JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB326605

Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Nrf2 antibody. Carrier free. Suitable for Dot, ChIP-seq, IP, Flow Cyt (Intra), ICC/IF, WB and reacts with Transfected cell lysate - Mouse, Human, Mouse, Rat samples.

View Alternative Names

Nrf2, Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2

19 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) HeLa cells treated with MG-132 (10 μM) for 4 hr cells labelling Nrf2 with ab325240 at 1/50 (10.22 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing increased nuclear staining in HeLa cells (shown in green) treated with MG-132 (10 μM) for 4 hr. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervical adenocarcinoma epithelial cell) treated with 10uM MG-132 for 4 hours (Green) and Untreated control (Magenta) cells labelling Nrf2 with ab325240 at 1/500 dilution (0.1ug) / Green and Red compared with a Rabbit monoclonal IgG (ab172730) (Black) and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

ChIP-sequencing - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 106 cells and 4 µg of ab325240 [EPR30100-592]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

ChIP-sequencing - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 106 cells and 4 µg of ab325240 [EPR30100-592]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

ChIP-sequencing - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Chromatin was prepared from HeLa cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 106 cells and 4 µg of ab325240 [EPR30100-592]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

Immunoprecipitation - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • IP

Supplier Data

Immunoprecipitation - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Nrf2 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab325240 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325240 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

To minimize protein degradation cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-Nrf2 antibody [EPR30100-592] (<a href='/en-us/products/primary-antibodies/nrf2-antibody-epr30100-592-ab325240'>ab325240</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 25 µg

Lane 2:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/nrf2-antibody-epr30100-592-ab325240'>ab325240</a> in HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 105 kDa

false

Exposure time: 50s

Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell) Hepa1-6 cells (shown in green) treated with Diethylmaleate (100 μM) for 4 hr cells labelling Nrf2 with ab325240 at 1/50 (10.22 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing increased nuclear staining in Hepa1-6 cells (shown in green) treated with Diethylmaleate (100 μM) for 4 hr. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized C6 (rat glial tumor glial cell) C6 cells treated with MG-132 (10 μM) for 16 hr cells labelling Nrf2 with ab325240 at 1/50 (10.22 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing increased nuclear staining in C6 cells (shown in green) treated with MG-132 (10 μM) for 16 hr. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 1ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Flow Cytometry (Intracellular) - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Hepa1-6 (mouse hepatoma epithelial cell) treated with 10uM MG-132 for 4 hours (Green) and Untreated control (Magenta) cells labelling Nrf2 with ab325240 at 1/500 dilution (0.1ug) / Green and Red compared with a Rabbit monoclonal IgG (ab172730) (Black) and Grey isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Immunoprecipitation - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • IP

Supplier Data

Immunoprecipitation - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Nrf2 was immunoprecipitated from 0.35 mg C6 (rat glial tumor glial cell) whole cell lysate with ab325240 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325240 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

To minimize protein degradation cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-Nrf2 antibody [EPR30100-592] (<a href='/en-us/products/primary-antibodies/nrf2-antibody-epr30100-592-ab325240'>ab325240</a>) at 1/30 dilution

Lane 1:

C6 (rat glial tumor glial cell) whole cell lysate at 15 µg

Lane 2:

C6 (rat glial tumor glial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/nrf2-antibody-epr30100-592-ab325240'>ab325240</a> in C6 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 105 kDa

false

Exposure time: 145s

ChIP-sequencing - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Chromatin was prepared from Hepa1-6 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 106 cells and 4 µg of ab325240 [EPR30100-592]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

ChIP-sequencing - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Chromatin was prepared from Hepa1-6 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 106 cells and 4 µg of ab325240 [EPR30100-592]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

Immunoprecipitation - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • IP

Supplier Data

Immunoprecipitation - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Nrf2 was immunoprecipitated from 0.35 mg Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate with ab325240 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325240 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

To minimize protein degradation cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-Nrf2 antibody [EPR30100-592] (<a href='/en-us/products/primary-antibodies/nrf2-antibody-epr30100-592-ab325240'>ab325240</a>) at 1/1000 dilution

Lane 1:

Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 18 µg

Lane 2:

Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/nrf2-antibody-epr30100-592-ab325240'>ab325240</a> in Hepa1-6 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 105 kDa

false

Exposure time: 180s

ChIP-sequencing - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • ChIP-seq

Lab

ChIP-sequencing - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Chromatin was prepared from Hepa1-6 cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 5 x 106 cells and 4 µg of ab325240 [EPR30100-592]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The Input control is also shown.

Western blot - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • WB

Supplier Data

Western blot - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 3600148; PMID : 22932898; PMID : 38495294).

The expression of is upregulated in response to MG-132 treatment (PMID : 22932898).

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Nrf2 antibody [EPR30100-592] (<a href='/en-us/products/primary-antibodies/nrf2-antibody-epr30100-592-ab325240'>ab325240</a>) at 1/1000 dilution

Lane 1:

Untreated U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

U-2 OS treated with 10uM MG-132 for 4 hours whole cell lysate at 20 µg

Lane 3:

Untreated MEF (mouse embryo fibroblast) whole cell lysate at 20 µg

Lane 4:

MEF treated with 20uM MG-132 for 4 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 105 kDa,36 kDa

false

Exposure time: 15s

Western blot - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • WB

Supplier Data

Western blot - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 3600148; PMID : 22932898; PMID : 38495294).

