AB189876

Anti-NR5A2 / LRH1 antibody - C-terminal

Name: Anti-NR5A2 / LRH1 antibody - C-terminal (ab189876) | Abcam
Brand: Abcam Limited
SKU: AB189876
Price: 525 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(8 Publications)

Rabbit Polyclonal NR5A2 / LRH1 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR5A2 aa 250 to C-terminus.

View Alternative Names

B1F, CPF, FTF, NR5A2, Nuclear receptor subfamily 5 group A member 2, Alpha-1-fetoprotein transcription factor, B1-binding factor, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, hB1F, LRH-1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NR5A2 / LRH1 antibody - C-terminal (AB189876)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NR5A2 / LRH1 antibody - C-terminal (AB189876)

Immunocytochemistry/Immunofluorescence analysis of A549 cells using ab189876. Blue DAPI for nuclear staining.

Supplier Data

Western blot - Anti-NR5A2 / LRH1 antibody - C-terminal (AB189876)

All lanes:

Western blot - Anti-NR5A2 / LRH1 antibody - C-terminal (ab189876) at 1/500 dilution

Lane 1:

MCF7 cell line extract

Lane 2:

293T cell line extract

Lane 3:

HeLa cell line extract

Lane 4:

Mouse liver extract

Lane 5:

Rat liver extract

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human NR5A2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O00482

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NR5A2 also known as Liver Receptor Homolog-1 (LRH-1) is a nuclear receptor protein with a molecular weight of approximately 55 kDa. It regulates gene expression by binding to DNA response elements as a monomer. NR5A2 is expressed in the liver pancreas ovary and intestine. This receptor plays a central role in the transcriptional regulation of genes involved in important metabolic processes such as cholesterol homeostasis bile acid synthesis and steroidogenesis.

Biological function summary

NR5A2 influences several physiological functions by modulating the expression of key genes. It is a part of the nuclear receptor superfamily and acts as a transcriptional regulator. This receptor directly impacts the regulation of genes that control metabolism and energy balance. Its role in lipid metabolism has been observed as NR5A2 regulates genes encoding enzymes for cholesterol and bile acid biosynthesis linking it to energy homeostasis.

Pathways

The protein modulates essential biological processes involved in energy and lipid metabolism linking it to the Wnt/β-catenin signaling and cholesterol metabolism pathways. It interacts with signaling proteins such as β-catenin where it is involved in the transcriptional regulation of genes necessary for normal cellular function. NR5A2 through these pathways maintains homeostasis and influences cellular responses to metabolic changes highlighting its interaction within complex signaling networks.

NR5A2 has significant associations with metabolic diseases and cancers like pancreatic cancer. Mutations or dysregulations in NR5A2 expression levels can affect cholesterol metabolism potentially contributing to conditions such as non-alcoholic fatty liver disease (NAFLD). This receptor's expression levels influence the expression of genes that are pivotal in cancer development and its regulatory role may be linked to c-Myc a protein involved in cell growth and proliferation within these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor : inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (PubMed : 20159957). May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Activates the transcription of CYP2C38 (By similarity).. (Microbial infection) Plays a crucial role for hepatitis B virus gene transcription and DNA replication. Mechanistically, synergistically cooperates with HNF1A to up-regulate the activity of one of the critical cis-elements in the hepatitis B virus genome enhancer II (ENII).

See full target information NR5A2

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 31:985-998 PubMed34652429

2021

The adeno-associated virus 2 genome and Rep 68/78 proteins interact with cellular sites of DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Boftsi,Fawn B Whittle,Juexin Wang,Phoenix Shepherd,Lisa R Burger,Kevin A Kaifer,Christian L Lorson,Trupti Joshi,David J Pintel,Kinjal Majumder

Frontiers in cell and developmental biology 9:666303 PubMed34631693

2021

Regulates Oviductal Epithelial Secretion Through the WNT Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Tan,Lingling Zhang,Tianqi Li,Jianmin Zhan,Kun Qiao,Haili Wu,Shenfei Sun,Meina Huang,Fangxi Zhang,Meixing Zhang,Changwei Li,Runsheng Li,Hongjie Pan

Frontiers in cell and developmental biology 9:643522 PubMed33791301

2021

LRH1 Acts as an Oncogenic Driver in Human Osteosarcoma and Pan-Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Song,Weiwei An,Hongmei Wang,Yuanren Gao,Jihua Han,Chenguang Hao,Lin Chen,Shilong Liu,Ying Xing

PLoS pathogens 16:e1009002 PubMed33064772

2020

The NS1 protein of the parvovirus MVM Aids in the localization of the viral genome to cellular sites of DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Kinjal Majumder,Maria Boftsi,Fawn B Whittle,Juexin Wang,Matthew S Fuller,Trupti Joshi,David J Pintel

OncoTargets and therapy 13:10231-10244 PubMed33116604

2020

Promotes Cell Growth and Resistance to Temozolomide Through Regulating Notch Signal Pathway in Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Quanxi Yang,Lei Deng,Jialiang Li,Pengfei Miao,Wenxiang Liu,Qi Huang

Molecular systems biology 15:e9043 PubMed31885203

2019

A gene regulatory network controls the balance between mesendoderm and ectoderm at pluripotency exit.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna L Sladitschek,Pierre A Neveu

Thoracic cancer 9:1725-1732 PubMed30273983

2018

LRH1 as a promising prognostic biomarker and predictor of metastasis in patients with non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuechao Liu,Ying Xing,Hongmei Wang,Shi Yan,Xinzhu Wang,Li Cai

eLife 7: PubMed30028293

2018

Parvovirus minute virus of mice interacts with sites of cellular DNA damage to establish and amplify its lytic infection.

Applications

Unspecified application

Species

Unspecified reactive species

Kinjal Majumder,Juexin Wang,Maria Boftsi,Matthew S Fuller,Jordan E Rede,Trupti Joshi,David J Pintel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NR5A2 / LRH1 antibody - C-terminal

Immunocytochemistry/ Immunofluorescence - Anti-NR5A2 / LRH1 antibody - C-terminal (AB189876)

Western blot - Anti-NR5A2 / LRH1 antibody - C-terminal (AB189876)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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