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Rabbit Recombinant Monoclonal NQO1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat samples.

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Images

Sandwich ELISA - Anti-NQO1 antibody [EPR29321-89] - BSA and Azide free (Detector) (AB323176), expandable thumbnail
  • Sandwich ELISA - Anti-NQO1 antibody [EPR29321-89] - BSA and Azide free (Detector) (AB323176), expandable thumbnail
  • Sandwich ELISA - Anti-NQO1 antibody [EPR29321-89] - BSA and Azide free (Detector) (AB323176), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Mouse
Tested
Rat
Tested

Tested
Tested

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species (PubMed:1703398, PubMed:7862630, PubMed:8999809). Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion (By similarity). Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential (By similarity). Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome (By similarity).

Alternative names

Nmor1, NAD(P)H dehydrogenase [quinone] 1, Azoreductase, DT-diaphorase, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, DTD, QR1

Rabbit Recombinant Monoclonal NQO1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR29321-89
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab323176 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse, Rat NQO1. The recommended pair for sandwich ELISA is:

Capture: Anti-NQO1 antibody [EPR29321-38] - BSA and Azide free (Capture) ab323175, Mouse, Rat NQO1 Capture Antibody (unconjugated)

Detector: ab323176, Mouse, Rat NQO1 Detector Antibody (unconjugated)

The reference range value is 39.06-5000 pg/mL.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Sandwich ELISA - Anti-NQO1 antibody [EPR29321-89] - BSA and Azide free (Detector) (ab323176), expandable thumbnail

    Sandwich ELISA - Anti-NQO1 antibody [EPR29321-89] - BSA and Azide free (Detector) (ab323176)

    Interpolated concentration of native NQO1 was measured at different sample concentrations. Undiluted samples are as follows: mouse plasma 12.5% and rat plasma 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-NQO1 antibody [EPR29321-89] - BSA and Azide free (Detector) (ab323176), expandable thumbnail

    Sandwich ELISA - Anti-NQO1 antibody [EPR29321-89] - BSA and Azide free (Detector) (ab323176)

    Interpolated concentration of native NQO1 was measured at different sample concentrations. Undiluted samples are as follows: mouse stomach tissue extract (40 µg/mL) and rat brain tissue extract (40 µg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

  • Sandwich ELISA - Anti-NQO1 antibody [EPR29321-89] - BSA and Azide free (Detector) (ab323176), expandable thumbnail

    Sandwich ELISA - Anti-NQO1 antibody [EPR29321-89] - BSA and Azide free (Detector) (ab323176)

    Sandwich ELISA of Mouse/Rat NQO1 Antibody Pair - BSA and Azide free ab323174 with the capture antibody Anti-NQO1 antibody [EPR29321-38] - BSA and Azide free (Capture) ab323175 dilution at 2 ug/mL and detector antibody ab323176 dilution at 0.5 ug/mL.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com