AB137496

Anti-NR2F6/EAR-2 antibody

Name: Anti-NR2F6/EAR-2 antibody (ab137496) | Abcam
Brand: Abcam Limited
SKU: AB137496

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(9 Publications)

Rabbit Polyclonal NR2F6/EAR-2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human NR2F6 aa 300 to C-terminus.

View Alternative Names

EAR2, ERBAL2, NR2F6, Nuclear receptor subfamily 2 group F member 6, V-erbA-related protein 2, EAR-2

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody (AB137496)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody (AB137496)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue, labelling NR2F6/EAR-2 with ab137496 at 1/100 dilution.

Supplier Data

Western blot - Anti-NR2F6/EAR-2 antibody (AB137496)

Samples were separated by 10% SDS-PAGE. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-NR2F6/EAR-2 antibody (ab137496) at 1/1000 dilution

All lanes:

Mouse liver tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 43 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human NR2F6 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P10588

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>or Tris-EDTA buffer pH8</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NR2F6 also known as Ear-2 is an orphan nuclear receptor protein that plays a role as a transcription factor. It often weighs around 46 kDa and is expressed in various tissues including the immune cells and certain cancer cells. As a receptor it regulates gene expression by binding to specific DNA sequences affecting the transcription of genes involved in immune responses and development processes.

Biological function summary

NR2F6 acts as a negative regulator in Th17 cells and Treg cells suppressing the immune response. It does not form a well-defined complex but interacts with various co-repressors and co-activators to execute its function. Its activity impacts T cell activity and inflammatory responses indicating its role in immune modulation and homeostasis.

Pathways

NR2F6 participates in the immune signaling pathways and the development of Th17 cells. It influences the IL-17 signaling pathway by interacting with related proteins impacting the secretion of pro-inflammatory cytokines. Furthermore NR2F6 is associated with nuclear receptor pathways interacting with other transcription factors such as RORγt critical for immune cell differentiation.

NR2F6 plays an important role in autoimmune diseases like arthritis and cancer progression. Its ability to modulate immune responses connects it to immune-related disorders where its overexpression may lead to uncontrolled inflammation. Additionally NR2F6 affects the expression of proteins such as PD-1 in cancers where its regulatory functions can alter tumor immunity and potentially affect cancer growth and treatment responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).

See full target information NR2F6

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:21029 PubMed39251671

2024

Characterization of prostate macrophage heterogeneity, foam cell markers, and CXCL17 upregulation in a mouse model of steroid hormone imbalance.

Applications

Unspecified application

Species

Unspecified reactive species

Samara V Silver,Kayah J Tucker,Renee E Vickman,Nadia A Lanman,O John Semmes,Nehemiah S Alvarez,Petra Popovics

Molecular metabolism 81:101891 PubMed38307386

2024

NR2F6 is essential for brown adipocyte differentiation and systemic metabolic homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei-Yu Zhou,Pei Liu,Yi-Fan Xia,Yi-Jie Shi,Hong-Yu Xu,Meng Ding,Qi-Qi Yang,Shu-Wen Qian,Yan Tang,Yan Lu,Qi-Qun Tang,Yang Liu

Theranostics 14:249-264 PubMed38164151

2024

Reprogramming of endothelial gene expression by tamoxifen inhibits angiogenesis and ERα-negative tumor growth.

Applications

Unspecified application

Species

Unspecified reactive species

Chanaëlle Fébrissy,Marine Adlanmerini,Christel Péqueux,Frédéric Boudou,Mélissa Buscato,Adrien Gargaros,Silveric Gilardi-Bresson,Khrystyna Boriak,Henrik Laurell,Coralie Fontaine,Benita S Katzenellenbogen,John A Katzenellenbogen,Julie Guillermet-Guibert,Jean-François Arnal,Raphaël Metivier,Françoise Lenfant

Journal of translational medicine 21:200 PubMed36927450

2023

Grpel2 maintains cardiomyocyte survival in diabetic cardiomyopathy through DLST-mediated mitochondrial dysfunction: a proof-of-concept study.

Applications

Unspecified application

Species

Unspecified reactive species

Rongjin Yang,Xiaomeng Zhang,Yunyun Zhang,Yingfan Wang,Man Li,Yuancui Meng,Jianbang Wang,Xue Wen,Jun Yu,Pan Chang

Journal of cancer research and clinical oncology 149:7155-7164 PubMed36884115

2023

Overexpression of the orphan nuclear receptor NR2F6 is associated with improved survival across molecular subgroups in endometrial cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

L Proppe,T Jagomast,S Beume,L Klapper,G Gitas,F Köster,S Perner,A Rody,J Ribbat-Idel,L C Hanker

Advanced science (Weinheim, Baden-Wurttemberg, Ger 7:2002273 PubMed33173745

2020

The Nuclear Orphan Receptor NR2F6 Promotes Hepatic Steatosis through Upregulation of Fatty Acid Transporter CD36.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Zhou,Lijing Jia,Zhijian Zhang,Liping Xiang,Youwen Yuan,Peilin Zheng,Bin Liu,Xingxing Ren,Hua Bian,Liwei Xie,Yao Li,Jieli Lu,Huijie Zhang,Yan Lu

International journal of molecular sciences 21: PubMed32752295

2020

NR2F6 as a Prognostic Biomarker in HNSCC.

Applications

Unspecified application

Species

Unspecified reactive species

Luise Klapper,Julika Ribbat-Idel,Patrick Kuppler,Finn-Ole Paulsen,Karl-Ludwig Bruchhage,Dirk Rades,Anne Offermann,Jutta Kirfel,Barbara Wollenberg,Christian Idel,Sven Perner

Molecular cancer 18:119 PubMed31324186

2019

Circular RNA circRHOT1 promotes hepatocellular carcinoma progression by initiation of NR2F6 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Liyan Wang,Haiyan Long,Qinghua Zheng,Xiaotong Bo,Xuhua Xiao,Bin Li

Nature communications 9:1538 PubMed29670099

2018

Nuclear receptor NR2F6 inhibition potentiates responses to PD-L1/PD-1 cancer immune checkpoint blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Victoria Klepsch,Natascha Hermann-Kleiter,Patricia Do-Dinh,Bojana Jakic,Anne Offermann,Mirjana Efremova,Sieghart Sopper,Dietmar Rieder,Anne Krogsdam,Gabriele Gamerith,Sven Perner,Alexandar Tzankov,Zlatko Trajanoski,Dominik Wolf,Gottfried Baier

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NR2F6/EAR-2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody (AB137496)

Western blot - Anti-NR2F6/EAR-2 antibody (AB137496)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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