Rabbit Polyclonal NRCAM antibody. Neural Stem Cell marker. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 41 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Nrcam.
Images
Publications
Filter by
- Nature communications 14:67972023Antibody-directed extracellular proximity biotinylation reveals that Contactin-1 regulates axo-axonic innervation of axon initial segments.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuki Ogawa,Brian C Lim,Shanu George,Juan A Oses-Prieto,Joshua M Rasband,Yael Eshed-Eisenbach,Hamdan Hamdan,Supna Nair,Francesco Boato,Elior Peles,Alma L Burlingame,Linda Van Aelst,Matthew N RasbandPubMed 37884508
- Molecular neurodegeneration 18:132023The Alzheimer's disease-linked protease BACE1 modulates neuronal IL-6 signaling through shedding of the receptor gp130.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStephan A Müller,Merav D Shmueli,Xiao Feng,Johanna Tüshaus,Neele Schumacher,Ryan Clark,Brad E Smith,An Chi,Stefan Rose-John,Matthew E Kennedy,Stefan F LichtenthalerPubMed 36810097
- Frontiers in neuroscience 17:11001212023Differential impacts of heterozygosity and null homozygosity on axon and myelinated fiber development in mouse.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarmen Cifuentes-Diaz,Giorgia Canali,Marta Garcia,Mélanie Druart,Taylor Manett,Mythili Savariradjane,Camille Guillaume,Corentin Le Magueresse,Laurence GoutebrozePubMed 36793543
- Nature communications 13:60402022Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoemie Vilallongue,Julia Schaeffer,Anne-Marie Hesse,Céline Delpech,Béatrice Blot,Antoine Paccard,Elise Plissonnier,Blandine Excoffier,Yohann Couté,Stephane Belin,Homaira NawabiPubMed 36229455
- Nature communications 13:31962022Proteomic and functional analyses of the periodic membrane skeleton in neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuobo Zhou,Boran Han,Roberta Nowak,Yunzhe Lu,Evan Heller,Chenglong Xia,Athar H Chishti,Velia M Fowler,Xiaowei ZhuangPubMed 35680881
- PLoS biology 20:e30015902022Identification of substrates of palmitoyl protein thioesterase 1 highlights roles of depalmitoylation in disulfide bond formation and synaptic function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErica L Gorenberg,Sofia Massaro Tieze,Betül Yücel,Helen R Zhao,Vicky Chou,Gregory S Wirak,Susumu Tomita,TuKiet T Lam,Sreeganga S ChandraPubMed 35358180
- Nature 588:296-3022020Chemico-genetic discovery of astrocytic control of inhibition in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTetsuya Takano,John T Wallace,Katherine T Baldwin,Alicia M Purkey,Akiyoshi Uezu,Jamie L Courtland,Erik J Soderblom,Tomomi Shimogori,Patricia F Maness,Cagla Eroglu,Scott H SoderlingPubMed 33177716
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:2465-24822020Mouse brain proteomics establishes MDGA1 and CACHD1 as in vivo substrates of the Alzheimer protease BACE1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJasenka Rudan Njavro,Jakob Klotz,Bastian Dislich,Johanna Wanngren,Merav D Shmueli,Julia Herber,Peer-Hendrik Kuhn,Rohit Kumar,Thomas Koeglsperger,Marcus Conrad,Wolfgang Wurst,Regina Feederle,Andreas Vlachos,Stylianos Michalakis,Peter Jedlicka,Stephan A Müller,Stefan F LichtenthalerPubMed 31908000
- The Journal of neuroscience : the official journal 39:6233-62502019Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex Through Semaphorin 3B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVishwa Mohan et. al.PubMed 31182634
- EMBO molecular medicine 11:2019NrCAM is a marker for substrate-selective activation of ADAM10 in Alzheimer's disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTobias Brummer,Stephan A Müller,Francisco Pan-Montojo,Fumiaki Yoshida,Andreas Fellgiebel,Taisuke Tomita,Kristina Endres,Stefan F LichtenthalerPubMed 30833305
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Mouse Nrcam. The exact immunogen used to generate this antibody is proprietary information. Database link Q810U4
Consider this alternative
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Mouse | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding (PubMed:11564762). Plays a role in mediating cell-cell contacts between Schwann cells and axons (PubMed:20188654). Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters (PubMed:14602817, PubMed:20188654). Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier (PubMed:24719088).
Alternative names
Kiaa0343, Nrcam, Neuronal cell adhesion molecule, Nr-CAM, Neuronal surface protein Bravo, NgCAM-related cell adhesion molecule, mBravo, Ng-CAM-related
Recommended products
Rabbit Polyclonal NRCAM antibody. Neural Stem Cell marker. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 41 publications. Immunogen corresponding to Recombinant Fragment Protein within Mouse Nrcam.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: Whole serum
- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Mouse Nrcam. The exact immunogen used to generate this antibody is proprietary information. Database link Q810U4
- Purity
- Whole antiserum
- Specificity
ab24344 immunoblots a single major protein of NrCAM (see western blot image on datasheet) and recognizes proteins of comparable size from mouse, rat and human.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This antibody recognizes an extracellular region of the protein. It cross-reacts with human Fc region.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NrCAM also known as Neuronal Cell Adhesion Molecule is a protein involved in cell-cell adhesion. It is part of the immunoglobulin superfamily and has a molecular weight of approximately 135 kDa. NrCAM is expressed mainly in the nervous system including areas like the brain and spinal cord. It plays a role in the development and maintenance of neural connections acting as a conduit between neurons.
- Biological function summary
NrCAM functions in neural development and synaptic plasticity. It is not only a standalone protein but forms complexes with other adhesion molecules to maintain neural circuit integrity. Through its interactions NrCAM supports processes like axon guidance and dendritic spine formation. It also participates in signaling pathways that regulate these neural processes contributing to the dynamic environment necessary for brain development and function.
- Pathways
NrCAM is involved in the formation of neural networks and cellular signaling cascades. It links to pathways such as the axon guidance pathway and neuronal signaling cascade. Through these routes it interacts with proteins like L1CAM and contactin which are pivotal in promoting correct neural patterning. Together these proteins facilitate the direction and bonding between neurons ensuring the establishment of proper neural connections.
- Associated diseases and disorders
NrCAM has associations with neurological conditions like autism spectrum disorder and schizophrenia. Research indicates that alterations in NrCAM expression or function may contribute to the symptoms observed in these disorders. It links with proteins such as DISC1 in schizophrenia context affecting pathways that influence brain connectivity and cognitive function. Understanding NrCAM's role could lead to advancements in therapeutic strategies for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
This image is courtesy of Martin Grumet, Rutgers University, United StatesImmunocytochemistry/ Immunofluorescence - Anti-NrCAM antibody - Neuronal Marker (ab24344)
ab24344 detects NrCAM by immunofluorescence on embryonic day 11.5 mouse spinal cord. Floor plate commissurral axons crossing to the contralateral side of the spinal cord are shown here labeled for NrCAM by ab24344 (1/300). Mouse spinal cord was 4% PFA-fixed and processed on slide for IF. Scale bar=50um
This image is courtesy of Martin Grumet, Rutgers University, United StatesWestern blot - Anti-NrCAM antibody - Neuronal Marker (ab24344)
ab24344 also can be used for immunoprecipatation and blocks function
All lanes: Western blot - Anti-NrCAM antibody - Neuronal Marker (ab24344) at 1/500 dilution
All lanes: 20 µg of postnatal day 8 mouse brain extract in SDS
Predicted band size: 144 kDa
Observed band size: 145 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com