Rabbit Recombinant Monoclonal NR3A antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse samples.
Images
![Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (AB302517), expandable thumbnail](https://content.abcam.com/products/images/nr3a-antibody-epr25287-60-bsa-and-azide-free-ab302517--western-blot-img401152.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (AB302517)")
![Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (AB302517), expandable thumbnail](https://content.abcam.com/products/images/nr3a-antibody-epr25287-60-bsa-and-azide-free-ab302517--western-blot-img401154.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (AB302517)")
![Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (AB302517), expandable thumbnail](https://content.abcam.com/products/images/nr3a-antibody-epr25287-60-bsa-and-azide-free-ab302517--western-blot-img401153.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (AB302517)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | ICC/IF | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Component of a non-conventional N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with low calcium permeability and low voltage-dependent block by Mg(2+). During the development of neural circuits, participates in the synaptic refinement period, restricting spine maturation and growth (By similarity). Forms glutamatergic receptor complexes with GluN1 and GluN2 subunits which are activated by glycine binding to the GluN1 and GluN3 subunits and L-glutamate binding to GluN2 subunits (By similarity). Forms excitatory glycinergic receptor complexes with GluN1 alone which are activated by glycine binding to the GluN1 and GluN3 subunits (By similarity). GluN3A subunit also binds D-serine (By similarity). Each GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (By similarity). By competing with GIT1 interaction with ARHGEF7/beta-PIX, may reduce GIT1/ARHGEF7-regulated local activation of RAC1, hence affecting signaling and limiting the maturation and growth of inactive synapses (PubMed:24297929).
Alternative names
Kiaa1973, Grin3a, GluN3A, N-methyl-D-aspartate receptor, N-methyl-D-aspartate receptor subtype 3A, NMDAR-L, NMDAR3A, NR3A
Recommended products
Rabbit Recombinant Monoclonal NR3A antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25287-60
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The NR3A protein also known as GluN3A is a subunit of the N-methyl-D-aspartate (NMDA) receptor an important glutamate receptor in the brain. It has a molecular weight of about 120 kDa. NR3A is expressed in various regions of the central nervous system but it is mostly present during early brain development. This subunit forms part of a receptor channel complex that is critical for synaptic plasticity and neurotransmission. NR3A regulates ion flow across the neuronal membrane in response to neurotransmitter binding modulating calcium permeability and excitability of neurons.
- Biological function summary
NR3A serves to shape synaptic development and fine-tune signaling during early brain maturation. The NR3A subunit exists in a complex with other NMDA receptor subunits including NR1 and NR2 affecting receptor function by decreasing calcium influx when present. It can influence synaptic pruning and circuit formation which are essential processes in neurodevelopment. The presence of NR3A in the NMDA receptor decreases the receptor's calcium permeability altering the excitatory post-synaptic potential.
- Pathways
Involvement in synaptic plasticity pathways connects NR3A to processes such as long-term potentiation (LTP) and long-term depression (LTD) which are important for learning and memory. The NMDA receptor containing NR3A plays a regulatory role in these pathways by mediating calcium entry into cells affecting downstream signaling events. NR3A has interactions with proteins such as CaMKII and PSD-95 which are key components in signaling cascades linked to synaptic strengthening and synapse stability.
- Associated diseases and disorders
NR3A has associations with neurodevelopmental disorders and neurodegenerative diseases. It is implicated in conditions such as schizophrenia where altered NR3A expression or function may contribute to cognitive deficits. Changes in NR3A levels relate to disruptions in NMDA receptor-mediated neurotransmission and could be linked to abnormal synaptic connectivity. Additionally NR3A might connect to Alzheimer's disease through pathways involving altered NMDA receptor activity where NR1 and NR2 subunits also play critical roles.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (ab302517), expandable thumbnail](https://content.abcam.com/products/images/nr3a-antibody-epr25287-60-bsa-and-azide-free-ab302517--western-blot-img401152.jpg "Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (ab302517)")
Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (ab302517)
This data was developed using Anti-NR3A antibody [EPR25287-60] ab302516, the same antibody clone in a different buffer
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: heart (PMID: 7472412)
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 3 minutesAll lanes: Western blot - Anti-NR3A antibody [EPR25287-60] (Anti-NR3A antibody [EPR25287-60] ab302516) at 1/1000 dilution
Lane 1: Rat thalamus tissue lysate 20 µg
Lane 2: Rat P0 brain tissue lysate 20 µg
Lane 3: Rat heart tissue lysate 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 130 kDa
Exposure time: 3min
![Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (ab302517), expandable thumbnail](https://content.abcam.com/products/images/nr3a-antibody-epr25287-60-bsa-and-azide-free-ab302517--western-blot-img401154.jpg "Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (ab302517)")
Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (ab302517)
This data was developed using Anti-NR3A antibody [EPR25287-60] ab302516, the same antibody clone in a different buffer
Blocking and diluting buffer and concentration: 5% NFDM/TBST
This antibody does not cross-react with rat GluN3B.
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 5.5 secondsAll lanes: Western blot - Anti-NR3A antibody [EPR25287-60] (Anti-NR3A antibody [EPR25287-60] ab302516) at 1/1000 dilution
Lane 1: HEK-293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containi a his tag, whole cell lysate, 20 µg
Lane 2: HEK-293T cells transfected with a rat GluN3A expression vector containi a his tag, whole cell lysate, 20 µg
Lane 3: HEK-293T cells transfected with a rat GluN3B expression vector containi a his tag, whole cell lysate, 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 130 kDa
Exposure time: 5.5s
![Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (ab302517), expandable thumbnail](https://content.abcam.com/products/images/nr3a-antibody-epr25287-60-bsa-and-azide-free-ab302517--western-blot-img401153.jpg "Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (ab302517)")
Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (ab302517)
This data was developed using Anti-NR3A antibody [EPR25287-60] ab302516, the same antibody clone in a different buffer
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates.
Exposure time: 81 secondsAll lanes: Western blot - Anti-NR3A antibody [EPR25287-60] (Anti-NR3A antibody [EPR25287-60] ab302516) at 1/1000 dilution
Lane 1: Mouse thalamus tissue lysate 20 µg
Lane 2: Mouse spinal cord tissue lysate 20 µg
Lane 3: Mouse P1 brain tissue lysate 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 130 kDa
Exposure time: 81s
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