harmful hydroquinones. NQO1 has a molecular weight of approximately 31 kDa and is present in various tissues with high expression in the liver and lungs. Sometimes called DT-diaphorase it contributes to antioxidant protection within cells and assists in the stabilization of other proteins such as p53.

Biological function summary

NQO1 serves a protective function against oxidative stress by reducing quinones and preventing redox cycling that generates reactive oxygen species. Part of a critical network its function integrates with the cellular defense mechanism assisting in maintaining cellular homeostasis. NQO1 helps metabolize xenobiotics and is associated with phase II detoxification working alongside enzymes like glutathione S-transferases but is not part of a complex.

Pathways

NQO1 is an important component of the antioxidant defense pathway participating in the direct enzymatic reduction of quinones protecting cells from oxidative damage. It also interfaces with the KEAP1-NRF2 pathway where the NQO1 gene is regulated by the NRF2 transcription factor that upregulates its expression in response to oxidative stress. NQO1 interlinks with proteins such as p53 through pathways related to apoptotic regulation and cellular stress responses.

Mutations or altered expression of NQO1 have correlations with cancer development and progression notably in liver and lung cancers. This protein's stability influences cancer cell survival particularly under oxidative stress or chemotherapeutic treatments. NQO1 also exhibits links to Alzheimer's disease where its potential role in neuroprotection against oxidative damage draws investigation relating it to proteins like tau and amyloid-beta.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species (By similarity) (PubMed : 8999809, PubMed : 9271353). Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion (PubMed : 15102952, PubMed : 8999809, PubMed : 9271353). Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential (PubMed : 8999809). Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome (PubMed : 15687255, PubMed : 28291250).

See full target information NQO1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules & biomedicine 25:1322-1334 PubMed39601768

2024

PF4 regulates neuronal ferroptosis in cerebral hemorrhage through CXCR3/PI3K/AKT/Nrf2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Na Hu,Yunfeng Li,Guohong Zhang,Wei Wang,Liping An,Ran An,Yu Liu

International journal of molecular medicine 45:1294-1304 PubMed32323734

2020

Melatonin elicits protective effects on OGD/R‑insulted H9c2 cells by activating PGC‑1α/Nrf2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Zhi,Kai Li,Hongbing Wang,Ming Lei,Yingqiang Guo

Drug design, development and therapy 13:2811-2824 PubMed31496657

2019

Piceatannol inhibits oxidative stress through modification of Nrf2-signaling pathway in testes and attenuates spermatogenesis and steroidogenesis in rats exposed to cadmium during adulthood.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxue Shi,Lijie Fu

Biochimica et biophysica acta. Bioenergetics 1859:909-924 PubMed29746824

2018

Reduction of 2-methoxy-1,4-naphtoquinone by mitochondrially-localized Nqo1 yielding NAD supports substrate-level phosphorylation during respiratory inhibition.

Applications

Species

Unspecified reactive species

Dora Ravasz,Gergely Kacso,Viktoria Fodor,Kata Horvath,Vera Adam-Vizi,Christos Chinopoulos

Molecular and cellular endocrinology 367:74-84 PubMed23277161

2012

Disturbed balance between phase I and II metabolizing enzymes in ovarian endometriosis: a source of excessive hydroxy-estrogens and ROS?

Applications

WB, IHC-P

Species

Human, Human

Neli Hevir,Martina Ribič-Pucelj,Tea Lanišnik Rižner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NQO1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody (AB97385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (AB97385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (AB97385)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO1 antibody (AB97385)

Western blot - Anti-NQO1 antibody (AB97385)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise