Skip to main content

Anti-NQO1 antibody [EPR3309] (ab80588) is a rabbit monoclonal antibody that is used to detect NQO1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with NQO1 knockout cell line validation


Images

Western blot - Anti-NQO1 antibody [EPR3309] (AB80588), expandable thumbnail
  • Western blot - Anti-NQO1 antibody [EPR3309] (AB80588), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-NQO1 antibody [EPR3309] (AB80588), expandable thumbnail
  • Immunoprecipitation - Anti-NQO1 antibody [EPR3309] (AB80588), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody [EPR3309] (AB80588), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/30 - 1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/10000 - 1/150000
Notes

-

Species
Rat
Dilution info
1/10000 - 1/150000
Notes

-

Species
Human
Dilution info
1/10000 - 1/150000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50 - 1/80
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

9 products for Alternative Product

Target data

Function

Flavin-containing quinone reductase that catalyzes two-electron reduction of quinones to hydroquinones using either NADH or NADPH as electron donors. In a ping-pong kinetic mechanism, the electrons are sequentially transferred from NAD(P)H to flavin cofactor and then from reduced flavin to the quinone, bypassing the formation of semiquinone and reactive oxygen species (By similarity) (PubMed:8999809, PubMed:9271353). Regulates cellular redox state primarily through quinone detoxification. Reduces components of plasma membrane redox system such as coenzyme Q and vitamin quinones, producing antioxidant hydroquinone forms. In the process may function as superoxide scavenger to prevent hydroquinone oxidation and facilitate excretion (PubMed:15102952, PubMed:8999809, PubMed:9271353). Alternatively, can activate quinones and their derivatives by generating redox reactive hydroquinones with DNA cross-linking antitumor potential (PubMed:8999809). Acts as a gatekeeper of the core 20S proteasome known to degrade proteins with unstructured regions. Upon oxidative stress, interacts with tumor suppressors TP53 and TP73 in a NADH-dependent way and inhibits their ubiquitin-independent degradation by the 20S proteasome (PubMed:15687255, PubMed:28291250).

Alternative names

DIA4, NMOR1, NQO1, NAD(P)H dehydrogenase [quinone] 1, Azoreductase, DT-diaphorase, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, DTD, QR1

Recommended products

Anti-NQO1 antibody [EPR3309] (ab80588) is a rabbit monoclonal antibody that is used to detect NQO1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, ICC/IF. Suitable for Human, Mouse, Rat samples.



- Specificity confirmed with NQO1 knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3309
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

What is this antibody validated in?


Anti-NQO1 antibody [EPR3309] (ab80588) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), in Human, Mouse, Rat samples.

What is the molecular weight of NQO1?


Anti-NQO1 [EPR3309] (ab80588) specifically detects a band for NQO1 (UniProt: P15559) at a molecular weight of 31kDa.

Recommended positive controls


WB: SH-SY5Y, MCF7, HeLa and A549 cell lysates, rat brain tissue lysate.ICC/IF: MCF-7 cells.IP: HeLa cell lysate.

Trusted by the scientific community


Anti-NQO1 [EPR3309] (ab80588) was first used in a scientific publication in 2009 and has been cited over 130 times in peer-reviewed journals.

Reviewed by scientists


Anti-NQO1 [EPR3309] (ab80588) has over 5 independent reviews from customers.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed


The specificity of Anti-NQO1 antibody [EPR3309] (ab80588) has been confirmed by Western blot testing in NQO1 Knockout HAP1 cells.



Other related products


We have a range of other formats of antibody clone [EPR3309] also available for your convenience:
ab80588, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-NQO1 antibody [EPR3309] ab196196, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-NQO1 antibody [EPR3309] ab196465, Carrier free - Anti-NQO1 antibody [EPR3309] - BSA and Azide free ab239896



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NQO1 also known as NAD(P)H:quinone oxidoreductase 1 is a cytosolic enzyme involved in two-electron reduction processes. This protein plays a role in detoxification transforming quinones into less reactive and harmful hydroquinones. NQO1 has a molecular weight of approximately 31 kDa and is present in various tissues with high expression in the liver and lungs. Sometimes called DT-diaphorase it contributes to antioxidant protection within cells and assists in the stabilization of other proteins such as p53.

