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AB153908

Anti-NRG1 antibody

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(1 Publication)

Rabbit Polyclonal NRG1 antibody. Suitable for ICC/IF, WB and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NRG1 aa 1-250.

View Alternative Names

GGF, HGL, HRGA, NDF, SMDF, NRG1, Pro-NRG1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-NRG1 antibody (AB153908)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NRG1 antibody (AB153908)

Immunofluorescence analysis of SK N SH cells fixed in 4% paraformaldehyde at RT for 15 min labelling Neuregulin 1 protein at nucleus using ab153908 at a 1/500 dilution (Green). Phalloidin, a cytoskeleton marker, was stained at a 1/100 dilution (Red). Hoechst 33342 was used for nuclear staining (Blue).

Western blot - Anti-NRG1 antibody (AB153908)
  • WB

Supplier Data

Western blot - Anti-NRG1 antibody (AB153908)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-NRG1 antibody (ab153908) at 1/1000 dilution

Lane 1:

U87 MG whole cell extract at 30 µg

Lane 2:

U87 MG whole cell extract with conditioned medium at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 70 kDa

false

Western blot - Anti-NRG1 antibody (AB153908)
  • WB

Supplier Data

Western blot - Anti-NRG1 antibody (AB153908)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-NRG1 antibody (ab153908) at 1/1000 dilution

All lanes:

Human brain tissue lysates at 30 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 70 kDa

false

Western blot - Anti-NRG1 antibody (AB153908)
  • WB

Unknown

Western blot - Anti-NRG1 antibody (AB153908)

7.5% SDS PAGE

All lanes:

Western blot - Anti-NRG1 antibody (ab153908) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 70 kDa

false

Western blot - Anti-NRG1 antibody (AB153908)
  • WB

Supplier Data

Western blot - Anti-NRG1 antibody (AB153908)

Samples were separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-NRG1 antibody (ab153908) at 1/1000 dilution

All lanes:

Rat brain tissue lysates at 50 µg

Secondary

All lanes:

HRP conjugated anti rabbit IgG antibody

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human, Rat

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human NRG1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q02297

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuregulin 1 (NRG1) also known as heregulin beta 1 is a member of the neuregulin protein family. It is a signaling protein with a molecular mass that typically ranges around 44 kDa. Researchers have found NRG1 expressed in various tissues including the heart brain and certain epithelial cells. It plays important roles in cell-cell communication by binding to ErbB family receptors particularly ErbB3 and ErbB4 which trigger downstream signaling processes.
Biological function summary

NRG1 is involved in several cellular activities such as cell growth survival and differentiation. It is not part of a complex but acts directly through its interactions with receptor tyrosine kinases in the ErbB receptor family. NRG1 influences cardiac development and neural function by mediating these cellular processes promoting the development and maintenance of neural and cardiac tissues.

Pathways

NRG1 is involved in the PI3K-Akt and MAPK/ERK signaling pathways. These pathways are critical for cellular growth differentiation and survival supporting the functions of NRG1 in various tissues. The interaction with ErbB2 and ErbB3 receptors is especially significant for these pathways as they help regulate the cellular responses initiated by NRG1 signaling.

NRG1 plays an impactful role in the development of certain types of cancer and schizophrenia. Dysregulation or mutations in the NRG1 gene can lead to altered signaling pathways which are connected to the pathogenesis of these conditions. In cancer the interaction with ErbB2 has been under investigation as it may promote tumorigenesis. In the context of schizophrenia NRG1's effects on neural development and neurotransmission are linked with the disorder's symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4 (PubMed : 10867024, PubMed : 7902537). Binds to ERBB3 (PubMed : 20682778). Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV : ITGB3 or ITGA6 : ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed : 20682778). Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons (By similarity).
See full target information NRG1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e70140 PubMed39434203

2024

Hoxc10-mediated 'positional memory' regulates cartilage formation subsequent to femoral heterotopic grafting.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyue Song,Yujia Hao,Qingpeng Xie,Xiaohang Chen,Na Li,Jia Wang,Xiaoxuan Zhang,Yuan Zhang,Jinjia Hong,Shuyun Xue,Pengfei Zhang,Si Xie,Xing Wang
View all publications
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