Anti-Nrf2 antibody ab137550 is a rabbit polyclonal antibody that is used in Nrf2 western blotting. Suitable for human samples.
- Specificity confirmed with NFE2L2 knockout cell line validation
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- Toxins 15:2023Quercetin Attenuates the Combined Effects of Zearalenone and Lipopolysaccharide on IPEC-J2 Cell Injury through Activating the Nrf2 Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuanqi Wang,Yurong Fu,Ruqi Wang,Qiyuan Wang,Hao Yu,Jing ZhangPubMed 38133183
- PeerJ 11:e164512023Lymphoid-specific helicase inhibits cervical cancer cells ferroptosis by promoting Nrf2 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiwei Tie,Fenfen GePubMed 38047020
- Heliyon 9:e218892023Sugarcane leaf polysaccharide exerts a therapeutic effect on cardiovascular diseases through necroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKaili Sun,Renyikun Yuan,Jia He,Youqiong Zhuo,Ming Yang,Erwei Hao,Xiaotao Hou,Chun Yao,Shilin Yang,Hongwei GaoPubMed 38027563
- Heliyon 9:e214122023Mechanism of Nrf2/miR338-3p/TRAP-1 pathway involved in hyperactivation of synovial fibroblasts in patients with osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Jie,Ya Wu,Changzhi Song,Yi Cheng,Yunfei Liu,Kang ChenPubMed 37920489
- Scientific reports 13:162052023The bisphosphonates alendronate and zoledronate induce adaptations of aerobic metabolism in permanent human endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrianna Budzinska,Lukasz Galganski,Wieslawa JarmuszkiewiczPubMed 37758809
- Materials today. Bio 23:1008112023Melatonin-loaded self-healing hydrogel targets mitochondrial energy metabolism and promotes annulus fibrosus regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiayu Hu,Xin Tian,Chunju Yang,Feng Ling,Hao Liu,Xuesong Zhu,Ming Pei,Huilin Yang,Tao Liu,Yong Xu,Fan HePubMed 37810753
- Cells 12:2023Transcription Factor NFE2L1 Decreases in Glomerulonephropathies after Podocyte Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMustafa Elshani,In Hwa Um,Steve Leung,Paul A Reynolds,Alex Chapman,Mary Kudsy,David J HarrisonPubMed 37681897
- International journal of chronic obstructive pulmonary disease 18:1883-18972023Ginseng Saponin Rb1 Attenuates Cigarette Smoke Exposure-Induced Inflammation, Apoptosis and Oxidative Stress via Activating Nrf2 and Inhibiting NF-κB Signaling Pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhizheng Li,Li Li,Xiaohui Lv,Yingqian Hu,Kun CuiPubMed 37662486
- Aging cell 22:e139582023Inhibition of abnormal C/EBPβ/α-Syn signaling pathway through activation of Nrf2 ameliorates Parkinson's disease-like pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZefang Lin,Lixuan Huang,Qianqian Cao,Hanyue Luo,Wei Yao,Ji-Chun ZhangPubMed 37614147
- Antioxidants (Basel, Switzerland) 12:2023Carvedilol Phenocopies PGC-1α Overexpression to Alleviate Oxidative Stress, Mitochondrial Dysfunction and Prevent Doxorubicin-Induced Toxicity in Human iPSC-Derived Cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNnamdi Uche,Qiang Dai,Shuping Lai,Kurt Kolander,Mai Thao,Elizabeth Schibly,Xavier Sendaydiego,Jacek Zielonka,Ivor J BenjaminPubMed 37627583
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NFE2L2 aa 100-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q16236
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Reactivity data
Select an application| IHC-P | IP | ChIP | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Tested | Not recommended |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
ChIP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Assay dependent |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes Detects a band of approximately 110 kDa (predicted molecular weight: 68 kDa). NRF2 is bound to ubiquitin when it is the nucleus so MW would be higher than cytoplasm. Stimuli treated samples are recommended. Expression levels may be better for nuclear fractions or for cell lines treated with MG132. |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription factor that plays a key role in the response to oxidative stress: binds to antioxidant response (ARE) elements present in the promoter region of many cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression, thereby neutralizing reactive electrophiles (PubMed:11035812, PubMed:19489739, PubMed:29018201, PubMed:31398338). In normal conditions, ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex (PubMed:11035812, PubMed:15601839, PubMed:29018201). In response to oxidative stress, electrophile metabolites inhibit activity of the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2, heterodimerization with one of the small Maf proteins and binding to ARE elements of cytoprotective target genes (PubMed:19489739, PubMed:29590092). The NFE2L2/NRF2 pathway is also activated in response to selective autophagy: autophagy promotes interaction between KEAP1 and SQSTM1/p62 and subsequent inactivation of the BCR(KEAP1) complex, leading to NFE2L2/NRF2 nuclear accumulation and expression of cytoprotective genes (PubMed:20452972). May also be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region (PubMed:7937919).
Alternative names
Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, Nrf-2, HEBP1, NRF2, NFE2L2
Recommended products
Anti-Nrf2 antibody ab137550 is a rabbit polyclonal antibody that is used in Nrf2 western blotting. Suitable for human samples.
- Specificity confirmed with NFE2L2 knockout cell line validation
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NFE2L2 aa 100-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q16236
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
NRF2 is bound to ubiquitin when it is the nucleus so MW would be higher than cytoplasm.
Please note:
Nrf2 antibody (ab137550) detects no signal in most untreated samples for WB. Stimuli treated samples are recommended. Nrf2 expression is stimulated by oxidative stress, electrophiles and chemical activators (PMID: 25761198, PMID: 27638861 and PMID: 28587109).
