Rabbit Recombinant Monoclonal NR0B1 / Dax1 antibody. N-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (AB196649), expandable thumbnail](https://content.abcam.com/products/images/nr0b1-dax1-antibody-ep13786-n-terminal-ab196649--immunocytochemistry-immunofluorescence-img67489.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (AB196649)")
![Flow Cytometry (Intracellular) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (AB196649), expandable thumbnail](https://content.abcam.com/products/images/nr0b1-dax1-antibody-ep13786-n-terminal-ab196649--flow-cytometry-intracellular-img38566.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (AB196649)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (AB196649), expandable thumbnail](https://content.abcam.com/products/images/nr0b1-dax1-antibody-ep13786-n-terminal-ab196649--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56167.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (AB196649)")
![Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (AB196649), expandable thumbnail](https://content.abcam.com/products/images/nr0b1-dax1-antibody-ep13786-n-terminal-ab196649--western-blot-img146270.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (AB196649)")
![Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (AB196649), expandable thumbnail](https://content.abcam.com/products/images/nr0b1-dax1-antibody-ep13786-n-terminal-ab196649--western-blot-img56124.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (AB196649)")
Publications
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- Neuropathology and applied neurobiology 47:415-4272020Gonadotroph tumours with a low SF-1 labelling index are more likely to recur and are associated with enrichment of the PI3K-AKT pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRichard A Hickman,Jeffrey N Bruce,Marc Otten,Alexander G Khandji,Xena E Flowers,Markus Siegelin,Beatriz Lopes,Phyllis L Faust,Pamela U FredaPubMed 33128255
- Molecular cancer therapeutics 19:1183-11962020CDK9 Blockade Exploits Context-dependent Transcriptional Changes to Improve Activity and Limit Toxicity of Mithramycin for Ewing Sarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuillermo Flores et. al.PubMed 32127464
- Frontiers in pharmacology 10:2142019A Model of Glucocorticoid Receptor Interaction With Coregulators Predicts Transcriptional Regulation of Target Genes.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/300 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Orphan nuclear receptor. Component of a cascade required for the development of the hypothalamic-pituitary-adrenal-gonadal axis. Acts as a coregulatory protein that inhibits the transcriptional activity of other nuclear receptors through heterodimeric interactions. May also have a role in the development of the embryo and in the maintenance of embryonic stem cell pluripotency (By similarity).
Alternative names
AHC, DAX1, NR0B1, Nuclear receptor subfamily 0 group B member 1, DSS-AHC critical region on the X chromosome protein 1, Nuclear receptor DAX-1
Recommended products
Rabbit Recombinant Monoclonal NR0B1 / Dax1 antibody. N-terminal. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP13786
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NR0B1 also known as Dax1 is a nuclear receptor protein that acts as a transcriptional regulator. It has an approximate mass of 51 kDa and plays an important role in modulating gene expression. This protein is highly expressed in tissues such as the adrenal gland hypothalamus testis and ovary where it exerts its regulatory functions. Although NR0B1 lacks a classical DNA-binding domain it interacts with other proteins to conduct its mechanical functions.
- Biological function summary
NR0B1 interacts with other nuclear receptors and transcription factors to regulate steroidogenesis and reproductive development. It plays a critical role in maintaining the balance of transcriptional activity within these systems. NR0B1 functions as a corepressor and participates in protein complexes modulating the activity of specific gene targets. Its biological activities ensure proper development and differentiation of sex-specific organs.
- Pathways
NR0B1 is actively involved in the steroid hormone biosynthesis and Wnt signaling pathways. In these pathways NR0B1 influences various downstream processes by interacting with proteins such as SF1 and Wnt components. NR0B1 helps coordinate the activities of other transcriptional regulators effectively integrating signals within the pathway to control cellular outcomes.
- Associated diseases and disorders
NR0B1 mutations or dysfunctions can cause conditions such as congenital adrenal hypoplasia and hypogonadotropic hypogonadism. Altered NR0B1 activity interferes with normal adrenal gland and reproductive system development. Its interaction with proteins like SF1 further connects it to pathologies in these systems. Understanding NR0B1's role in these diseases can help develop targeted therapies to address the underlying genetic or functional disruptions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunocytochemistry/ Immunofluorescence - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649), expandable thumbnail](https://content.abcam.com/products/images/nr0b1-dax1-antibody-ep13786-n-terminal-ab196649--immunocytochemistry-immunofluorescence-img67489.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)")
Immunocytochemistry/ Immunofluorescence - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
Immunofluorescent analysis of SH-SY5Y cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling NR0B1 / Dax1 with ab196649 at 1/300 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).
Note: Nuclear and cytoplasm staining on SH-SY5Y cell line was observed.
![Flow Cytometry (Intracellular) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649), expandable thumbnail](https://content.abcam.com/products/images/nr0b1-dax1-antibody-ep13786-n-terminal-ab196649--flow-cytometry-intracellular-img38566.jpg "Flow Cytometry (Intracellular) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)")
Flow Cytometry (Intracellular) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling NR0B1 / Dax1 with purified ab196649 at 1/150 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649), expandable thumbnail](https://content.abcam.com/products/images/nr0b1-dax1-antibody-ep13786-n-terminal-ab196649--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56167.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NR0B1 / Dax1 with ab196649 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Nuclear staining on human testis tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649), expandable thumbnail](https://content.abcam.com/products/images/nr0b1-dax1-antibody-ep13786-n-terminal-ab196649--western-blot-img146270.jpg "Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)")
Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
All lanes: Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649) at 1/1000 dilution
All lanes: Human testis lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 52 kDa
![Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649), expandable thumbnail](https://content.abcam.com/products/images/nr0b1-dax1-antibody-ep13786-n-terminal-ab196649--western-blot-img56124.jpg "Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)")
Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649)
All lanes: Western blot - Anti-NR0B1 / Dax1 antibody [EP13786] - N-terminal (ab196649) at 1/1000 dilution
All lanes: A431 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 52 kDa
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