Rabbit Polyclonal NR5A2 / LRH1 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR5A2 aa 250 to C-terminus.
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Publications
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- Human molecular genetics 31:985-9982021The adeno-associated virus 2 genome and Rep 68/78 proteins interact with cellular sites of DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaria Boftsi,Fawn B Whittle,Juexin Wang,Phoenix Shepherd,Lisa R Burger,Kevin A Kaifer,Christian L Lorson,Trupti Joshi,David J Pintel,Kinjal MajumderPubMed 34652429
- Frontiers in cell and developmental biology 9:6663032021Regulates Oviductal Epithelial Secretion Through the WNT Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXue Tan,Lingling Zhang,Tianqi Li,Jianmin Zhan,Kun Qiao,Haili Wu,Shenfei Sun,Meina Huang,Fangxi Zhang,Meixing Zhang,Changwei Li,Runsheng Li,Hongjie PanPubMed 34631693
- Frontiers in cell and developmental biology 9:6435222021LRH1 Acts as an Oncogenic Driver in Human Osteosarcoma and Pan-Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Song,Weiwei An,Hongmei Wang,Yuanren Gao,Jihua Han,Chenguang Hao,Lin Chen,Shilong Liu,Ying XingPubMed 33791301
- PLoS pathogens 16:e10090022020The NS1 protein of the parvovirus MVM Aids in the localization of the viral genome to cellular sites of DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKinjal Majumder et. al.PubMed 33064772
- OncoTargets and therapy 13:10231-102442020Promotes Cell Growth and Resistance to Temozolomide Through Regulating Notch Signal Pathway in Glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQuanxi Yang et. al.PubMed 33116604
- Molecular systems biology 15:e90432019A gene regulatory network controls the balance between mesendoderm and ectoderm at pluripotency exit.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanna L Sladitschek,Pierre A NeveuPubMed 31885203
- Thoracic cancer 9:1725-17322018LRH1 as a promising prognostic biomarker and predictor of metastasis in patients with non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuechao Liu et. al.PubMed 30273983
- eLife 7:2018Parvovirus minute virus of mice interacts with sites of cellular DNA damage to establish and amplify its lytic infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKinjal Majumder,Juexin Wang,Maria Boftsi,Matthew S Fuller,Jordan E Rede,Trupti Joshi,David J PintelPubMed 30028293
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NR5A2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00482
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Rat | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Nuclear receptor that acts as a key metabolic sensor by regulating the expression of genes involved in bile acid synthesis, cholesterol homeostasis and triglyceride synthesis. Together with the oxysterol receptors NR1H3/LXR-alpha and NR1H2/LXR-beta, acts as an essential transcriptional regulator of lipid metabolism. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (PubMed:20159957). May be responsible for the liver-specific activity of enhancer II, probably in combination with other hepatocyte transcription factors. Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver. May also contribute to the regulation of pancreas-specific genes and play important roles in embryonic development. Activates the transcription of CYP2C38 (By similarity). (Microbial infection) Plays a crucial role for hepatitis B virus gene transcription and DNA replication. Mechanistically, synergistically cooperates with HNF1A to up-regulate the activity of one of the critical cis-elements in the hepatitis B virus genome enhancer II (ENII).
Alternative names
B1F, CPF, FTF, NR5A2, Nuclear receptor subfamily 5 group A member 2, Alpha-1-fetoprotein transcription factor, B1-binding factor, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, hB1F, LRH-1
Recommended products
Rabbit Polyclonal NR5A2 / LRH1 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NR5A2 aa 250 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NR5A2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00482
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NR5A2 also known as Liver Receptor Homolog-1 (LRH-1) is a nuclear receptor protein with a molecular weight of approximately 55 kDa. It regulates gene expression by binding to DNA response elements as a monomer. NR5A2 is expressed in the liver pancreas ovary and intestine. This receptor plays a central role in the transcriptional regulation of genes involved in important metabolic processes such as cholesterol homeostasis bile acid synthesis and steroidogenesis.
- Biological function summary
NR5A2 influences several physiological functions by modulating the expression of key genes. It is a part of the nuclear receptor superfamily and acts as a transcriptional regulator. This receptor directly impacts the regulation of genes that control metabolism and energy balance. Its role in lipid metabolism has been observed as NR5A2 regulates genes encoding enzymes for cholesterol and bile acid biosynthesis linking it to energy homeostasis.
- Pathways
The protein modulates essential biological processes involved in energy and lipid metabolism linking it to the Wnt/β-catenin signaling and cholesterol metabolism pathways. It interacts with signaling proteins such as β-catenin where it is involved in the transcriptional regulation of genes necessary for normal cellular function. NR5A2 through these pathways maintains homeostasis and influences cellular responses to metabolic changes highlighting its interaction within complex signaling networks.
- Associated diseases and disorders
NR5A2 has significant associations with metabolic diseases and cancers like pancreatic cancer. Mutations or dysregulations in NR5A2 expression levels can affect cholesterol metabolism potentially contributing to conditions such as non-alcoholic fatty liver disease (NAFLD). This receptor's expression levels influence the expression of genes that are pivotal in cancer development and its regulatory role may be linked to c-Myc a protein involved in cell growth and proliferation within these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-NR5A2 / LRH1 antibody - C-terminal (ab189876)
Immunocytochemistry/Immunofluorescence analysis of A549 cells using ab189876. Blue DAPI for nuclear staining.
Western blot - Anti-NR5A2 / LRH1 antibody - C-terminal (ab189876)
All lanes: Western blot - Anti-NR5A2 / LRH1 antibody - C-terminal (ab189876) at 1/500 dilution
Lane 1: MCF7 cell line extract
Lane 2: 293T cell line extract
Lane 3: HeLa cell line extract
Lane 4: Mouse liver extract
Lane 5: Rat liver extract
Predicted band size: 61 kDa
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