AB302517

Anti-NR3A antibody [EPR25287-60] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal NR3A antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse samples.

View Alternative Names

Kiaa1973, Grin3a, GluN3A, N-methyl-D-aspartate receptor, N-methyl-D-aspartate receptor subtype 3A, NMDAR-L, NMDAR3A, NR3A

3 Images

Supplier Data

Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (AB302517)

This data was developed using ab302516, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST Samples are non-boiled as boiling may cause protein aggregates. Exposure time : 81 seconds

All lanes:

Western blot - Anti-NR3A antibody [EPR25287-60] (<a href='/en-us/products/primary-antibodies/nr3a-antibody-epr25287-60-ab302516'>ab302516</a>) at 1/1000 dilution

Lane 1:

Mouse thalamus tissue lysate 20 µg

Lane 2:

Mouse spinal cord tissue lysate 20 µg

Lane 3:

Mouse P1 brain tissue lysate 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 130 kDa

false

Exposure time: 81s

Supplier Data

Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (AB302517)

This data was developed using ab302516, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with rat GluN3B. Samples are non-boiled as boiling may cause protein aggregates. Exposure time : 5.5 seconds

All lanes:

Western blot - Anti-NR3A antibody [EPR25287-60] (<a href='/en-us/products/primary-antibodies/nr3a-antibody-epr25287-60-ab302516'>ab302516</a>) at 1/1000 dilution

Lane 1:

HEK-293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containi a his tag, whole cell lysate, 20 µg

Lane 2:

HEK-293T cells transfected with a rat GluN3A expression vector containi a his tag, whole cell lysate, 20 µg

Lane 3:

HEK-293T cells transfected with a rat GluN3B expression vector containi a his tag, whole cell lysate, 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 130 kDa

false

Exposure time: 5.5s

Supplier Data

Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (AB302517)

This data was developed using ab302516, the same antibody clone in a different buffer Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : heart (PMID : 7472412) Samples are non-boiled as boiling may cause protein aggregates. Exposure time : 3 minutes

All lanes:

Western blot - Anti-NR3A antibody [EPR25287-60] (<a href='/en-us/products/primary-antibodies/nr3a-antibody-epr25287-60-ab302516'>ab302516</a>) at 1/1000 dilution

Lane 1:

Rat thalamus tissue lysate 20 µg

Lane 2:

Rat P0 brain tissue lysate 20 µg

Lane 3:

Rat heart tissue lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 130 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25287-60

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NR3A protein also known as GluN3A is a subunit of the N-methyl-D-aspartate (NMDA) receptor an important glutamate receptor in the brain. It has a molecular weight of about 120 kDa. NR3A is expressed in various regions of the central nervous system but it is mostly present during early brain development. This subunit forms part of a receptor channel complex that is critical for synaptic plasticity and neurotransmission. NR3A regulates ion flow across the neuronal membrane in response to neurotransmitter binding modulating calcium permeability and excitability of neurons.

Biological function summary

NR3A serves to shape synaptic development and fine-tune signaling during early brain maturation. The NR3A subunit exists in a complex with other NMDA receptor subunits including NR1 and NR2 affecting receptor function by decreasing calcium influx when present. It can influence synaptic pruning and circuit formation which are essential processes in neurodevelopment. The presence of NR3A in the NMDA receptor decreases the receptor's calcium permeability altering the excitatory post-synaptic potential.

Pathways

Involvement in synaptic plasticity pathways connects NR3A to processes such as long-term potentiation (LTP) and long-term depression (LTD) which are important for learning and memory. The NMDA receptor containing NR3A plays a regulatory role in these pathways by mediating calcium entry into cells affecting downstream signaling events. NR3A has interactions with proteins such as CaMKII and PSD-95 which are key components in signaling cascades linked to synaptic strengthening and synapse stability.

NR3A has associations with neurodevelopmental disorders and neurodegenerative diseases. It is implicated in conditions such as schizophrenia where altered NR3A expression or function may contribute to cognitive deficits. Changes in NR3A levels relate to disruptions in NMDA receptor-mediated neurotransmission and could be linked to abnormal synaptic connectivity. Additionally NR3A might connect to Alzheimer's disease through pathways involving altered NMDA receptor activity where NR1 and NR2 subunits also play critical roles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of a non-conventional N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with low calcium permeability and low voltage-dependent block by Mg(2+). During the development of neural circuits, participates in the synaptic refinement period, restricting spine maturation and growth (By similarity). Forms glutamatergic receptor complexes with GluN1 and GluN2 subunits which are activated by glycine binding to the GluN1 and GluN3 subunits and L-glutamate binding to GluN2 subunits (By similarity). Forms excitatory glycinergic receptor complexes with GluN1 alone which are activated by glycine binding to the GluN1 and GluN3 subunits (By similarity). GluN3A subunit also binds D-serine (By similarity). Each GluN3 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (By similarity). By competing with GIT1 interaction with ARHGEF7/beta-PIX, may reduce GIT1/ARHGEF7-regulated local activation of RAC1, hence affecting signaling and limiting the maturation and growth of inactive synapses (PubMed : 24297929).

See full target information Grin3a

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NR3A antibody [EPR25287-60] - BSA and Azide free

Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (AB302517)

Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (AB302517)

Western blot - Anti-NR3A antibody [EPR25287-60] - BSA and Azide free (AB302517)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise