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AB250309

Anti-NQO2 antibody [EPR13345] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal NQO2 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

NMOR2, NQO2, Ribosyldihydronicotinamide dehydrogenase [quinone], NRH dehydrogenase [quinone] 2, NRH:quinone oxidoreductase 2, Quinone reductase 2, QR2

3 Images
Flow Cytometry (Intracellular) - Anti-NQO2 antibody [EPR13345] - BSA and Azide free (AB250309)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NQO2 antibody [EPR13345] - BSA and Azide free (AB250309)

This data was developed using ab181049, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of C6 cells labeling NQO2 with ab181049 at 1/140 dilution (red). Goat anti rabbit IgG (FITC) used as the secondary at 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.

Western blot - Anti-NQO2 antibody [EPR13345] - BSA and Azide free (AB250309)
  • WB

Supplier Data

Western blot - Anti-NQO2 antibody [EPR13345] - BSA and Azide free (AB250309)

This data was developed using ab181049, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NQO2 antibody [EPR13345] (<a href='/en-us/products/primary-antibodies/nqo2-antibody-epr13345-ab181049'>ab181049</a>) at 1/5000 dilution

Lane 1:

L6 cell lysate at 10 µg

Lane 2:

C2C12 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

false

Western blot - Anti-NQO2 antibody [EPR13345] - BSA and Azide free (AB250309)
  • WB

Supplier Data

Western blot - Anti-NQO2 antibody [EPR13345] - BSA and Azide free (AB250309)

This data was developed using ab181049, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NQO2 antibody [EPR13345] (<a href='/en-us/products/primary-antibodies/nqo2-antibody-epr13345-ab181049'>ab181049</a>) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Lane 3:

Rat kidney tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13345

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Proteins & Peptides

AB93933

Recombinant Human NQO2 protein

SDS-PAGE - Recombinant Human NQO2 protein (AB93933)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB80588

Anti-NQO1 antibody [EPR3309]

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-NQO1 antibody [EPR3309] (AB80588)

  • 4
  • 5
Primary Antibodies

AB206129

Anti-PKM antibody [EPR10138(B)] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PKM antibody [EPR10138(B)] - BSA and Azide free (AB206129)

  • 5
  • 2
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250309 is the carrier-free version of ab181049.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NQO2 also known as NRH:quinone oxidoreductase 2 is a flavoprotein enzyme playing roles in cellular redox reactions. It has a molecular mass of approximately 25 kDa. NQO2 is expressed widely in human tissues such as liver colon and brain. It catalyzes the two-electron reduction of quinones to hydroquinones therefore helping prevent oxidative stress by converting reactive quinone species into less reactive and more stable molecules. The protein often interacts with other cellular components to perform its functions effectively.
Biological function summary

NQO2 participates in detoxification processes and acts as a dihydronicotinamide riboside:quinone oxidoreductase. Although it is not part of a large protein complex NQO2 may associate with other molecules or enzymes that modulate cellular antioxidant defense systems. It serves as a backup system when other primary antioxidant enzymes such as NQO1 are impaired. This function reflects the importance of NQO2 in maintaining cellular health and response to environmental stresses.

Pathways

NQO2 is involved in metabolism and oxidative stress response pathways. Its role in quinone detoxification connects it to the cellular defense mechanism against oxidative damage interacting with proteins like NQO1. NQO2's enzymatic activity goes hand-in-hand with the NAD(P)H dehydrogenase (quinone) pathway which collaborates with other proteins to maintain redox homeostasis and protect cells against electrophilic and oxidative stress.

Alterations in NQO2 expression or function are linked to cancer and neurodegenerative diseases. NQO2's involvement in detoxifying potent quinones links it to cancerous developments when these processes fail while its role against oxidative stress connects it to neurodegenerative conditions like Parkinson’s disease. During these conditions interactions with proteins such as NQO1 and p53 can be significant. The balance of NQO2 activity may contribute to disease progression or suppression highlighting its therapeutic potential in disease management.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The enzyme apparently serves as a quinone reductase in connection with conjugation reactions of hydroquinones involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis.
See full target information NQO2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Evidence-based complementary and alternative medicine : eCAM 2022:7757833 PubMed36091585

2022

Esculentoside A Alleviates Intestinal Dysmotility in Ulcerative Colitis by Regulating HS/CSE and NO/nNOS Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Wenhua Wei,Shiwei Liang,Haicheng Fang,Jie Cao
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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