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Mouse Monoclonal NRF1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 33 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NRF1 aa 200-300.


Images

Western blot - Anti-NRF1 antibody [2F9] (AB55744), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [2F9] (AB55744), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human NRF1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q16656

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Alternative names

Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal, NRF1

Recommended products

Mouse Monoclonal NRF1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 33 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NRF1 aa 200-300.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human NRF1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q16656
Clone number
2F9
Purity
Tissue culture supernatant
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The nuclear respiratory factor 1 (NRF1) also known as Alpha-Pal is a transcription factor with an approximate molecular weight of 68 kDa. It plays an important role in the regulation of genes required for mitochondrial DNA transcription and replication. Researchers find NRF1 expression in various tissues including muscle brain and liver. This protein functions mechanistically by binding to specific promoter regions of target genes to control their expression making it essential for maintaining cellular energy metabolism.

Biological function summary

NRF1 influences the expression of genes involved in the mitochondrial function and is part of the transcriptional complex. This complex includes the NRF2 protein where NRF1 partners with co-activators to modulate gene transcription. NRF1 promotes the expression of components essential for mitochondrial biogenesis and cellular respiration which are vital processes for energy production in cells. Therefore NRF1 contributes to maintaining cellular energy homeostasis and overall cellular health.

Pathways

NRF1 integrates into critical regulatory pathways for mitochondrial function and metabolism. NRF1 links closely to the PGC-1α pathway which is instrumental in the regulation of energy metabolism and mitochondrial biogenesis. Additionally NRF1 works in conjunction with NRF2 to regulate oxidative stress responses. These interactions ensure a coordinated cellular response to energy demands and help protect the cell from oxidative damage.

Associated diseases and disorders

NRF1 associates with neurodegenerative diseases and metabolic syndromes. Altered NRF1 regulation can impact diseases such as Alzheimer's disease where it may contribute to neurodegeneration through impaired mitochondrial function. Moreover the dysfunction of NRF1 might relate to metabolic disorders like diabetes as energy metabolism and mitochondrial health are disrupted. NRF1 connects with proteins like SIRT1 in these contexts further implicating NRF1 in these pathological states through its role in energy homeostasis and stress response.

Product promise

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2 product images

  • Western blot - Anti-NRF1 antibody [2F9] (ab55744), expandable thumbnail

    Western blot - Anti-NRF1 antibody [2F9] (ab55744)

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-NRF1 antibody [2F9] (ab55744) at 1 µg/mL

    All lanes: Human skeletal muscle lysate

    Secondary

    All lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate

    Predicted band size: 54 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [2F9] (ab55744), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [2F9] (ab55744)

    NRF1 antibody (ab55744) used in immunohistochemistry at μg/ml on formalin fixed and paraffin embedded human kidney.

    This image was generated using the ascites version of the product.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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