Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)

Rabbit Recombinant Monoclonal NRF1 antibody. Carrier free. Suitable for ChIC/CUT&RUN-seq, IHC-P, IP, ChIP, WB, ICC/IF, ChIP-seq, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

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ChIP - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (AB221792), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ChIC/CUT&RUN-seq	IHC-P	IP	ChIP	WB	ICC/IF	ChIP-seq	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Expected	Tested	Tested	Tested
Mouse	Predicted	Predicted	Predicted	Predicted	Expected	Predicted	Predicted	Predicted
Rat	Predicted	Predicted	Predicted	Predicted	Expected	Predicted	Predicted	Predicted

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 8 µg for 10⁷ Cells	Notes -

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes ab199376- Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

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2 products for Alternative Version

Target data

See full target information NRF1

Function

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Alternative names

Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal, NRF1

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR5554(N)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab221792 is the carrier-free version of Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The nuclear respiratory factor 1 (NRF1) also known as Alpha-Pal is a transcription factor with an approximate molecular weight of 68 kDa. It plays an important role in the regulation of genes required for mitochondrial DNA transcription and replication. Researchers find NRF1 expression in various tissues including muscle brain and liver. This protein functions mechanistically by binding to specific promoter regions of target genes to control their expression making it essential for maintaining cellular energy metabolism.

Biological function summary

NRF1 influences the expression of genes involved in the mitochondrial function and is part of the transcriptional complex. This complex includes the NRF2 protein where NRF1 partners with co-activators to modulate gene transcription. NRF1 promotes the expression of components essential for mitochondrial biogenesis and cellular respiration which are vital processes for energy production in cells. Therefore NRF1 contributes to maintaining cellular energy homeostasis and overall cellular health.

Pathways

NRF1 integrates into critical regulatory pathways for mitochondrial function and metabolism. NRF1 links closely to the PGC-1α pathway which is instrumental in the regulation of energy metabolism and mitochondrial biogenesis. Additionally NRF1 works in conjunction with NRF2 to regulate oxidative stress responses. These interactions ensure a coordinated cellular response to energy demands and help protect the cell from oxidative damage.

Associated diseases and disorders

NRF1 associates with neurodegenerative diseases and metabolic syndromes. Altered NRF1 regulation can impact diseases such as Alzheimer's disease where it may contribute to neurodegeneration through impaired mitochondrial function. Moreover the dysfunction of NRF1 might relate to metabolic disorders like diabetes as energy metabolism and mitochondrial health are disrupted. NRF1 connects with proteins like SIRT1 in these contexts further implicating NRF1 in these pathological states through its role in energy homeostasis and stress response.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

13 product images

ChIP - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)
Chromatin was prepared from Hela cells according to the Abcam Dual X-ChIP protocol. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
The ChIP was performed with 25 μg of chromatin, 5 μg of Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 (red), and 20 μl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
Primers and probes are located in the first kb of the transcribed region.
*http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932).
ChIP - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)
Chromatin was prepared from NIH/3T3 treated with MG-132(2uM 16h) cells according to the Abcam Dual X-ChIP protocol*. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
The ChIP was performed with 25 μg of chromatin, 5 μg of Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 (red), and 20 μl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
Primers and probes are located in the first kb of the transcribed region.
*http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932).
ChIP-sequencing - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)
Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 8 μg of Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 [EPR5554(N)]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.
Additional screenshots of mapped reads can be downloaded here.
Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)
Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 (purified) at a dilution of 1/50 immunoprecipitating NRF1 in 293T whole cell lysate.
Lane 1 (input): 293T whole cell lysate (10μg)
Lane 2 (+): Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 + 293T whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 in 293T whole cell lysate.
For western blotting, VeriBlot for IP Detection Reagent (HRP) ab131366 VeriBlot for IP (HRP) was used for detection at 1/1000 dilution.
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932).
All lanes: Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932)
Predicted band size: 54 kDa
Observed band size: 68 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cevical carcinoma tissue labelling NRF1 with purified Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 at a dilution of 1/100. Heat mediated antigen retrieval was performed using EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932).
Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)
Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling NRF1 with purified Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932).
Flow Cytometry (Intracellular) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)
Intracellular Flow Cytometry analysis of 293T cells labelling NRF1 with purified Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 at a dilution of 1/150 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932).
Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)
Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 (unpurified) at a dilution of 1/10 immunoprecipitating NRF1 in 293T cell lysate.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932).
All lanes: Immunoprecipitation - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932)
Predicted band size: 54 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labeling NRF1 with unpurified Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 at a dilution of 1/50.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932).
Flow Cytometry (Intracellular) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)
Intracellular flow cytometric analysis of permeabilized 293T cells labeling NRF1 with unpurified Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 at a dilution of 1/10 (red) compared to a negative control (rabbit IgG, green).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labeling NRF1 with unpurified Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 at a dilution of 1/50.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric adenocarcinoma tissue labeling NRF1 with unpurified Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 at a dilution of 1/50.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ChIC/CUT&RUN sequencing - Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade - BSA and Azide free (ab221792)
ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 5 µg of Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932 [EPR5554(N)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown. The ChIP data was conducted on chromatin prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 10^7 HeLa cells and 8 µg of Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads.

Additional screenshots of mapped reads can be found in the Protocol booklet in the Support and downloads section.

The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-NRF1 antibody [EPR5554(N)] - ChIP Grade ab175932).