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Anti-NRAS antibody [EPR25186-24] is a Rabbit Monoclonal antibody that is used in NRAS IP, Western Blot. Suitable for Human, Mouse, Rat samples.

Neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS) is a GTPase enzyme that converts GTP to GDP. NRAS plays a key role in cell signalling.

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Images

Western blot - Anti-NRAS antibody [EPR25186-24] (AB300431), expandable thumbnail
  • Immunoprecipitation - Anti-NRAS antibody [EPR25186-24] (AB300431), expandable thumbnail
  • Western blot - Anti-NRAS antibody [EPR25186-24] (AB300431), expandable thumbnail
  • Western blot - Anti-NRAS antibody [EPR25186-24] (AB300431), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Not recommended
Not recommended
Not recommended
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Tested
Rat
Expected
Not recommended
Not recommended
Not recommended
Tested
Recombinant full length protein - Human
Not recommended
Not recommended
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Species
Recombinant full length protein - Human
Dilution info
1/1000
Notes

-

Target data

Function

Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.

Alternative names

GTPase NRas, Transforming protein N-Ras, Nras

Recommended products

Anti-NRAS antibody [EPR25186-24] is a Rabbit Monoclonal antibody that is used in NRAS IP, Western Blot. Suitable for Human, Mouse, Rat samples.

Neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS) is a GTPase enzyme that converts GTP to GDP. NRAS plays a key role in cell signalling.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25186-24
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The NRAS protein also known as neuroblastoma RAS viral oncogene homolog has a molecular weight of approximately 24 kDa. It functions mechanically as a GTPase cycling between an inactive GDP-bound state and an active GTP-bound state. The NRAS gene which encodes this protein shows expression in various tissues including the thymus leukocytes and other cells of the hematopoietic lineage. Activation of RAS involves the conversion to the GTP-bound form which enables NRAS to transmit signals within cells.

Biological function summary

NRAS plays an important role in cell proliferation differentiation and survival. It does not function alone but is part of the larger RAS protein family including HRAS and KRAS with whom it shares similar roles and sequence homology. NRAS interacts with various growth factor receptors and mediates signals to downstream effectors that influence cellular processes. Mutations in the NRAS gene can affect these biological processes leading to altered cell behavior.

Pathways

NRAS has significant roles in the MAPK/ERK pathway and the PI3K/AKT pathway. These pathways regulate fundamental cellular functions including growth and survival. NRAS interacts closely with other proteins such as RAF kinases and PI3K to propagate signals from activated receptors at the cell membrane to the nucleus. Altered NRAS activity can impact these pathways affecting cellular responses to external stimuli.

Associated diseases and disorders

NRAS mutations are linked to certain cancers including melanoma and acute myeloid leukemia (AML). Mutant forms of NRAS result in constitutively activated RAS contributing to uncontrolled cell growth and survival. In melanoma NRAS mutations frequently co-occur with alterations in other proteins such as BRAF another member of the RAS/RAF/MEK/ERK pathway. Understanding NRAS's involvement in these conditions is critical for developing targeted therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-NRAS antibody [EPR25186-24] (ab300431), expandable thumbnail

    Western blot - Anti-NRAS antibody [EPR25186-24] (ab300431)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, ab300431 was shown to bind specifically to NRAS.

    One band was observed at 21 kDa in wild-type HEK-293T (ab255449) cell lysates with no signal observed at this size in NRAS knockout cell lysates (Human NRAS knockout HEK-293T cell lysate ab258542).

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-NRAS antibody [EPR25186-24] (ab300431) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T (ab255449) whole cell lysate

    Lane 2: NRAS knockout HEK-293T (Human NRAS knockout HEK-293T cell line ab266684) whole cell lysate

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 21 kDa

    Exposure time: 180s

  • Immunoprecipitation - Anti-NRAS antibody [EPR25186-24] (ab300431), expandable thumbnail

    Immunoprecipitation - Anti-NRAS antibody [EPR25186-24] (ab300431)

    NRAS was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug with ab300431 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300431 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate 10 ug

    Lane 2: ab300431 IP in MCF7 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab300431 in MCF7 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Immunoprecipitation - Anti-NRAS antibody [EPR25186-24] (ab300431) at 1/1000 dilution

    Lanes 1 - 2: MCF7 whole cell lysate

    Lane 3: Immunoprecipitation - Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Developed using the ECL technique.

    Exposure time: 180s

  • Western blot - Anti-NRAS antibody [EPR25186-24] (ab300431), expandable thumbnail

    Western blot - Anti-NRAS antibody [EPR25186-24] (ab300431)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-NRAS antibody [EPR25186-24] (ab300431) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 40 µg

    Lane 2: A375 (human malignant melanoma epithelial cell) whole cell lysate at 40 µg

    Lane 3: C6 (rat glial tumor glial cell) whole cell lysate at 40 µg

    Lane 4: Rat spleen tissue lysate at 40 µg

    Lane 5: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 40 µg

    Lane 6: Mouse spleen tissue lysate at 40 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 21 kDa

    Exposure time: 15s

  • Western blot - Anti-NRAS antibody [EPR25186-24] (ab300431), expandable thumbnail

    Western blot - Anti-NRAS antibody [EPR25186-24] (ab300431)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    This antibody does not cross-react with human KRAS or HRAS.

    All lanes: Western blot - Anti-NRAS antibody [EPR25186-24] (ab300431) at 1/1000 dilution

    Lane 1: Human KRAS recombinant protein

    Lane 2: His-tagged human HRAS recombinant protein

    Lane 3: His-tagged human NRAS recombinant protein

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 21 kDa

    Exposure time: 37s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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