Anti-NQO2 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO2 antibody (AB236917)

IHC image of ab236917 diluted at 1 : 150 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. Secondary antibody was a Goat anti-rabbit polymer IgG labelled with HRP and visualized using 0.05% DAB. Secondary antibody only control : 1% BSA instead of primary antibody.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NQO2 antibody (AB236917)

Immunofluorescence staining of U251 cells with ab236917 at 1 : 20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with ab236917 overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO2 antibody (AB236917)

IHC image of ab236917 diluted at 1 : 150 and staining in paraffin-embedded human breast tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. Secondary antibody was a Goat anti-rabbit polymer IgG labelled with HRP and visualized using 0.05% DAB. Secondary antibody only control : 1% BSA instead of primary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NQO2 antibody (AB236917)

IHC image of ab236917 diluted at 1 : 150 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. Secondary antibody was a Goat anti-rabbit polymer IgG labelled with HRP and visualized using 0.05% DAB. Secondary antibody only control : 1% BSA instead of primary antibody.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NQO2 antibody (AB236917)

(Left) Immunofluorescence staining of Hela (human epithelial cell line from cervix adenocarcinoma) cells with ab236917 at 1 : 20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).

(Right) Negative control : Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L).

• WB

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Western blot - Anti-NQO2 antibody (AB236917)

All lanes:

Western blot - Anti-NQO2 antibody (ab236917) at 1/1000 dilution

Lane 1:

Hela whole cell lysate

Lane 2:

K562 whole cell lysate

Lane 3:

HepG2 whole cell lysate

Lane 4:

JK whole cell lysate

Lane 5:

A549 whole cell lysate

Lane 6:

MCF7 whole cell lysate

Lane 7:

Ntera2 whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 26 kDa

Observed band size: 26 kDa

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