AB77392

Anti-NRAS antibody

Name: Anti-NRAS antibody. Goat polyclonal (ab77392) | Abcam
Brand: Abcam Limited
SKU: AB77392
Price: 535 USD
Availability: InStock
Rating: 4 (11 reviews)

(11 Reviews)

(21 Publications)

Goat Polyclonal RASN antibody. Suitable for WB and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Synthetic Peptide within Human NRAS aa 150 to C-terminus.

View Alternative Names

HRAS1, NRAS, GTPase NRas, Transforming protein N-Ras

1 Images

Supplier Data

Western blot - Anti-NRAS antibody (AB77392)

Primary incubation for 1 hour at room temperature. Detected by chemiluminescence.

All lanes:

Western blot - Anti-NRAS antibody (ab77392) at 0.5 µg/mL

All lanes:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate in RIPA buffer. at 35 µg

Predicted band size: 21 kDa

Observed band size: 21 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human NRAS aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P01111

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation at room temperature is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab77392 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The NRAS protein also known as neuroblastoma RAS viral oncogene homolog has a molecular weight of approximately 24 kDa. It functions mechanically as a GTPase cycling between an inactive GDP-bound state and an active GTP-bound state. The NRAS gene which encodes this protein shows expression in various tissues including the thymus leukocytes and other cells of the hematopoietic lineage. Activation of RAS involves the conversion to the GTP-bound form which enables NRAS to transmit signals within cells.

Biological function summary

NRAS plays an important role in cell proliferation differentiation and survival. It does not function alone but is part of the larger RAS protein family including HRAS and KRAS with whom it shares similar roles and sequence homology. NRAS interacts with various growth factor receptors and mediates signals to downstream effectors that influence cellular processes. Mutations in the NRAS gene can affect these biological processes leading to altered cell behavior.

Pathways

NRAS has significant roles in the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways regulate fundamental cellular functions including growth and survival. NRAS interacts closely with other proteins such as RAF kinases and PI3K to propagate signals from activated receptors at the cell membrane to the nucleus. Altered NRAS activity can impact these pathways affecting cellular responses to external stimuli.

NRAS mutations are linked to certain cancers including melanoma and acute myeloid leukemia (AML). Mutant forms of NRAS result in constitutively activated RAS contributing to uncontrolled cell growth and survival. In melanoma NRAS mutations frequently co-occur with alterations in other proteins such as BRAF another member of the RAS/RAF/MEK/ERK pathway. Understanding NRAS's involvement in these conditions is critical for developing targeted therapeutic strategies.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

See full target information NRAS

style

left-column

Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Biochemistry and biophysics reports 41:101894 PubMed39896109

2025

Resistance to FLT3 inhibitors involves different molecular mechanisms and reduces new DNA synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingmei Yang,Ran Friedman

Cell reports. Medicine 5:101663 PubMed39094577

2024

In vivo vulnerabilities to GPX4 and HDAC inhibitors in drug-persistent versus drug-resistant BRAF lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Julie Nokin,Elodie Darbo,Elodie Richard,Sonia San José,Sergio de Hita,Valérie Prouzet-Mauleon,Béatrice Turcq,Laura Gerardelli,Rebekah Crake,Valérie Velasco,Benjamin Koopmansch,Frederic Lambert,Jenny Y Xue,Ben Sang,Julie Horne,Eric Ziemons,Alberto Villanueva,Arnaud Blomme,Michael Herfs,Didier Cataldo,Olivier Calvayrac,Paolo Porporato,Ernest Nadal,Piro Lito,Pasi A Jänne,Biagio Ricciuti,Mark M Awad,Chiara Ambrogio,David Santamaría

The Journal of clinical investigation 134: PubMed38875287

2024

Liver cancer initiation requires translational activation by an oncofetal regulon involving LIN28 proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Hsiung Hsieh,Yonglong Wei,Lin Li,Liem H Nguyen,Yu-Hsuan Lin,Jung M Yong,Xuxu Sun,Xun Wang,Xin Luo,Sarah K Knutson,Christina Bracken,George Q Daley,John T Powers,Hao Zhu

iScience 27:109802 PubMed38746666

2024

Discovery of SOCS7 as a versatile E3 ligase for protein-based degraders.

Applications

Unspecified application

Species

Unspecified reactive species

Anaïs Cornebois,Marie Sorbara,Margot Cristol,Emmanuelle Vigne,Pierre Cordelier,Klervi Desrumeaux,Nicolas Bery

Pigment cell & melanoma research 36:365-377 PubMed37341054

2023

The OSUMMER lines: A series of ultraviolet-accelerated NRAS-mutant mouse melanoma cell lines syngeneic to C57BL/6.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon M Murphy,Daelin M Jensen,Tiffany E Arnold,Renan Aguilar-Valenzuela,Jase Hughes,Valentina Posada,Kimberly T Nguyen,Vi T Chu,Kenneth Y Tsai,Craig J Burd,Christin E Burd

Cell reports 41:111623 PubMed36351408

2022

NF1 loss of function as an alternative initiating event in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Gopalakrishnan Ramakrishnan,Parash Parajuli,Pura Singh,Creighton Friend,Eric Hurwitz,Celine Prunier,Mohammed S Razzaque,Keli Xu,Azeddine Atfi

Clinical cancer research : an official journal of the American Association for Cancer Research 28:5343-5358 PubMed36222846

2022

The Mutational Landscape of Cancer's Vulnerability to Ionizing Radiation.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka Gopal,Brian D Yard,Aaron Petty,Jessica C Lal,Titas K Bera,Trung Q Hoang,Alexandru D Buhimschi,Mohamed E Abazeed

Nature communications 13:3153 PubMed35672316

2022

Enhanced BRAF engagement by NRAS mutants capable of promoting melanoma initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon M Murphy,Elizabeth M Terrell,Venkat R Chirasani,Tirzah J Weiss,Rachel E Lew,Andrea M Holderbaum,Aastha Dhakal,Valentina Posada,Marie Fort,Michael S Bodnar,Leiah M Carey,Min Chen,Craig J Burd,Vincenzo Coppola,Deborah K Morrison,Sharon L Campbell,Christin E Burd

Cell death discovery 8:271 PubMed35614051

2022

GPR110 promotes progression and metastasis of triple-negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hye-Jung Nam,Yeon-Ju Kim,Jae-Hyeok Kang,Su-Jae Lee

Molecular metabolism 61:101507 PubMed35490865

2022

Neuronal miR-29a protects from obesity in adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Ma,Nicola Murgia,Yu Liu,Zixuan Li,Chaweewan Sirakawin,Ruslan Konovalov,Nikolai Kovzel,Yang Xu,Xuejia Kang,Anshul Tiwari,Patrick Malonza Mwangi,Donglei Sun,Holger Erfle,Witold Konopka,Qingxuan Lai,Syeda Sadia Najam,Ilya A Vinnikov

View all publications

style

left-column

style

left-column

style

right-column