AB65012

Anti-NR2F6/EAR-2 antibody

Name: Anti-NR2F6/EAR-2 antibody (ab65012) | Abcam
Brand: Abcam Limited
SKU: AB65012
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal NR2F6/EAR-2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

EAR2, ERBAL2, NR2F6, Nuclear receptor subfamily 2 group F member 6, V-erbA-related protein 2, EAR-2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-NR2F6/EAR-2 antibody (AB65012)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NR2F6/EAR-2 antibody (AB65012)

ab65012 at 1/500 - 1/1000 dilution staining NR2F6/EAR-2 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody (AB65012)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody (AB65012)

ab65012 at 1/50 - 1/100 dilution staining human cervix carcinoma in NR2F6/EAR-2 by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

Unknown

Western blot - Anti-NR2F6/EAR-2 antibody (AB65012)

All lanes:

Western blot - Anti-NR2F6/EAR-2 antibody (ab65012) at 1/500 dilution

Lane 1:

HeLa cell extract at 10-30µg

Lane 2:

293 cell extract at 10-30µg

Lane 3:

HUVEC cell extract at 10-30µg

Lane 4:

HUVEC cell extract at 10-30µg with immunizing peptide at 5-10µg

Predicted band size: 43 kDa

Observed band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NR2F6 also known as Ear-2 is an orphan nuclear receptor protein that plays a role as a transcription factor. It often weighs around 46 kDa and is expressed in various tissues including the immune cells and certain cancer cells. As a receptor it regulates gene expression by binding to specific DNA sequences affecting the transcription of genes involved in immune responses and development processes.

Biological function summary

NR2F6 acts as a negative regulator in Th17 cells and Treg cells suppressing the immune response. It does not form a well-defined complex but interacts with various co-repressors and co-activators to execute its function. Its activity impacts T cell activity and inflammatory responses indicating its role in immune modulation and homeostasis.

Pathways

NR2F6 participates in the immune signaling pathways and the development of Th17 cells. It influences the IL-17 signaling pathway by interacting with related proteins impacting the secretion of pro-inflammatory cytokines. Furthermore NR2F6 is associated with nuclear receptor pathways interacting with other transcription factors such as RORγt critical for immune cell differentiation.

NR2F6 plays an important role in autoimmune diseases like arthritis and cancer progression. Its ability to modulate immune responses connects it to immune-related disorders where its overexpression may lead to uncontrolled inflammation. Additionally NR2F6 affects the expression of proteins such as PD-1 in cancers where its regulatory functions can alter tumor immunity and potentially affect cancer growth and treatment responses.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor predominantly involved in transcriptional repression. Binds to promoter/enhancer response elements that contain the imperfect 5'-AGGTCA-3' direct or inverted repeats with various spacings which are also recognized by other nuclear hormone receptors. Involved in modulation of hormonal responses. Represses transcriptional activity of the lutropin-choriogonadotropic hormone receptor/LHCGR gene, the renin/REN gene and the oxytocin-neurophysin/OXT gene. Represses the triiodothyronine-dependent and -independent transcriptional activity of the thyroid hormone receptor gene in a cell type-specific manner. The corepressing function towards thyroid hormone receptor beta/THRB involves at least in part the inhibition of THRB binding to triiodothyronine response elements (TREs) by NR2F6. Inhibits NFATC transcription factor DNA binding and subsequently its transcriptional activity. Acts as transcriptional repressor of IL-17 expression in Th-17 differentiated CD4(+) T cells and may be involved in induction and/or maintenance of peripheral immunological tolerance and autoimmunity. Involved in development of forebrain circadian clock; is required early in the development of the locus coeruleus (LC).

See full target information NR2F6

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 20:e1012271 PubMed38829910

2024

The roles of nuclear orphan receptor NR2F6 in anti-viral innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Yang,Chen-Yu Wang,Qiao-Yun Long,Zhuo Cao,Ming-Liang Wei,Shan-Bo Tang,Xiang Lin,Zi-Qi Mu,Yong Xiao,Ming-Kai Chen,Min Wu,Lian-Yun Li

American journal of physiology. Renal physiology 302:F1025-33 PubMed22278040

2012

Regulation of renin expression by the orphan nuclear receptors Nr2f2 and Nr2f6.

Applications

Species

Unspecified reactive species

Eric T Weatherford,Xuebo Liu,Curt D Sigmund

Gastroenterology 138:636-48, 648.e1-12 PubMed19818784

2009

The intestinal nuclear receptor signature with epithelial localization patterns and expression modulation in tumors.

Applications

IHC-P

Species

Human

Salvatore Modica,Francoise Gofflot,Stefania Murzilli,Andria D'Orazio,Lorena Salvatore,Fabio Pellegrini,Antonio Nicolucci,Giovanni Tognoni,Massimiliano Copetti,Rosa Valanzano,Serena Veschi,Renato Mariani-Costantini,Giuseppe Palasciano,Kristina Schoonjans,Johan Auwerx,Antonio Moschetta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NR2F6/EAR-2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NR2F6/EAR-2 antibody (AB65012)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR2F6/EAR-2 antibody (AB65012)

Western blot - Anti-NR2F6/EAR-2 antibody (AB65012)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise