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AB327185

Anti-NR2C2 Antibody

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Rabbit Polyclonal TR4 antibody. Suitable for WB, IHC-P, ICC/IF, ChIP and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human NR2C2.

View Alternative Names

TAK1, TR4, NR2C2, Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, Testicular receptor 4, Mtr2r1, Tak1, Tr4, Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TAK1, Orphan nuclear receptor TR4, Testicular receptor 4, Tr4, Nuclear receptor subfamily 2 group C member 2, Orphan nuclear receptor TR4, Testicular receptor 4

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2C2 Antibody (AB327185)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2C2 Antibody (AB327185)

Immunohistochemical staining of human testis using ab327185 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2C2 Antibody (AB327185)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2C2 Antibody (AB327185)

Immunohistochemical staining of human cerebral cortex using ab327185 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in neurons.

Immunocytochemistry/ Immunofluorescence - Anti-NR2C2 Antibody (AB327185)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NR2C2 Antibody (AB327185)

Immunofluorescent staining of human cell line A-431 using ab327185 at 0.25–2 µg/ml, showing localization to nucleoplasm.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2C2 Antibody (AB327185)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2C2 Antibody (AB327185)

Immunohistochemical staining of human lymph node using ab327185 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in non-germinal center cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2C2 Antibody (AB327185)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2C2 Antibody (AB327185)

Immunohistochemical staining of human rectum using ab327185 at 1 : 50–1 : 200 dilution, showing strong nuclear positivity in glandular cells.

ChIP - Anti-NR2C2 Antibody (AB327185)
  • ChIP

Supplier Data

ChIP - Anti-NR2C2 Antibody (AB327185)

ChIP-Exo-Seq composite graph for ab327185 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western blot - Anti-NR2C2 Antibody (AB327185)
  • WB

Supplier Data

Western blot - Anti-NR2C2 Antibody (AB327185)

Western blot analysis in human cell line HeLa using ab327185 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-NR2C2 Antibody (ab327185)

All lanes:

Human cell line HeLa

Predicted band size: 65.41 kDa

false

Western blot - Anti-NR2C2 Antibody (AB327185)
  • WB

Supplier Data

Western blot - Anti-NR2C2 Antibody (AB327185)

Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II using ab327185 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-NR2C2 Antibody (ab327185)

Lane 2:

Mouse cell line NIH-3T3

Lane 3:

Rat cell line NBT-II

Predicted band size: 65.41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

ChIP, WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NR2C2.

P49116

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear receptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior (By similarity). Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation.
See full target information NR2C2

Additional targets

,

Product promise

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