AB24344

Anti-NrCAM antibody - Neuronal Marker

Name: Anti-NrCAM antibody - Neuronal Marker (ab24344) | Abcam
Brand: Abcam Limited
SKU: AB24344
Price: 400 USD
Availability: InStock
Rating: 3 (2 reviews)

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What is this?

(2 Reviews)

(46 Publications)

Rabbit Polyclonal NRCAM antibody. Neuron marker. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 46 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Nrcam.

View Alternative Names

Kiaa0343, Nrcam, Neuronal cell adhesion molecule, Nr-CAM, Neuronal surface protein Bravo, NgCAM-related cell adhesion molecule, mBravo, Ng-CAM-related

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NrCAM antibody - Neuronal Marker (AB24344)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NrCAM antibody - Neuronal Marker (AB24344)

ab24344 detects NrCAM by immunofluorescence on embryonic day 11.5 mouse spinal cord. Floor plate commissurral axons crossing to the contralateral side of the spinal cord are shown here labeled for NrCAM by ab24344 (1/300). Mouse spinal cord was 4% PFA-fixed and processed on slide for IF. Scale bar=50um

This image is courtesy of Martin Grumet, Rutgers University, United States

Unknown

Western blot - Anti-NrCAM antibody - Neuronal Marker (AB24344)

ab24344 also can be used for immunoprecipatation and blocks function

All lanes:

Western blot - Anti-NrCAM antibody - Neuronal Marker (ab24344) at 1/500 dilution

All lanes:

20 µg of postnatal day 8 mouse brain extract in SDS

Predicted band size: 144 kDa

Observed band size: 145 kDa

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This image is courtesy of Martin Grumet, Rutgers University, United States

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Mouse Nrcam. The exact immunogen used to generate this antibody is proprietary information.

Q810U4

Specificity

ab24344 immunoblots a single major protein of NrCAM (see western blot image on datasheet) and recognizes proteins of comparable size from mouse, rat and human.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }

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Product details

This antibody recognizes an extracellular region of the protein. It cross-reacts with human Fc region.

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NrCAM also known as Neuronal Cell Adhesion Molecule is a protein involved in cell-cell adhesion. It is part of the immunoglobulin superfamily and has a molecular weight of approximately 135 kDa. NrCAM is expressed mainly in the nervous system including areas like the brain and spinal cord. It plays a role in the development and maintenance of neural connections acting as a conduit between neurons.

Biological function summary

NrCAM functions in neural development and synaptic plasticity. It is not only a standalone protein but forms complexes with other adhesion molecules to maintain neural circuit integrity. Through its interactions NrCAM supports processes like axon guidance and dendritic spine formation. It also participates in signaling pathways that regulate these neural processes contributing to the dynamic environment necessary for brain development and function.

Pathways

NrCAM is involved in the formation of neural networks and cellular signaling cascades. It links to pathways such as the axon guidance pathway and neuronal signaling cascade. Through these routes it interacts with proteins like L1CAM and contactin which are pivotal in promoting correct neural patterning. Together these proteins facilitate the direction and bonding between neurons ensuring the establishment of proper neural connections.

NrCAM has associations with neurological conditions like autism spectrum disorder and schizophrenia. Research indicates that alterations in NrCAM expression or function may contribute to the symptoms observed in these disorders. It links with proteins such as DISC1 in schizophrenia context affecting pathways that influence brain connectivity and cognitive function. Understanding NrCAM's role could lead to advancements in therapeutic strategies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding (PubMed : 11564762). Plays a role in mediating cell-cell contacts between Schwann cells and axons (PubMed : 20188654). Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters (PubMed : 14602817, PubMed : 20188654). Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier (PubMed : 24719088).

See full target information Nrcam

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Publications (46)

Recent publications for all applications. Explore the full list and refine your search

Current research in neurobiology 8:100150 PubMed40276719

2025

Regulation of perisomatic synapses from cholecystokinin basket interneurons through NrCAM and Ankyrin B.

