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AB175366

Anti-NrCAM antibody [7D8C5] - Neuronal Marker

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(1 Publication)

Mouse Monoclonal NRCAM antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NRCAM aa 1150-1300.

View Alternative Names

KIAA0343, NRCAM, Neuronal cell adhesion molecule, Nr-CAM, Neuronal surface protein Bravo, NgCAM-related cell adhesion molecule, hBravo, Ng-CAM-related

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-NrCAM antibody [7D8C5] - Neuronal Marker (AB175366)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NrCAM antibody [7D8C5] - Neuronal Marker (AB175366)

Immunofluorescent analysis of HepG2 cells labeling NrCAM using ab175366 at 1/50 dilution (green).

Blue : DRAQ5 fluorescent DNA dye.

Red : Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot - Anti-NrCAM antibody [7D8C5] - Neuronal Marker (AB175366)
  • WB

Supplier Data

Western blot - Anti-NrCAM antibody [7D8C5] - Neuronal Marker (AB175366)

Expected MW is 32.7 kDa

All lanes:

Western blot - Anti-NrCAM antibody [7D8C5] - Neuronal Marker (ab175366) at 1/500 dilution

Lane 1:

non transfected HEK293 cell lysate.

Lane 2:

NrCAM (aa 1192-1255)-hIgGFc transfected HEK293 cell lysate.

Predicted band size: 144 kDa

false

Western blot - Anti-NrCAM antibody [7D8C5] - Neuronal Marker (AB175366)
  • WB

Supplier Data

Western blot - Anti-NrCAM antibody [7D8C5] - Neuronal Marker (AB175366)

Expected MW is 32.7 kDa

All lanes:

Western blot - Anti-NrCAM antibody [7D8C5] - Neuronal Marker (ab175366) at 1/500 dilution

All lanes:

Human NrCAM recombinant protein

Predicted band size: 144 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7D8C5

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human NRCAM aa 1150-1300. The exact immunogen used to generate this antibody is proprietary information.

Q92823

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NrCAM also known as Neuronal Cell Adhesion Molecule is a protein involved in cell-cell adhesion. It is part of the immunoglobulin superfamily and has a molecular weight of approximately 135 kDa. NrCAM is expressed mainly in the nervous system including areas like the brain and spinal cord. It plays a role in the development and maintenance of neural connections acting as a conduit between neurons.
Biological function summary

NrCAM functions in neural development and synaptic plasticity. It is not only a standalone protein but forms complexes with other adhesion molecules to maintain neural circuit integrity. Through its interactions NrCAM supports processes like axon guidance and dendritic spine formation. It also participates in signaling pathways that regulate these neural processes contributing to the dynamic environment necessary for brain development and function.

Pathways

NrCAM is involved in the formation of neural networks and cellular signaling cascades. It links to pathways such as the axon guidance pathway and neuronal signaling cascade. Through these routes it interacts with proteins like L1CAM and contactin which are pivotal in promoting correct neural patterning. Together these proteins facilitate the direction and bonding between neurons ensuring the establishment of proper neural connections.

NrCAM has associations with neurological conditions like autism spectrum disorder and schizophrenia. Research indicates that alterations in NrCAM expression or function may contribute to the symptoms observed in these disorders. It links with proteins such as DISC1 in schizophrenia context affecting pathways that influence brain connectivity and cognitive function. Understanding NrCAM's role could lead to advancements in therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier.
See full target information NRCAM
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:425 PubMed35501353

2022

Mxi1 participates in the progression of lung cancer via the microRNA-300/KLF9/GADD34 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yujie Lei,Yunchao Huang,Jianbin Lin,Shihui Sun,Keda Che,Junting Shen,Jun Liao,Yangming Chen,Kai Chen,Zhaoxian Lin,Xing Lin
View all publications
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Product promise

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