Rabbit Polyclonal NR5A2 / LRH1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR5A2 aa 100 to C-terminus.
Images
Publications
- Frontiers in cell and developmental biology 9:6435222021LRH1 Acts as an Oncogenic Driver in Human Osteosarcoma and Pan-Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Song,Weiwei An,Hongmei Wang,Yuanren Gao,Jihua Han,Chenguang Hao,Lin Chen,Shilong Liu,Ying XingPubMed 33791301
- eLife 8:2019Mutationally-activated PI3'-kinase-α promotes de-differentiation of lung tumors initiated by the BRAF oncoprotein kinase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Edward van Veen et. al.PubMed 31452510
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NR5A2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00482
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
| Chicken | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Xenopus tropicalis | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Cow, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (PubMed:20159957). Binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes, a critical cis-element of their expression and regulation. May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Activates the transcription of CYP2C38 (By similarity).
Alternative names
Nuclear receptor subfamily 5 group A member 2, Alpha-1-fetoprotein transcription factor, B1-binding factor, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, hB1F, LRH-1, CPF, FTF, NR5A2, B1F
Recommended products
Rabbit Polyclonal NR5A2 / LRH1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR5A2 aa 100 to C-terminus.
Alternative names
Nuclear receptor subfamily 5 group A member 2, Alpha-1-fetoprotein transcription factor, B1-binding factor, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, hB1F, LRH-1, CPF, FTF, NR5A2, B1F
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NR5A2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00482
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- Activity summary
NR5A2 also known as Liver Receptor Homolog-1 (LRH-1) is a nuclear receptor protein with a molecular weight of approximately 55 kDa. It regulates gene expression by binding to DNA response elements as a monomer. NR5A2 is expressed in the liver pancreas ovary and intestine. This receptor plays a central role in the transcriptional regulation of genes involved in important metabolic processes such as cholesterol homeostasis bile acid synthesis and steroidogenesis.
- Biological function summary
NR5A2 influences several physiological functions by modulating the expression of key genes. It is a part of the nuclear receptor superfamily and acts as a transcriptional regulator. This receptor directly impacts the regulation of genes that control metabolism and energy balance. Its role in lipid metabolism has been observed as NR5A2 regulates genes encoding enzymes for cholesterol and bile acid biosynthesis linking it to energy homeostasis.
- Pathways
The protein modulates essential biological processes involved in energy and lipid metabolism linking it to the Wnt/β-catenin signaling and cholesterol metabolism pathways. It interacts with signaling proteins such as β-catenin where it is involved in the transcriptional regulation of genes necessary for normal cellular function. NR5A2 through these pathways maintains homeostasis and influences cellular responses to metabolic changes highlighting its interaction within complex signaling networks.
- Associated diseases and disorders
NR5A2 has significant associations with metabolic diseases and cancers like pancreatic cancer. Mutations or dysregulations in NR5A2 expression levels can affect cholesterol metabolism potentially contributing to conditions such as non-alcoholic fatty liver disease (NAFLD). This receptor's expression levels influence the expression of genes that are pivotal in cancer development and its regulatory role may be linked to c-Myc a protein involved in cell growth and proliferation within these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NR5A2 / LRH1 antibody (ab153944)
7.5% SDS PAGE
All lanes: Western blot - Anti-NR5A2 / LRH1 antibody (ab153944) at 1/1000 dilution
All lanes: Mouse liver whole cell lysate at 50 µg
Predicted band size: 61 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NR5A2 / LRH1 antibody (ab153944)
Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, labeling NR5A2 / LRH1 with ab153944 at 1/400 dilution. Blue: Hoechst 33342 staining.
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