Knockout Tested Rabbit Recombinant Monoclonal NRDP1 antibody. Carrier free. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
WB | IP | ICC/IF | Flow Cyt (Intra) | IHC-P | |
---|---|---|---|---|---|
Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
Mouse | Tested | Expected | Not recommended | Not recommended | Not recommended |
Rat | Tested | Expected | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
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Acts as E3 ubiquitin-protein ligase and regulates the degradation of target proteins. Polyubiquitinates MYD88. Negatively regulates MYD88-dependent production of pro-inflammatory cytokines. Can promote TRIF-dependent production of type I interferon and inhibits infection with vesicular stomatitis virus (By similarity). Promotes also activation of TBK1 and IRF3. Involved in the ubiquitination of erythropoietin (EPO) and interleukin-3 (IL-3) receptors. Thus, through maintaining basal levels of cytokine receptors, RNF41 is involved in the control of hematopoietic progenitor cell differentiation into myeloerythroid lineages (By similarity). Contributes to the maintenance of steady-state ERBB3 levels by mediating its growth factor-independent degradation. Involved in the degradation of the inhibitor of apoptosis BIRC6 and thus is an important regulator of cell death by promoting apoptosis. Acts also as a PRKN modifier that accelerates its degradation, resulting in a reduction of PRKN activity, influencing the balance of intracellular redox state. The RNF41-PRKN pathway regulates autophagosome-lysosome fusion during late mitophagy. Mitophagy is a selective form of autophagy necessary for mitochondrial quality control (PubMed:24949970).
FLRF, NRDP1, SBBI03, RNF41, E3 ubiquitin-protein ligase NRDP1, RING finger protein 41, RING-type E3 ubiquitin transferase NRDP1
Knockout Tested Rabbit Recombinant Monoclonal NRDP1 antibody. Carrier free. Suitable for WB, IP and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR26281-19
Affinity purification Protein A
Blue Ice
+4°C
+4°C
ab314643 is the carrier-free version of Anti-NRDP1 antibody [EPR26281-19] ab314642.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
NRDP1 also known as Neuregulin Receptor Degradation Protein 1 or FLJ11239 acts as an E3 ubiquitin ligase. It is a critical component for targeting certain proteins for ubiquitination and degradation processes. The protein has a molecular weight of approximately 36 kDa. Researchers have observed its expression in various tissues such as the brain heart and liver indicating its diverse role across different organ systems.
NRDP1 functions in regulating protein levels within cells by tagging them for degradation. It particularly associates with ErbB3 a member of the epidermal growth factor receptor (EGFR) family and induces its ubiquitination. This interaction reflects NRDP1’s role in controlling cell proliferation signaling. It is not part of any multiprotein complex but has shown the capacity to influence individual protein interactions.
Researchers identify NRDP1 as a participant in the ubiquitin-proteasome pathway and the ErbB signaling pathway. In the ubiquitin-proteasome pathway it facilitates the degradation of proteins to maintain cellular proteostasis. NRDP1 interacts with Parkin an E3 ubiquitin ligase associated with mitochondrial quality control. In the ErbB signaling pathway it modulates the function of ErbB receptors impacting cellular responses such as growth and differentiation.
NRDP1 is implicated in cancer and neurodegenerative diseases. Its dysregulation may lead to improper ErbB3 signaling contributing to the development of certain cancers. Abnormal NRDP1 function correlates with neurodegenerative diseases like Parkinson's disease where it interacts with Parkin. These interactions suggest that NRDP1’s role in proteostasis and cell signaling can influence disease progression.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-NRDP1 antibody [EPR26281-19] ab314642, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-NRDP1 antibody [EPR26281-19] (Anti-NRDP1 antibody [EPR26281-19] ab314642) at 1/1000 dilution
Lane 1: Human heart tissue lysate at 20 µg
Lane 2: Mouse heart tissue lysate at 20 µg
Lane 3: Rat heart tissue lysate at 20 µg
Lanes 1 - 3: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Lanes 1 - 3: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 37 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
This data was developed using Anti-NRDP1 antibody [EPR26281-19] ab314642, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: liver.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-NRDP1 antibody [EPR26281-19] (Anti-NRDP1 antibody [EPR26281-19] ab314642) at 1/1000 dilution
Lane 1: Human testis tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 20 µg
Lane 3: Mouse testis tissue lysate at 20 µg
Lane 4: Mouse liver tissue lysate at 20 µg
Lane 5: Rat testis tissue lysate at 20 µg
Lane 6: Rat liver tissue lysate at 20 µg
Lanes 1 - 6: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Lanes 1 - 6: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Observed band size: 37 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression: liver.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
This data was developed using Anti-NRDP1 antibody [EPR26281-19] ab314642, the same antibody clone in a different buffer formulation.
NRDP1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with Anti-NRDP1 antibody [EPR26281-19] ab314642 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-NRDP1 antibody [EPR26281-19] ab314642 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Anti-NRDP1 antibody [EPR26281-19] ab314642 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal lgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NRDP1 antibody [EPR26281-19] ab314642 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-NRDP1 antibody [EPR26281-19] (Anti-NRDP1 antibody [EPR26281-19] ab314642) at 1/30 dilution
All lanes: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 180s
NRDP1 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with Anti-NRDP1 antibody [EPR26281-19] ab314642 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-NRDP1 antibody [EPR26281-19] ab314642 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2: Anti-NRDP1 antibody [EPR26281-19] ab314642 IP in HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3: Rabbit monoclonal lgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NRDP1 antibody [EPR26281-19] ab314642 in HeLa whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using Anti-NRDP1 antibody [EPR26281-19] ab314642, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
In Western blot, Anti-NRDP1 antibody [EPR26281-19] ab314642 was shown to bind specifically to NRDP1. A band was observed at 37 kDa in wild-type Hela cell lysates with whereas no signal observed at this size in NRDP1 knockout cell line Human RNF41 (NRDP1) knockout HeLa cell line ab265213 (knockout cell lysate Human RNF41 (NRDP1) knockout HeLa cell lysate ab263331).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-NRDP1 antibody [EPR26281-19] (Anti-NRDP1 antibody [EPR26281-19] ab314642) at 1/1000 dilution
Lane 1: Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: NRDP1 knockout HeLa whole cell lysate at 20 µg
Lane 3: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Performed under reducing conditions.
Observed band size: 37 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Lysates/proteins at 20 µg per lane.
In Western blot, Anti-NRDP1 antibody [EPR26281-19] ab314642 was shown to bind specifically to NRDP1. A band was observed at 37 kDa in wild-type Hela cell lysates with whereas no signal observed at this size in NRDP1 knockout cell line Human RNF41 (NRDP1) knockout HeLa cell line ab265213 (knockout cell lysate Human RNF41 (NRDP1) knockout HeLa cell lysate ab263331).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
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