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Knockout Tested Rabbit Recombinant Monoclonal NRDP1 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-NRDP1 antibody [EPR26281-19] (AB314642), expandable thumbnail
  • Western blot - Anti-NRDP1 antibody [EPR26281-19] (AB314642), expandable thumbnail
  • Immunoprecipitation - Anti-NRDP1 antibody [EPR26281-19] (AB314642), expandable thumbnail
  • Western blot - Anti-NRDP1 antibody [EPR26281-19] (AB314642), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Expected
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Human

Dilution info

1/30

Notes

-

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Acts as E3 ubiquitin-protein ligase and regulates the degradation of target proteins. Polyubiquitinates MYD88. Negatively regulates MYD88-dependent production of pro-inflammatory cytokines. Can promote TRIF-dependent production of type I interferon and inhibits infection with vesicular stomatitis virus (By similarity). Promotes also activation of TBK1 and IRF3. Involved in the ubiquitination of erythropoietin (EPO) and interleukin-3 (IL-3) receptors. Thus, through maintaining basal levels of cytokine receptors, RNF41 is involved in the control of hematopoietic progenitor cell differentiation into myeloerythroid lineages (By similarity). Contributes to the maintenance of steady-state ERBB3 levels by mediating its growth factor-independent degradation. Involved in the degradation of the inhibitor of apoptosis BIRC6 and thus is an important regulator of cell death by promoting apoptosis. Acts also as a PRKN modifier that accelerates its degradation, resulting in a reduction of PRKN activity, influencing the balance of intracellular redox state. The RNF41-PRKN pathway regulates autophagosome-lysosome fusion during late mitophagy. Mitophagy is a selective form of autophagy necessary for mitochondrial quality control (PubMed:24949970).

Alternative names

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal NRDP1 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR26281-19

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NRDP1 also known as Neuregulin Receptor Degradation Protein 1 or FLJ11239 acts as an E3 ubiquitin ligase. It is a critical component for targeting certain proteins for ubiquitination and degradation processes. The protein has a molecular weight of approximately 36 kDa. Researchers have observed its expression in various tissues such as the brain heart and liver indicating its diverse role across different organ systems.

Biological function summary

NRDP1 functions in regulating protein levels within cells by tagging them for degradation. It particularly associates with ErbB3 a member of the epidermal growth factor receptor (EGFR) family and induces its ubiquitination. This interaction reflects NRDP1’s role in controlling cell proliferation signaling. It is not part of any multiprotein complex but has shown the capacity to influence individual protein interactions.

Pathways

Researchers identify NRDP1 as a participant in the ubiquitin-proteasome pathway and the ErbB signaling pathway. In the ubiquitin-proteasome pathway it facilitates the degradation of proteins to maintain cellular proteostasis. NRDP1 interacts with Parkin an E3 ubiquitin ligase associated with mitochondrial quality control. In the ErbB signaling pathway it modulates the function of ErbB receptors impacting cellular responses such as growth and differentiation.

Associated diseases and disorders

NRDP1 is implicated in cancer and neurodegenerative diseases. Its dysregulation may lead to improper ErbB3 signaling contributing to the development of certain cancers. Abnormal NRDP1 function correlates with neurodegenerative diseases like Parkinson's disease where it interacts with Parkin. These interactions suggest that NRDP1’s role in proteostasis and cell signaling can influence disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-NRDP1 antibody [EPR26281-19] (ab314642), expandable thumbnail

    Western blot - Anti-NRDP1 antibody [EPR26281-19] (ab314642)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-NRDP1 antibody [EPR26281-19] (ab314642) at 1/1000 dilution

    Lane 1: Human heart tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Rat heart tissue lysate at 20 µg

    Secondary

    Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 1 - 3: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Developed using the ECL technique.

    Observed band size: 37 kDa

    Exposure time: 180s

  • Western blot - Anti-NRDP1 antibody [EPR26281-19] (ab314642), expandable thumbnail

    Western blot - Anti-NRDP1 antibody [EPR26281-19] (ab314642)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Low expression: liver.
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-NRDP1 antibody [EPR26281-19] (ab314642) at 1/1000 dilution

    Lane 1: Human testis tissue lysate at 20 µg

    Lane 2: Human liver tissue lysate at 20 µg

    Lane 3: Mouse testis tissue lysate at 20 µg

    Lane 4: Mouse liver tissue lysate at 20 µg

    Lane 5: Rat testis tissue lysate at 20 µg

    Lane 6: Rat liver tissue lysate at 20 µg

    Secondary

    Lanes 1 - 6: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 1 - 6: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 37 kDa

    Exposure time: 180s

  • Immunoprecipitation - Anti-NRDP1 antibody [EPR26281-19] (ab314642), expandable thumbnail

    Immunoprecipitation - Anti-NRDP1 antibody [EPR26281-19] (ab314642)

    NRDP1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab314642 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314642 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 2: ab314642 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3: Rabbit monoclonal lgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab314642 in HeLa whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-NRDP1 antibody [EPR26281-19] (ab314642) at 1/30 dilution

    All lanes: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Western blot - Anti-NRDP1 antibody [EPR26281-19] (ab314642), expandable thumbnail

    Western blot - Anti-NRDP1 antibody [EPR26281-19] (ab314642)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Lysates/proteins at 20 µg per lane.
    In Western blot, ab314642 was shown to bind specifically to NRDP1. A band was observed at 37 kDa in wild-type Hela cell lysates with whereas no signal observed at this size in NRDP1 knockout cell line Human RNF41 (NRDP1) knockout HeLa cell line ab265213 (knockout cell lysate Human RNF41 (NRDP1) knockout HeLa cell lysate ab263331).
    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-NRDP1 antibody [EPR26281-19] (ab314642) at 1/1000 dilution

    Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: NRDP1 knockout HeLa whole cell lysate at 20 µg

    Lane 3: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 37 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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