Biological function summary

NQO1 serves a protective function against oxidative stress by reducing quinones and preventing redox cycling that generates reactive oxygen species. Part of a critical network its function integrates with the cellular defense mechanism assisting in maintaining cellular homeostasis. NQO1 helps metabolize xenobiotics and is associated with phase II detoxification working alongside enzymes like glutathione S-transferases but is not part of a complex.

Pathways

NQO1 is an important component of the antioxidant defense pathway participating in the direct enzymatic reduction of quinones protecting cells from oxidative damage. It also interfaces with the KEAP1-NRF2 pathway where the NQO1 gene is regulated by the NRF2 transcription factor that upregulates its expression in response to oxidative stress. NQO1 interlinks with proteins such as p53 through pathways related to apoptotic regulation and cellular stress responses.

Associated diseases and disorders

Mutations or altered expression of NQO1 have correlations with cancer development and progression notably in liver and lung cancers. This protein's stability influences cancer cell survival particularly under oxidative stress or chemotherapeutic treatments. NQO1 also exhibits links to Alzheimer's disease where its potential role in neuroprotection against oxidative damage draws investigation relating it to proteins like tau and amyloid-beta.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-NQO1 antibody [EPR3309] (ab80588), expandable thumbnail

    Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)

    Lanes 1 - 6: Merged signal (red and green). Green - ab80588 observed at 31 kDa. Red - loading control, Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484, observed at 37 kDa.

    ab80588 was shown to recognize NQO1 in wild-type HAP1 cells as signal was lost at the expected MW in NQO1 knockout cells. Wild-type and NQO1 knockout samples were subjected to SDS-PAGE. ab80588 and Anti-GAPDH antibody [mAbcam 9484] - Loading Control ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-NQO1 antibody [EPR3309] (ab80588) at 1 µg/mL

    Lane 1: Wild-type HAP1 whole cell lysate at 40 µg

    Lane 2: NQO1 knockout HAP1 whole cell lysate at 40 µg

    Lane 3: HepG2 whole cell lysate at 20 µg

    Lane 4: MEF1 whole cell lysate at 20 µg

    Lane 5: Human Kidney tissue lysate at 20 µg

    Lane 6: Mouse Kidney tissue lysate at 20 µg

    Predicted band size: 31 kDa

  • Western blot - Anti-NQO1 antibody [EPR3309] (ab80588), expandable thumbnail

    Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-NQO1 antibody [EPR3309] (ab80588) at 1/25000 dilution

    Lane 1: SH-SY5Y cell lysate at 20 µg

    Lane 2: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 31 kDa

    Observed band size: 31 kDa

  • Flow Cytometry (Intracellular) - Anti-NQO1 antibody [EPR3309] (ab80588), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-NQO1 antibody [EPR3309] (ab80588)

    ab80588 staining NQO1in the human cell line MCF-7 (human breast carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde, permeabilized with 90% methanol and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluorr® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Immunoprecipitation - Anti-NQO1 antibody [EPR3309] (ab80588), expandable thumbnail

    Immunoprecipitation - Anti-NQO1 antibody [EPR3309] (ab80588)

    ab80588 (purified) at 1/50 immunoprecipitating NQO1 in HeLa cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-NQO1 antibody [EPR3309] (ab80588)

    Predicted band size: 31 kDa

    Observed band size: 31 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody [EPR3309] (ab80588), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NQO1 antibody [EPR3309] (ab80588)

    Immunocytochemsitry/Immunofluorescence analysis of MCF-7 cells labelling NQO1 (green) with purified ab80588 at 1/80. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/200) was used as the secondary antibody. Counterstained with DAPI (blue).

  • Western blot - Anti-NQO1 antibody [EPR3309] (ab80588), expandable thumbnail

    Western blot - Anti-NQO1 antibody [EPR3309] (ab80588)

    All lanes: Western blot - Anti-NQO1 antibody [EPR3309] (ab80588) at 1/50000 dilution

    Lane 1: MCF7 cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: A549 cell lysate at 10 µg

    Secondary

    All lanes: goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 31 kDa

    Observed band size: 31 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com