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AB186247

Anti-NrCAM antibody [S364-51] - Neuronal Marker

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Mouse Monoclonal NRCAM antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Nrcam aa 1-850.

View Alternative Names

KIAA0343, NRCAM, Neuronal cell adhesion molecule, Nr-CAM, Neuronal surface protein Bravo, NgCAM-related cell adhesion molecule, hBravo, Ng-CAM-related

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-NrCAM antibody [S364-51] - Neuronal Marker (AB186247)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NrCAM antibody [S364-51] - Neuronal Marker (AB186247)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling NrCAM with ab186247 at 1/100 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) NrCAM Antibody (D) Composite.

Immunocytochemistry/ Immunofluorescence - Anti-NrCAM antibody [S364-51] - Neuronal Marker (AB186247)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NrCAM antibody [S364-51] - Neuronal Marker (AB186247)

ab186247 staining NrCAM in SK-N-BE (Human neuroblastoma cell line) cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde for 15 minutes at room temperature. Samples were incubated with primary antibody at 1 : 100 dilution for 60 minutes at room temperature. An Atto 488 Goat anti-mouse was used as the secondary antibody at 1/100 dilution for 60 minutes at room temperature. Phalloidin Texas Red F-Actin was used as a counterstain. DAPI (blue) was used as a nuclear stain (A). Phalloidin Texas Red F-Actin stain (B), ab186247 (C) and merged (D).

Western blot - Anti-NrCAM antibody [S364-51] - Neuronal Marker (AB186247)
  • WB

Supplier Data

Western blot - Anti-NrCAM antibody [S364-51] - Neuronal Marker (AB186247)

Western Blot analysis of Rat Brain Membrane showing detection of ~160 kDa NrCam protein using Mouse Anti-NrCam Monoclonal Antibody, Clone S364-51 (ab186247). Load : 10 μg. Primary Antibody : Mouse Anti-NrCam Monoclonal Antibody (ab186247) at 1 : 1000 for 1 hour at RT. Secondary Antibody : Goat Anti-Mouse HRP at 1 : 200 for 1 hour at RT. Predicted/Observed Size : ~160 kDa.

All lanes:

Western blot - Anti-NrCAM antibody [S364-51] - Neuronal Marker (ab186247)

Predicted band size: 144 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

S364-51

Isotype

IgG2a

Carrier free

No

Reacts with

Human, Rat

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Mouse Nrcam aa 1-850. The exact immunogen used to generate this antibody is proprietary information.

Q810U4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.1% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NrCAM also known as Neuronal Cell Adhesion Molecule is a protein involved in cell-cell adhesion. It is part of the immunoglobulin superfamily and has a molecular weight of approximately 135 kDa. NrCAM is expressed mainly in the nervous system including areas like the brain and spinal cord. It plays a role in the development and maintenance of neural connections acting as a conduit between neurons.
Biological function summary

NrCAM functions in neural development and synaptic plasticity. It is not only a standalone protein but forms complexes with other adhesion molecules to maintain neural circuit integrity. Through its interactions NrCAM supports processes like axon guidance and dendritic spine formation. It also participates in signaling pathways that regulate these neural processes contributing to the dynamic environment necessary for brain development and function.

Pathways

NrCAM is involved in the formation of neural networks and cellular signaling cascades. It links to pathways such as the axon guidance pathway and neuronal signaling cascade. Through these routes it interacts with proteins like L1CAM and contactin which are pivotal in promoting correct neural patterning. Together these proteins facilitate the direction and bonding between neurons ensuring the establishment of proper neural connections.

NrCAM has associations with neurological conditions like autism spectrum disorder and schizophrenia. Research indicates that alterations in NrCAM expression or function may contribute to the symptoms observed in these disorders. It links with proteins such as DISC1 in schizophrenia context affecting pathways that influence brain connectivity and cognitive function. Understanding NrCAM's role could lead to advancements in therapeutic strategies for these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding. Plays a role in mediating cell-cell contacts between Schwann cells and axons. Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes; not required for the formation of mature nodes with normal sodium channel clusters. Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier.
See full target information NRCAM
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