WB protocol info included in legend for MDA-MB-231 nuclear extract treated with 30 μM tBHQ for 4 hours (+).
Abcam recommended secondaries - Goat Anti-Rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab205718) and Goat Anti-Rabbit Alexa Fluor®488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Or search our wide range of secondary antibodies for use with your experiment.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nrf2 also known as nuclear factor erythroid 2-related factor 2 is a transcription factor with a molecular weight of approximately 66 kDa. It plays a mechanical role in regulating the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. Nrf2 is ubiquitously expressed in various tissues including the liver lungs and skin. Nrf2 activation occurs when it translocates from the cytoplasm to the nucleus to bind the antioxidant response element (ARE) in the DNA starting transcription of target genes.
- Biological function summary
Nrf2 acts as an important regulator of the cellular antioxidant response. It works in conjunction with its partner protein Keap1 forming a complex that controls its stability and degradation. Under normal conditions Keap1 keeps Nrf2 in the cytoplasm where it is targeted for ubiquitination and degradation. Once activated by oxidative stress or electrophiles Nrf2 dissociates from Keap1 thereby avoiding degradation and relocates to the nucleus to activate the transcription of ARE-dependent genes. This activity boosts the cellular response to oxidative stress by inducing genes involved in detoxification and cellular defense.
- Pathways
Nrf2 plays a significant role in the oxidative stress response and detoxification pathways. Nrf2 activation is linked closely to the PI3K/Akt signaling pathway which influences cell survival growth and metabolism. This pathway also interacts with other important proteins like GSK-3β which can modulate Nrf2 activity and stability. Through these pathways Nrf2 orchestrates a defense mechanism against reactive oxygen species (ROS) by boosting the expression of antioxidant enzymes and detoxifying proteins.
- Associated diseases and disorders
Nrf2 has been associated with conditions like cancer and neurodegenerative diseases. In cancer aberrant Nrf2 activation may lead to enhanced tumor survival by increasing expression of cytoprotective genes which makes cancer cells resistant to chemotherapy. Nrf2 also interacts with proteins such as p53 which play roles in tumor suppression and cellular stress responses. In neurodegenerative disorders reduced Nrf2 activity can contribute to oxidative stress leading to neuron damage and disease progression with proteins like amyloid-beta also being linked with oxidative processes affected by Nrf2 functionality.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
ChIP - Anti-Nrf2 antibody (ab137550)
ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or Anti-Nrf2 antibody (ab137550). The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.
Western blot - Anti-Nrf2 antibody (ab137550)
The observed MW (110kDa) is based on PMID: 22703241
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE
Detection: Trident ECL plus-Enhanced.
All lanes: Western blot - Anti-Nrf2 antibody (ab137550) at 1/500 dilution
Lane 1: Untreated HepG2 whole cell extracts at 30 µg
Lane 2: Treated HepG2 whole cell extracts at 30 µg
SecondaryAll lanes: A HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 68 kDa
Observed band size: 110 kDa
Immunoprecipitation - Anti-Nrf2 antibody (ab137550)
ab137550 at 5 μg immunoprecipitating Nrf2 in HepG2 whole cell extracts.
Lane 1 (-): Ctrl IgG instead of ab137550 in HepG2 whole cell extracts.
Lane 2 (+): ab137550 + HepG2 whole cell extracts.For western blotting an anti-Rabbit IgG was used as a secondary reagent.
All lanes: Immunoprecipitation - Anti-Nrf2 antibody (ab137550)
Predicted band size: 68 kDa
Western blot - Anti-Nrf2 antibody (ab137550)
7.5% gel.
Running condition: 80V, 15min; 140V, 40min.
Transfer condition:Semi-dry, 18 V, 60min (Nitrocellulose membrane)
Blocking condition: 5% non-fat milk in TBST, RT, 60min.
Primary antibody incubation: 4°C overnight.
Secondary antibody incubation: Room temperature for 1 hour.
Washing condition: 5 ml TBST, 4 x 5min.
ECL detection.
The observed MW (110kDa) is based on PMID: 22703241
All lanes: Western blot - Anti-Nrf2 antibody (ab137550) at 1/1000 dilution
Lane 1: Untreated (-) MDA-MB-231 nuclear extract at 30 µg
Lane 2: MDA-MB-231 nuclear extract treated with 30 μM tBHQ for 4 hours (+) at 30 µg
SecondaryAll lanes: Rabbit IgG antibody (HRP) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDa
Observed band size: 110 kDa
Western blot - Anti-Nrf2 antibody (ab137550)
5% SDS-PAGE
The observed MW (110kDa) is based on PMID: 22703241
All lanes: Western blot - Anti-Nrf2 antibody (ab137550) at 1/1000 dilution
Lane 1: Non-transfected (-) HEK-293T whole cell extracts at 30 µg
Lane 2: NRF2-transfected (+, including 3xFlag-tag) HEK-293T whole cell extracts at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 68 kDa
Observed band size: 110 kDa
Western blot - Anti-Nrf2 antibody (ab137550)
Western blot: Anti-NFE2L2 antibody (ab137550) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab137550 was shown to bind specifically to NFE2L2. A band was observed at 85 kDa in wild-type A549 cell lysates with no signal observed at this size in NFE2L2 knockout cell line Human NFE2L2 knockout A549 cell line[21] ab285359 (knockout cell lysate ab289682). To generate this image, wild-type and NFE2L2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-Nrf2 antibody (ab137550) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: NFE2L2 knockout A549 cell lysate at 20 µg
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: Huh7 cell lysate at 20 µg
Predicted band size: 68 kDa
Observed band size: 85 kDa
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