Rabbit Polyclonal NQO2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NQO2.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human NQO2. Database link P16083
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/150 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
Alternative names
NMOR2, NQO2, Ribosyldihydronicotinamide dehydrogenase [quinone], NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, Quinone reductase 2, QR2
Recommended products
Rabbit Polyclonal NQO2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NQO2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human NQO2. Database link P16083
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NQO2 also known as NRH:quinone oxidoreductase 2 is a flavoprotein enzyme playing roles in cellular redox reactions. It has a molecular mass of approximately 25 kDa. NQO2 is expressed widely in human tissues such as liver colon and brain. It catalyzes the two-electron reduction of quinones to hydroquinones therefore helping prevent oxidative stress by converting reactive quinone species into less reactive and more stable molecules. The protein often interacts with other cellular components to perform its functions effectively.
- Biological function summary
NQO2 participates in detoxification processes and acts as a dihydronicotinamide riboside:quinone oxidoreductase. Although it is not part of a large protein complex NQO2 may associate with other molecules or enzymes that modulate cellular antioxidant defense systems. It serves as a backup system when other primary antioxidant enzymes such as NQO1 are impaired. This function reflects the importance of NQO2 in maintaining cellular health and response to environmental stresses.
- Pathways
NQO2 is involved in metabolism and oxidative stress response pathways. Its role in quinone detoxification connects it to the cellular defense mechanism against oxidative damage interacting with proteins like NQO1. NQO2's enzymatic activity goes hand-in-hand with the NAD(P)H dehydrogenase (quinone) pathway which collaborates with other proteins to maintain redox homeostasis and protect cells against electrophilic and oxidative stress.
- Associated diseases and disorders
Alterations in NQO2 expression or function are linked to cancer and neurodegenerative diseases. NQO2's involvement in detoxifying potent quinones links it to cancerous developments when these processes fail while its role against oxidative stress connects it to neurodegenerative conditions like Parkinson’s disease. During these conditions interactions with proteins such as NQO1 and p53 can be significant. The balance of NQO2 activity may contribute to disease progression or suppression highlighting its therapeutic potential in disease management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO2 antibody (ab236917)
IHC image of ab236917 diluted at 1:150 and staining in paraffin-embedded human breast tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. Secondary antibody was a Goat anti-rabbit polymer IgG labelled with HRP and visualized using 0.05% DAB. Secondary antibody only control: 1% BSA instead of primary antibody.
Western blot - Anti-NQO2 antibody (ab236917)
All lanes: Western blot - Anti-NQO2 antibody (ab236917) at 1/1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: K562 whole cell lysate
Lane 3: HepG2 whole cell lysate
Lane 4: JK whole cell lysate
Lane 5: A549 whole cell lysate
Lane 6: MCF7 whole cell lysate
Lane 7: Ntera2 whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 26 kDa
Observed band size: 26 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO2 antibody (ab236917)
IHC image of ab236917 diluted at 1:150 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. Secondary antibody was a Goat anti-rabbit polymer IgG labelled with HRP and visualized using 0.05% DAB. Secondary antibody only control: 1% BSA instead of primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO2 antibody (ab236917)
IHC image of ab236917 diluted at 1:150 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. Secondary antibody was a Goat anti-rabbit polymer IgG labelled with HRP and visualized using 0.05% DAB. Secondary antibody only control: 1% BSA instead of primary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-NQO2 antibody (ab236917)
(Left) Immunofluorescence staining of Hela (human epithelial cell line from cervix adenocarcinoma) cells with ab236917 at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).
(Right) Negative control: Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).
Immunocytochemistry/ Immunofluorescence - Anti-NQO2 antibody (ab236917)
Immunofluorescence staining of U251 cells with ab236917 at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with ab236917 overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).
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