Applications

Unspecified application

Species

Unspecified reactive species

Erik N Oldre,Barrett D Webb,Justin E Sperringer,Patricia F Maness

Genesis (New York, N.Y. : 2000) 62:e23596 PubMed38665067

2024

Following the p63/Keratin5 basal cells in the sensory and non-sensory epithelia of the vomeronasal organ.

Applications

Unspecified application

Species

Unspecified reactive species

Noah M LeFever,Raghu Ram Katreddi,Nikki M Dolphin,Nick A Mathias,Paolo E Forni

Neuroscience bulletin 40:1093-1103 PubMed38311706

2024

Abnormal Innervation, Demyelination, and Degeneration of Spiral Ganglion Neurons as Well as Disruption of Heminodes are Involved in the Onset of Deafness in Cx26 Null Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Qiu,Le Xie,Xiaohui Wang,Kai Xu,Xue Bai,Sen Chen,Yu Sun

Nature communications 14:6797 PubMed37884508

2023

Antibody-directed extracellular proximity biotinylation reveals that Contactin-1 regulates axo-axonic innervation of axon initial segments.

Applications

Unspecified application

Species

Unspecified reactive species

Yuki Ogawa,Brian C Lim,Shanu George,Juan A Oses-Prieto,Joshua M Rasband,Yael Eshed-Eisenbach,Hamdan Hamdan,Supna Nair,Francesco Boato,Elior Peles,Alma L Burlingame,Linda Van Aelst,Matthew N Rasband

Molecular neurodegeneration 18:13 PubMed36810097

2023

The Alzheimer's disease-linked protease BACE1 modulates neuronal IL-6 signaling through shedding of the receptor gp130.

Applications

Unspecified application

Species

Unspecified reactive species

Stephan A Müller,Merav D Shmueli,Xiao Feng,Johanna Tüshaus,Neele Schumacher,Ryan Clark,Brad E Smith,An Chi,Stefan Rose-John,Matthew E Kennedy,Stefan F Lichtenthaler

Frontiers in neuroscience 17:1100121 PubMed36793543

2023

Differential impacts of heterozygosity and null homozygosity on axon and myelinated fiber development in mouse.

Applications

Unspecified application

Species

Unspecified reactive species

Carmen Cifuentes-Diaz,Giorgia Canali,Marta Garcia,Mélanie Druart,Taylor Manett,Mythili Savariradjane,Camille Guillaume,Corentin Le Magueresse,Laurence Goutebroze

Nature communications 13:6040 PubMed36229455

2022

Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system.

Applications

Unspecified application

Species

Unspecified reactive species

Noemie Vilallongue,Julia Schaeffer,Anne-Marie Hesse,Céline Delpech,Béatrice Blot,Antoine Paccard,Elise Plissonnier,Blandine Excoffier,Yohann Couté,Stephane Belin,Homaira Nawabi

Nature communications 13:3196 PubMed35680881

2022

Proteomic and functional analyses of the periodic membrane skeleton in neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Ruobo Zhou,Boran Han,Roberta Nowak,Yunzhe Lu,Evan Heller,Chenglong Xia,Athar H Chishti,Velia M Fowler,Xiaowei Zhuang

PLoS biology 20:e3001590 PubMed35358180

2022

Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.

Applications

Unspecified application

Species

Unspecified reactive species

Erica L Gorenberg,Sofia Massaro Tieze,Betül Yücel,Helen R Zhao,Vicky Chou,Gregory S Wirak,Susumu Tomita,TuKiet T Lam,Sreeganga S Chandra

Nature 588:296-302 PubMed33177716

2020

Chemico-genetic discovery of astrocytic control of inhibition in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Tetsuya Takano,John T Wallace,Katherine T Baldwin,Alicia M Purkey,Akiyoshi Uezu,Jamie L Courtland,Erik J Soderblom,Tomomi Shimogori,Patricia F Maness,Cagla Eroglu,Scott H Soderling

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NrCAM antibody - Neuronal Marker

Immunocytochemistry/ Immunofluorescence - Anti-NrCAM antibody - Neuronal Marker (AB24344)

Western blot - Anti-NrCAM antibody - Neuronal Marker (AB24344)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

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Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (46)

Product promise