Anti-NrCAM antibody [EPR15654] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NrCAM antibody [EPR15654] - BSA and Azide free (AB251072)

Immunohistochemical analysis of paraffin-embedded human liver tissue labelling NrCAM with ab191418 at 1/500 dilution (0.89 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody at a ready to use concentration. Negative control : no staining on human liver. The section was incubated with ab191418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. This data was developed using ab191418, the same antibody clone in a different buffer formulation.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NrCAM antibody [EPR15654] - BSA and Azide free (AB251072)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling NrCAM with ab191418 at 1/500 dilution (0.89 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody at a ready to use concentration. Positive staining on human cerebrum. The section was incubated with ab191418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins This data was developed using ab191418, the same antibody clone in a different buffer formulation.

• IP

Supplier Data

Immunoprecipitation - Anti-NrCAM antibody [EPR15654] - BSA and Azide free (AB251072)

This data was developed using ab191418, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from Human glioma lysate immunoprecipitated using ab191418 at 1/30 dilution.
Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (<a href='/en-us/products/primary-antibodies/nrcam-antibody-epr15654-neuronal-marker-ab191418'>ab191418</a>)

Predicted band size: 144 kDa

false

• WB

Supplier Data

Western blot - Anti-NrCAM antibody [EPR15654] - BSA and Azide free (AB251072)

This data was developed using ab191418, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (<a href='/en-us/products/primary-antibodies/nrcam-antibody-epr15654-neuronal-marker-ab191418'>ab191418</a>) at 1/10000 dilution

All lanes:

Human forebrain lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 144 kDa

false

• WB

Supplier Data

Western blot - Anti-NrCAM antibody [EPR15654] - BSA and Azide free (AB251072)

This data was developed using ab191418, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-NrCAM antibody [EPR15654] - Neuronal Marker (<a href='/en-us/products/primary-antibodies/nrcam-antibody-epr15654-neuronal-marker-ab191418'>ab191418</a>) at 1/50000 dilution

All lanes:

Human glioma lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 144 kDa,30 kDa

false