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AB181049

Anti-NQO2 antibody [EPR13345]

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(3 Publications)

Rabbit Recombinant Monoclonal NQO2 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 3 publications.

View Alternative Names

NMOR2, NQO2, Ribosyldihydronicotinamide dehydrogenase [quinone], NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, Quinone reductase 2, QR2

3 Images
Flow Cytometry (Intracellular) - Anti-NQO2 antibody [EPR13345] (AB181049)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NQO2 antibody [EPR13345] (AB181049)

Intracellular flow cytometric analysis of C6 cells labeling NQO2 with ab181049 at 1/140 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

Western blot - Anti-NQO2 antibody [EPR13345] (AB181049)
  • WB

Supplier Data

Western blot - Anti-NQO2 antibody [EPR13345] (AB181049)

Blocking/ Dilution buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-NQO2 antibody [EPR13345] (ab181049) at 1/5000 dilution

Lane 1:

L6 cell lysate at 10 µg

Lane 2:

C2C12 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

false

Western blot - Anti-NQO2 antibody [EPR13345] (AB181049)
  • WB

Supplier Data

Western blot - Anti-NQO2 antibody [EPR13345] (AB181049)

All lanes:

Western blot - Anti-NQO2 antibody [EPR13345] (ab181049) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Lane 3:

Rat kidney tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

false

  • Carrier free

    Anti-NQO2 antibody [EPR13345] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13345

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Alternative Products

Primary Antibodies

AB236917

Anti-NQO2 antibody

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Primary Antibodies

AB137612

Anti-NQO2 antibody

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Complementary Products

Proteins & Peptides

AB93933

Recombinant Human NQO2 protein

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Primary Antibodies

AB80588

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RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-NQO1 antibody [EPR3309] (AB80588)

  • 4
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Primary Antibodies

AB206129

Anti-PKM antibody [EPR10138(B)] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (AB206129)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/140", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NQO2 also known as NRH:quinone oxidoreductase 2 is a flavoprotein enzyme playing roles in cellular redox reactions. It has a molecular mass of approximately 25 kDa. NQO2 is expressed widely in human tissues such as liver colon and brain. It catalyzes the two-electron reduction of quinones to hydroquinones therefore helping prevent oxidative stress by converting reactive quinone species into less reactive and more stable molecules. The protein often interacts with other cellular components to perform its functions effectively.
Biological function summary

NQO2 participates in detoxification processes and acts as a dihydronicotinamide riboside:quinone oxidoreductase. Although it is not part of a large protein complex NQO2 may associate with other molecules or enzymes that modulate cellular antioxidant defense systems. It serves as a backup system when other primary antioxidant enzymes such as NQO1 are impaired. This function reflects the importance of NQO2 in maintaining cellular health and response to environmental stresses.

Pathways

NQO2 is involved in metabolism and oxidative stress response pathways. Its role in quinone detoxification connects it to the cellular defense mechanism against oxidative damage interacting with proteins like NQO1. NQO2's enzymatic activity goes hand-in-hand with the NAD(P)H dehydrogenase (quinone) pathway which collaborates with other proteins to maintain redox homeostasis and protect cells against electrophilic and oxidative stress.

Alterations in NQO2 expression or function are linked to cancer and neurodegenerative diseases. NQO2's involvement in detoxifying potent quinones links it to cancerous developments when these processes fail while its role against oxidative stress connects it to neurodegenerative conditions like Parkinson’s disease. During these conditions interactions with proteins such as NQO1 and p53 can be significant. The balance of NQO2 activity may contribute to disease progression or suppression highlighting its therapeutic potential in disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
See full target information NQO2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:920 PubMed40817243

2025

MRO/HNRNPU/CCL5 feedback loop amplifies M2 macrophage and breast cancer cell crosstalk to drive progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhirui Zeng,Xin Bao,Shan Lei,Wenjing Tang,Huading Tai,Lu Wang,Dahuan Li,Shu Liu,Tengxiang Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400253 PubMed39119847

2024

Homeostatic Shrinkage of Dendritic Spines Requires Melatonin Type 3 Receptor Activation During Sleep.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyin Li,Xin Li,Minmin Lu,Quanhui Chen,Di Yao,Xiaoqian Yu,Zhen Li,Woo-Ping Ge,Na Wang,Jiehua Jin,Yaling Wang,Yixiang Liao,Fenlan Luo,Jie Yan,Xuedan Chen,Chenggang Jiang,Faguo Yue,Dong Gao,Xiangdong Tang,Hong Guo,Yanjiang Wang,Xiaowei Chen,Jianxia Xia,Min Xu,Shuancheng Ren,Chao He,Zhian Hu

Journal of proteome research 20:2596-2606 PubMed33793246

2021

Reduced Nicotinamide Mononucleotide (NMNH) Potently Enhances NAD and Suppresses Glycolysis, the TCA Cycle, and Cell Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Chengting Luo,Ting Li,Wenhao Zhang,Zhaoyun Zong,Xiaohui Liu,Haiteng Deng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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