AB223211

Anti-NR5A2 / LRH1 antibody

Name: Anti-NR5A2 / LRH1 antibody (ab223211) | Abcam
Brand: Abcam Limited
SKU: AB223211
Price: 525 USD
Availability: InStock

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(2 Publications)

Goat Polyclonal NR5A2 / LRH1 antibody. Suitable for WB and reacts with Pig, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NR5A2 aa 200-250.

View Alternative Names

B1F, CPF, FTF, NR5A2, Nuclear receptor subfamily 5 group A member 2, Alpha-1-fetoprotein transcription factor, B1-binding factor, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, hB1F, LRH-1

2 Images

Supplier Data

Western blot - Anti-NR5A2 / LRH1 antibody (AB223211)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-NR5A2 / LRH1 antibody (ab223211) at 1 µg/mL

All lanes:

HepG2 (human liver hepatocellular carcinoma cell line) nuclear lysate (in RIPA buffer) at 35 µg

Predicted band size: 61 kDa

Observed band size: 55 kDa

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Supplier Data

Western blot - Anti-NR5A2 / LRH1 antibody (AB223211)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-NR5A2 / LRH1 antibody (ab223211) at 0.3 µg/mL

All lanes:

Pig ovary lysate (in RIPA buffer) at 35 µg

Predicted band size: 61 kDa

Observed band size: 60 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig

Applications

applications

Immunogen

Synthetic Peptide within Human NR5A2 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

O00482

Specificity

ab223211 is expected to recognize both reported isoforms (NP_995582.1; NP_003813.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab223211 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NR5A2 also known as Liver Receptor Homolog-1 (LRH-1) is a nuclear receptor protein with a molecular weight of approximately 55 kDa. It regulates gene expression by binding to DNA response elements as a monomer. NR5A2 is expressed in the liver pancreas ovary and intestine. This receptor plays a central role in the transcriptional regulation of genes involved in important metabolic processes such as cholesterol homeostasis bile acid synthesis and steroidogenesis.

Biological function summary

NR5A2 influences several physiological functions by modulating the expression of key genes. It is a part of the nuclear receptor superfamily and acts as a transcriptional regulator. This receptor directly impacts the regulation of genes that control metabolism and energy balance. Its role in lipid metabolism has been observed as NR5A2 regulates genes encoding enzymes for cholesterol and bile acid biosynthesis linking it to energy homeostasis.

Pathways

The protein modulates essential biological processes involved in energy and lipid metabolism linking it to the Wnt/β-catenin signaling and cholesterol metabolism pathways. It interacts with signaling proteins such as β-catenin where it is involved in the transcriptional regulation of genes necessary for normal cellular function. NR5A2 through these pathways maintains homeostasis and influences cellular responses to metabolic changes highlighting its interaction within complex signaling networks.

NR5A2 has significant associations with metabolic diseases and cancers like pancreatic cancer. Mutations or dysregulations in NR5A2 expression levels can affect cholesterol metabolism potentially contributing to conditions such as non-alcoholic fatty liver disease (NAFLD). This receptor's expression levels influence the expression of genes that are pivotal in cancer development and its regulatory role may be linked to c-Myc a protein involved in cell growth and proliferation within these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor : inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (PubMed : 20159957). May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Activates the transcription of CYP2C38 (By similarity).. (Microbial infection) Plays a crucial role for hepatitis B virus gene transcription and DNA replication. Mechanistically, synergistically cooperates with HNF1A to up-regulate the activity of one of the critical cis-elements in the hepatitis B virus genome enhancer II (ENII).

See full target information NR5A2

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Emergency medicine international 2022:1022078 PubMed37008288

2022

Abnormal Variations of the Key Genes in Osteoporotic Fractures.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Wang,Caiyuan Mai,Lei Pan

Frontiers in oncology 10:583566 PubMed33194722

2020

LRH1 Promotes Tumor Cell Proliferation and Migration and Is Correlated With Poor Prognosis in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wenzhou Sun,Qingtao Shi,Jiaxin Li,Jinmeng Li,Libo Yu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-NR5A2 / LRH1 antibody

Western blot - Anti-NR5A2 / LRH1 antibody (AB223211)

Western blot - Anti-NR5A2 / LRH1 antibody (AB223211)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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