AB213239

Anti-NQO1 antibody [EPR21230]

Recombinant
KO Validated
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(4 Publications)

Rabbit Recombinant Monoclonal NQO1 antibody. Suitable for WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

DIA4, NMOR1, NQO1, NAD(P)H dehydrogenase [quinone] 1, Azoreductase, DT-diaphorase, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, DTD, QR1

1 Images

Lab

Western blot - Anti-NQO1 antibody [EPR21230] (AB213239)

ab213239 was shown to specifically react with NQO1 in wild type HAP1 cells. No band was observed when NQO1 knockout samples were used. Wild-type and NQO1 knockout samples were subjected to SDS-PAGE. The membrane was then blocked for an hour using 3% milk before being incubated with ab213239 and ab8245 (Mouse anti-GAPDH loading control) overnight at 4°C at a concentration of 1ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NQO1 antibody [EPR21230] (ab213239) at 1 µg/mL

Lane 1:

HAP1 wild-type whole cell lysate at 40 µg

Lane 2:

NQO1 knockout whole cell lysate at 40 µg

Lane 3:

HepG2 whole cell lysate at 20 µg

Lane 4:

MEF1 whole cell lysate at 20 µg

Lane 5:

Human Kidney tissue lysate at 20 µg

Lane 6:

Mouse Kidney tissue lysate at 20 µg

Predicted band size: 31 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21230

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Based on internal western blot testing, this antibody showed no reactivity to NQO1 in mouse samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>Abcam recommends using 3% Milk as a blocking agent.</p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

This product was made using synthetic libraries and phage display technology.

This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

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Properties and storage information

Form

Liquid

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NQO1 also known as NAD(P)H:quinone oxidoreductase 1 is a cytosolic enzyme involved in two-electron reduction processes. This protein plays a role in detoxification transforming quinones into less reactive and harmful hydroquinones. NQO1 has a molecular weight of approximately 31 kDa and is present in various tissues with high expression in the liver and lungs. Sometimes called DT-diaphorase it contributes to antioxidant protection within cells and assists in the stabilization of other proteins such as p53.

Biological function summary

NQO1 serves a protective function against oxidative stress by reducing quinones and preventing redox cycling that generates reactive oxygen species. Part of a critical network its function integrates with the cellular defense mechanism assisting in maintaining cellular homeostasis. NQO1 helps metabolize xenobiotics and is associated with phase II detoxification working alongside enzymes like glutathione S-transferases but is not part of a complex.

Pathways

NQO1 is an important component of the antioxidant defense pathway participating in the direct enzymatic reduction of quinones protecting cells from oxidative damage. It also interfaces with the KEAP1-NRF2 pathway where the NQO1 gene is regulated by the NRF2 transcription factor that upregulates its expression in response to oxidative stress. NQO1 interlinks with proteins such as p53 through pathways related to apoptotic regulation and cellular stress responses.

Mutations or altered expression of NQO1 have correlations with cancer development and progression notably in liver and lung cancers. This protein's stability influences cancer cell survival particularly under oxidative stress or chemotherapeutic treatments. NQO1 also exhibits links to Alzheimer's disease where its potential role in neuroprotection against oxidative damage draws investigation relating it to proteins like tau and amyloid-beta.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species (By similarity) (PubMed : 8999809, PubMed : 9271353). Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion (PubMed : 15102952, PubMed : 8999809, PubMed : 9271353). Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential (PubMed : 8999809). Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome (PubMed : 15687255, PubMed : 28291250).

See full target information NQO1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cellular & molecular biology letters 30:115 PubMed41053540

2025

LncRNA-like MMP14 RNA facilitates colorectal cancer metastasis by suppressing H3K27cr at the STARD13 promoter region.

Applications

Unspecified application

Species

Unspecified reactive species

Mengting Li,Ying Gao,Xiaolin Sun,Wendan Zheng,Mengzhen Zhang,Chenlong Wang,Weiwei Chu,Xuemei Yang,Lin Wang,Maojin Zheng,Wenzhi Xue,Fuxing Dong,Dongsheng Pei,Meijian Liao

Current issues in molecular biology 46:1593-1606 PubMed38392221

2024

Plastoquinone-Derivative SkQ1 Improved the Biliary Intraepithelial Neoplasia during Liver Fluke Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Oxana Zaparina,Anna Kovner,Viktoria Petrova,Nataliya Kolosova,Viatcheslav Mordvinov,Maria Pakharukova

Experimental and therapeutic medicine 23:300 PubMed35340872

2022

Rosiglitazone ameliorates acute hepatic injury via activating the Nrf2 signaling pathway and inhibiting activation of the NLRP3 inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Ma,Ying Ma,Bin-Xi Ma,Ming Ma

RSC advances 9:36366-36373 PubMed35540614

2019

The protective effect of propofol on ionizing radiation-induced hematopoietic system damage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Han,Fengtao Sun,Ying Zhang,Jinyan Wang,Qingguo Liu,Ping Gao,Shubo Zhang

View all publications

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