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AB232841

Anti-NR0B2 antibody

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(3 Publications)

Rabbit Polyclonal NR0B2 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NR0B2.

View Alternative Names

SHP, NR0B2, Nuclear receptor subfamily 0 group B member 2, Orphan nuclear receptor SHP, Small heterodimer partner

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR0B2 antibody (AB232841)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR0B2 antibody (AB232841)

Formalin-fixed, paraffin-embedded human liver tissue stained for NR0B2 using ab232841 at 20 μg/mL in immunohistochemical analysis. DAB staining.

Western blot - Anti-NR0B2 antibody (AB232841)
  • WB

Supplier Data

Western blot - Anti-NR0B2 antibody (AB232841)

All lanes:

Western blot - Anti-NR0B2 antibody (ab232841) at 1 µg/mL

All lanes:

Pig kidney tissue lysate.

Predicted band size: 28 kDa

false

Western blot - Anti-NR0B2 antibody (AB232841)
  • WB

Supplier Data

Western blot - Anti-NR0B2 antibody (AB232841)

All lanes:

Western blot - Anti-NR0B2 antibody (ab232841) at 2 µg/mL

All lanes:

Recombinant human NR0B2 protein

Predicted band size: 28 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NR0B2.

Q15466

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NR0B2 also known as small heterodimer partner (SHP) is a nuclear receptor that functions as a transcriptional regulator. It lacks a conventional DNA-binding domain distinguishing it mechanistically from other nuclear receptors. SHP weighs approximately 28 kDa. It is broadly expressed within the liver pancreas spleen and adrenal gland. In these tissues NR0B2 acts by interacting with other transcription factors to repress the transcription of specific target genes influencing various metabolic processes.
Biological function summary

NR0B2 modulates diverse processes such as bile acid synthesis glucose homeostasis and lipid metabolism. It operates alone and does not form part of a complex. Instead it directly interacts with other nuclear receptors and transcription factors including liver receptor homolog-1 (LRH-1) and retinoid X receptor (RXR) to control gene expression linked to metabolism. Through these interactions SHP acts as a vital checkpoint to maintain metabolic stability within the body.

Pathways

NR0B2 is an important regulator in bile acid and gluconeogenesis pathways. Through its interaction with LRH-1 NR0B2 negatively regulates the bile acid synthesis pathway reducing bile acid production when levels are already sufficient. In terms of gluconeogenesis NR0B2 works alongside forkhead box protein O1 (FoxO1) to manage glucose levels in the liver. This control is critical for energy homeostasis responding to the body's nutritional states.

NR0B2 involvement links to metabolic syndromes and non-alcoholic fatty liver disease (NAFLD). Higher or lower levels of NR0B2 influence the development of these conditions by affecting cholesterol and triglyceride storage. SHP's interactions with proteins like peroxisome proliferator-activated receptor gamma (PPARγ) are significant as these can further disrupt lipid metabolism and enhance the risk or severity of these diseases. Understanding NR0B2's role offers pathways for therapeutic interventions focusing on these metabolic disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator that acts as a negative regulator of receptor-dependent signaling pathways (PubMed : 22504882). Specifically inhibits transactivation of the nuclear receptor with which it interacts (PubMed : 22504882). Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1 (PubMed : 14752053). Essential component of the liver circadian clock which via its interaction with NR1D1 and RORG regulates NPAS2-mediated hepatic lipid metabolism (By similarity). Regulates the circadian expression of cytochrome P450 (CYP) enzymes (By similarity). Represses : NR5A2 and HNF4A to down-regulate CYP2C38, NFLI3 to up-regulate CYP2A5, BHLHE41/HNF1A axis to up-regulate CYP1A2, CYP2E1 and CYP3A11, and NR1D1 to up-regulate CYP2B10, CYP4A10 and CYP4A14 (By similarity).
See full target information NR0B2
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Chinese medicine 19:164 PubMed39578836

2024

Gastrodin attenuates diabetic cardiomyopathy characterized by myocardial fibrosis by inhibiting the KLK8-PAR1 signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

MingShan Zhang,YuFei Zhang,JingGang He,XinRui Wang,YinYin Wang,LinYan Li,Ling Tao,Min Zhang,Xiangchun Shen

International journal of molecular sciences 25: PubMed38542397

2024

Aspirin Caused Intestinal Damage through FXR and ET-1 Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuxia Lin,Binbin Zhang,Manyun Dai,Yan Cheng,Fei Li

Nature communications 14:1829 PubMed37005411

2023

Parabacteroides distasonis ameliorates hepatic fibrosis potentially via modulating intestinal bile acid metabolism and hepatocyte pyroptosis in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhao,Man-Yun Dai,Ruo-Yue Huang,Jing-Yi Duan,Ting Zhang,Wei-Min Bao,Jing-Yi Zhang,Shao-Qiang Gui,Shu-Min Xia,Cong-Ting Dai,Ying-Mei Tang,Frank J Gonzalez,Fei Li
View all publications
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