To minimize protein degradation cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Nrf2 antibody [EPR30100-592] (<a href='/en-us/products/primary-antibodies/nrf2-antibody-epr30100-592-ab325240'>ab325240</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 105 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • WB

Supplier Data

Western blot - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 3600148; PMID : 22932898; PMID : 38495294).

The expression of Nrf2 is upregulated in response to MG-132 treatment (PMID : 22932898; PMID : 38495294).

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Nrf2 antibody [EPR30100-592] (<a href='/en-us/products/primary-antibodies/nrf2-antibody-epr30100-592-ab325240'>ab325240</a>) at 1/1000 dilution

Lane 1:

Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa treated with 10&micro;M MG-132 for 4 hours whole cell lysate at 20 µg

Lane 3:

Untreated Hepa1-6 (mouse hepatoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Hepa1-6 treated with 100&micro;M Diethylmaleate for 4 hours whole cell lysate at 20 µg

Lane 5:

Untreated C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Lane 6:

C6 (rat glial tumor glial cell) treated with 10&micro;M MG-132 for 16 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 105 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • WB

Supplier Data

Western blot - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 3600148; PMID : 22932898; PMID : 38495294).

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Nrf2 antibody [EPR30100-592] (<a href='/en-us/products/primary-antibodies/nrf2-antibody-epr30100-592-ab325240'>ab325240</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting Nrf2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 105 kDa,36 kDa

false

Exposure time: 180s

Dot Blot - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)
  • Dot

Supplier Data

Dot Blot - Anti-Nrf2 antibody [EPR30100-592] - BSA and Azide free (AB326605)

This data was developed using ab325240, the same antibody clone in a different buffer formulation.

Dot blot analysis of Nrf2 using ab325240 at 1 : 1000 (0.511 ug/ml) followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1 : 100000 dilution.

Lane1 : 293T cells transfected with a mouse Nrf2 expression vector containing a myc-His-tag® whole cell lysate
Lane2 : 293T cells transfected with a mouse NF2L1 expression vector containing a myc-His-tag® whole cell lysate
Lane3 : 293T cells transfected with a mouse NF2L3 expression vector containing a myc-His-tag® whole cell lysate

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with mouse NF2L1 and NF2L3

In Dot Blot Anti-6X His tag® antibody [AD1.1.10] (ab15149) staining at 1/1000 dilution.

All lanes:

Dot Blot - Anti-Nrf2 antibody [EPR30100-592] (<a href='/en-us/products/primary-antibodies/nrf2-antibody-epr30100-592-ab325240'>ab325240</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with a mouse Nrf2 expression vector containing a myc-His-tag®, whole cell lysate

Lane 2:

293T cells transfected with a mouse NF2L1 expression vector containing a myc-His-tag®, whole cell lysate

Lane 3:

293T cells transfected with a mouse NF2L3 expression vector containing a myc-His-tag®, whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30100-592

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human, Rat

Applications

Flow Cyt (Intra), ChIP-seq, IP, Dot, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "ChIPseq" : {"fullname" : "ChIP-sequencing", "shortname":"ChIP-seq"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ChIPseq-species-checked": "testedAndGuaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "ChIPseq-species-checked": "guaranteed", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Transfected cell lysate - Mouse": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "ChIPseq-species-checked": "notRecommended", "ChIPseq-species-dilution-info": "", "ChIPseq-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab326605 is the carrier-free version of ab325240

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor that plays a key role in the response to oxidative stress : binds to antioxidant response (ARE) elements present in the promoter region of many cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression, thereby neutralizing reactive electrophiles (PubMed : 12032331, PubMed : 14517290, PubMed : 14517554, PubMed : 31398338, PubMed : 9240432, PubMed : 9887101). In normal conditions, ubiquitinated and degraded in the cytoplasm by the BCR(KEAP1) complex (PubMed : 14517290, PubMed : 15282312, PubMed : 15367669, PubMed : 15581590). In response to oxidative stress, electrophile metabolites inhibit activity of the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2, heterodimerization with one of the small Maf proteins and binding to ARE elements of cytoprotective target genes (PubMed : 12032331). The NFE2L2/NRF2 pathway is also activated in response to selective autophagy : autophagy promotes interaction between KEAP1 and SQSTM1/p62 and subsequent inactivation of the BCR(KEAP1) complex, leading to NFE2L2/NRF2 nuclear accumulation and expression of cytoprotective genes (PubMed : 20173742, PubMed : 20421418). The NFE2L2/NRF2 pathway is also activated during the unfolded protein response (UPR), contributing to redox homeostasis and cell survival following endoplasmic reticulum stress (PubMed : 14517290, PubMed : 14978030). May also be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region (By similarity). Also plays an important role in the regulation of the innate immune response. It is a critical regulator of the innate immune response and survival during sepsis by maintaining redox homeostasis and restraint of the dysregulation of pro-inflammatory signaling pathways like MyD88-dependent and -independent and TNF signaling (PubMed : 16585964). Suppresses macrophage inflammatory response by blocking pro-inflammatory cytokine transcription and the induction of IL6 (PubMed : 27211851). Binds to the proximity of pro-inflammatory genes in macrophages and inhibits RNA Pol II recruitment. The inhibition is independent of the Nrf2-binding motif and reactive oxygen species level (PubMed : 27211851). Represses antiviral cytosolic DNA sensing by suppressing the expression of the adapter protein STING1 and decreasing responsiveness to STING1 agonists while increasing susceptibility to infection with DNA viruses (By similarity).
See full target information Nfe2l2
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com