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AB327126

Anti-NR5A1 Antibody [CL9101]

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Mouse Monoclonal Steroidogenic Factor 1/SF-1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human NR5A1.

View Alternative Names

AD4BP, FTZF1, SF1, NR5A1, Steroidogenic factor 1, SF-1, STF-1, hSF-1, Adrenal 4-binding protein, Fushi tarazu factor homolog 1, Nuclear receptor subfamily 5 group A member 1, Steroid hormone receptor Ad4BP

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR5A1 Antibody [CL9101] (AB327126)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR5A1 Antibody [CL9101] (AB327126)

Immunohistochemical staining of human breast using ab327126 at 1 : 50–1 : 200 dilution, showing weak to moderate nuclear positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR5A1 Antibody [CL9101] (AB327126)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR5A1 Antibody [CL9101] (AB327126)

Immunohistochemical staining of human ovary using ab327126 at 1 : 50–1 : 200 dilution, showing moderate positivity in stromal cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR5A1 Antibody [CL9101] (AB327126)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR5A1 Antibody [CL9101] (AB327126)

Immunohistochemical staining of human testis using ab327126 at 1 : 50–1 : 200 dilution, showing weak to moderate nuclear positivity in Leydig cells and cells in seminiferous ducts.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR5A1 Antibody [CL9101] (AB327126)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR5A1 Antibody [CL9101] (AB327126)

Immunohistochemical staining of human adrenal gland using ab327126 at 1 : 50–1 : 200 dilution, showing moderate nuclear positivity in glandular cells.

Immunocytochemistry/ Immunofluorescence - Anti-NR5A1 Antibody [CL9101] (AB327126)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NR5A1 Antibody [CL9101] (AB327126)

Immunofluorescence staining of U-2 OS cells using ab327126 at 2–10 µg/ml, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Western blot - Anti-NR5A1 Antibody [CL9101] (AB327126)
  • WB

Supplier Data

Western blot - Anti-NR5A1 Antibody [CL9101] (AB327126)

Western blot analysis in human cell line HEL using ab327126 at 1 µg/ml.

All lanes:

Western blot - Anti-NR5A1 Antibody [CL9101] (ab327126)

All lanes:

Human cell line HEL

Predicted band size: 51.64 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR5A1 Antibody [CL9101] (AB327126)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NR5A1 Antibody [CL9101] (AB327126)

Immunohistochemical staining of human liver using ab327126 at 1 : 50–1 : 200 dilution, showing no positivity in hepatocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL9101

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human NR5A1.

Q13285

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator. Essential for sexual differentiation and formation of the primary steroidogenic tissues (PubMed : 27378692). Binds to the Ad4 site found in the promoter region of steroidogenic P450 genes such as CYP11A, CYP11B and CYP21B. Also regulates the AMH/Muellerian inhibiting substance gene as well as the AHCH and STAR genes. 5'-YCAAGGYC-3' and 5'-RRAGGTCA-3' are the consensus sequences for the recognition by NR5A1 (PubMed : 27378692). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional activity. Binds phosphatidylcholine (By similarity). Binds phospholipids with a phosphatidylinositol (PI) headgroup, in particular PI(3,4)P2 and PI(3,4,5)P3. Activated by the phosphorylation of NR5A1 by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation.
See full target information NR5A1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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