AB211448

Anti-NRAMP1 antibody

Name: Anti-NRAMP1 antibody (ab211448) | Abcam
Brand: Abcam Limited
SKU: AB211448
Rating: 5 (1 reviews)

(1 Review)

(7 Publications)

Rabbit Polyclonal NRAMP1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human SLC11A1 aa 250-300 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

LSH, NRAMP, NRAMP1, SLC11A1, Natural resistance-associated macrophage protein 1, NRAMP 1, Solute carrier family 11 member 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAMP1 antibody (AB211448)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAMP1 antibody (AB211448)

Immunohistochemical analysis of Human tonsil tissue labeling NRAMP1 with ab211448 at 5 μg/ml.

Supplier Data

Western blot - Anti-NRAMP1 antibody (AB211448)

All lanes:

Western blot - Anti-NRAMP1 antibody (ab211448) at 1/1000 dilution

All lanes:

CEM whole cell lysate at 35 µg

Predicted band size: 60 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SLC11A1 aa 250-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P49279

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NRAMP1 also known as Natural Resistance-Associated Macrophage Protein 1 or SLC11A1 functions as a metal ion transporter in the body and has a mass of approximately 62 kDa. This protein localizes mainly to macrophages and is a part of the membranes of phagosomes. Its role includes the transport of divalent metal ions like iron and manganese across cellular membranes contributing to ion homeostasis in cells.

Biological function summary

NRAMP1 plays a significant role in host defense mechanisms against intracellular pathogens. This protein modulates the immune response by controlling ion concentrations that affect pathogen replication. NRAMP1 does not function in isolation but interacts with the broader immune response network. It influences the innate immune system and is a part of macrophage activation processes that contribute to an organism's defense against infections.

Pathways

NRAMP1 impacts at least two major biological processes: the innate immune response and metal ion homeostasis pathways. It participates in the regulation of ion transport interacting with other proteins like DMT1 (another transporter for divalent metal ions) in balancing metal levels. These interactions are important in preserving cellular iron quotas and preventing oxidative damage through metal-catalyzed reactions.

NRAMP1 has associations with conditions such as tuberculosis and susceptibility to autoimmune diseases. Mutations or variations in NRAMP1 affect the body’s ability to combat Mycobacterium tuberculosis linking it to tuberculosis susceptibility. It also interacts with the protein TLR4 in pathways that influence inflammatory responses and potentially contribute to the development of autoimmune diseases by altering immune responses to prevent overreaction or ineffective clearing of pathogens.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Macrophage-specific antiporter that fluxes metal ions in either direction against a proton gradient. Localized to late endosomal lysosomal membranes, delivers bivalent cations from the cytosol into these acidic compartments where they may directly affect antimicrobial activity (PubMed : 11237855). Involved in iron metabolism and host natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes (Probable).

See full target information SLC11A1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Microorganisms 12: PubMed39065185

2024

Exosomal Small RNA Sequencing Profiles in Plasma from Subjects with Latent Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaogang Cui,Hangting Meng,Miao Li,Xia Chen,Dan Yuan,Changxin Wu

American journal of cancer research 14:2839-2851 PubMed39005670

2024

SLC11A1 predicts the overall survival of patients with colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hung-Chih Hsu,Yun-Shien Lee,Titilianty I Imbang,Ting-Chia Liu,Shuen-Iu Hung

World journal of oncology 15:662-674 PubMed38993257

2024

Significance and Possible Biological Mechanism for Downregulation in Kidney Renal Clear Cell Carcinoma Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Han Chu Ji,Jian Di Li,Guan Lan Zhang,Zhi Guang Huang,Ji Wen Cheng,Sheng Hua Li,Chun Yan Zhao,Yu Xing Tang,Kai Qin,You Liang Ma,Yu Long,Gang Chen,Bin Qin

The journal of gene medicine 26:e3684 PubMed38618694

2024

Constructing and validating a risk model based on neutrophil-related genes for evaluating prognosis and guiding immunotherapy in colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shasha Wang,Lili Wang,Mingxiu Qiu,Zhongkun Lin,Weiwei Qi,Jing Lv,Yan Wang,Yangyang Lu,Xiaoxuan Li,Wenzhi Chen,Wensheng Qiu

Bioscience reports 44: PubMed38419527

2024

Integrative analysis of metabolism subtypes and identification of prognostic metabolism-related genes for glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiahui Li,Yutian Wei,Jiali Liu,Shupeng Cheng,Xia Zhang,Huaide Qiu,Jianan Li,Chuan He

Frontiers in cell and developmental biology 11:1161667 PubMed37745305

2023

Identification and verification of a prognostic signature based on a miRNA-mRNA interaction pattern in colon adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiwu Zhao,Haosheng Li,Wenchang Li,Zichao Guo,Wenqing Jia,Shuiyu Xu,Sixia Chen,Xiaonan Shen,Changgang Wang

Frontiers in immunology 13:980378 PubMed36531992

2022

SLC11A1 as a stratification indicator for immunotherapy or chemotherapy in patients with glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Houshi Xu,Anke Zhang,Chaoyou Fang,Qingwei Zhu,Wei Wang,Yibo Liu,Zeyu Zhang,Xiaoyu Wang,Ling Yuan,Yuanzhi Xu,Anwen Shao,Meiqing Lou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NRAMP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRAMP1 antibody (AB211448)

Western blot - Anti-NRAMP1 antibody (AB211448)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise