Rabbit Polyclonal NR2F6/EAR-2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human NR2F6 aa 300 to C-terminus.
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- Theranostics 14:249-2642024Reprogramming of endothelial gene expression by tamoxifen inhibits angiogenesis and ERα-negative tumor growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChanaëlle Fébrissy,Marine Adlanmerini,Christel Péqueux,Frédéric Boudou,Mélissa Buscato,Adrien Gargaros,Silveric Gilardi-Bresson,Khrystyna Boriak,Henrik Laurell,Coralie Fontaine,Benita S Katzenellenbogen,John A Katzenellenbogen,Julie Guillermet-Guibert,Jean-François Arnal,Raphaël Metivier,Françoise LenfantPubMed 38164151
- Journal of translational medicine 21:2002023Grpel2 maintains cardiomyocyte survival in diabetic cardiomyopathy through DLST-mediated mitochondrial dysfunction: a proof-of-concept study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongjin Yang,Xiaomeng Zhang,Yunyun Zhang,Yingfan Wang,Man Li,Yuancui Meng,Jianbang Wang,Xue Wen,Jun Yu,Pan ChangPubMed 36927450
- Journal of cancer research and clinical oncology 149:7155-71642023Overexpression of the orphan nuclear receptor NR2F6 is associated with improved survival across molecular subgroups in endometrial cancer patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesL Proppe,T Jagomast,S Beume,L Klapper,G Gitas,F Köster,S Perner,A Rody,J Ribbat-Idel,L C HankerPubMed 36884115
- Advanced science (Weinheim, Baden-Wurttemberg, Ger 7:20022732020The Nuclear Orphan Receptor NR2F6 Promotes Hepatic Steatosis through Upregulation of Fatty Acid Transporter CD36.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Zhou et. al.PubMed 33173745
- International journal of molecular sciences 21:2020NR2F6 as a Prognostic Biomarker in HNSCC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLuise Klapper et. al.PubMed 32752295
- Molecular cancer 18:1192019Circular RNA circRHOT1 promotes hepatocellular carcinoma progression by initiation of NR2F6 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiyan Wang,Haiyan Long,Qinghua Zheng,Xiaotong Bo,Xuhua Xiao,Bin LiPubMed 31324186
- Nature communications 9:15382018Nuclear receptor NR2F6 inhibition potentiates responses to PD-L1/PD-1 cancer immune checkpoint blockade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVictoria Klepsch et. al.PubMed 29670099
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NR2F6 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P10588
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes or Tris-EDTA buffer pH8 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Associated Products
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Target data
Function
Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).
Alternative names
EAR2, ERBAL2, NR2F6, Nuclear receptor subfamily 2 group F member 6, V-erbA-related protein 2, EAR-2
Recommended products
Rabbit Polyclonal NR2F6/EAR-2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human NR2F6 aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NR2F6 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P10588
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NR2F6 also known as Ear-2 is an orphan nuclear receptor protein that plays a role as a transcription factor. It often weighs around 46 kDa and is expressed in various tissues including the immune cells and certain cancer cells. As a receptor it regulates gene expression by binding to specific DNA sequences affecting the transcription of genes involved in immune responses and development processes.
- Biological function summary
NR2F6 acts as a negative regulator in Th17 cells and Treg cells suppressing the immune response. It does not form a well-defined complex but interacts with various co-repressors and co-activators to execute its function. Its activity impacts T cell activity and inflammatory responses indicating its role in immune modulation and homeostasis.
- Pathways
NR2F6 participates in the immune signaling pathways and the development of Th17 cells. It influences the IL-17 signaling pathway by interacting with related proteins impacting the secretion of pro-inflammatory cytokines. Furthermore NR2F6 is associated with nuclear receptor pathways interacting with other transcription factors such as RORγt critical for immune cell differentiation.
- Associated diseases and disorders
NR2F6 plays an important role in autoimmune diseases like arthritis and cancer progression. Its ability to modulate immune responses connects it to immune-related disorders where its overexpression may lead to uncontrolled inflammation. Additionally NR2F6 affects the expression of proteins such as PD-1 in cancers where its regulatory functions can alter tumor immunity and potentially affect cancer growth and treatment responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-NR2F6/EAR-2 antibody (ab137496)
10% SDS PAGE
All lanes: Western blot - Anti-NR2F6/EAR-2 antibody (ab137496) at 1/1000 dilution
Lane 1: HeLa whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Predicted band size: 43 kDa
Western blot - Anti-NR2F6/EAR-2 antibody (ab137496)
10% SDS PAGE
All lanes: Western blot - Anti-NR2F6/EAR-2 antibody (ab137496) at 1/500 dilution
Lane 1: NIH 3T3 whole cell lysate at 30 µg
Lane 2: JC whole cell lysate at 30 µg
Predicted band size: 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody (ab137496)
Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue, labelling NR2F6/EAR-2 with ab137496 at 1/100 dilution.
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