Rabbit Polyclonal NRG1 antibody. C-terminal. Suitable for IHC-P, ICC, WB, IHC-Fr and reacts with Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human NRG1 aa 600 to C-terminus.
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- Experimental and therapeutic medicine 26:4682023Effect of thymosin β4 on lipopolysaccharide‑stimulated brain microvascular endothelial cell remodeling: A possible role in blood‑brain barrier injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWilliam Stewart,Christina Hejl,Rakeshwar S Guleria,Sudhiranjan GuptaPubMed 37664684
- Disease models & mechanisms 16:2023Fibroblast-derived EGF ligand neuregulin 1 induces fetal-like reprogramming of the intestinal epithelium without supporting tumorigenic growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesToni T Lemmetyinen,Emma W Viitala,Linnea Wartiovaara,Tuomas Kaprio,Jaana Hagström,Caj Haglund,Pekka Katajisto,Timothy C Wang,Eva Domènech-Moreno,Saara OllilaPubMed 36912192
- Nature communications 14:15592023Cellular mechanisms of heterogeneity in NF2-mutant schwannoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChristine Chiasson-MacKenzie,Jeremie Vitte,Ching-Hui Liu,Emily A Wright,Elizabeth A Flynn,Shannon L Stott,Marco Giovannini,Andrea I McClatcheyPubMed 36944680
- Science advances 9:eadd89772023Single-cell dissection of cellular and molecular features underlying human cervical squamous cell carcinoma initiation and progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Liu,Min Zhang,Xinlong Yan,Yanli Ni,Yandong Gong,Cong Wang,Xiaoling Zhang,Lingfei Wan,Hui Yang,Chen Ge,Yunqiao Li,Wenxue Zou,Rui Huang,Xiaohui Li,Bing Sun,Bing Liu,Jinbo Yue,Jinming YuPubMed 36706185
- Journal of integrative neuroscience 20:613-6222021Prenatal sevoflurane exposure causes abnormal development of the entorhinal cortex in rat offspring.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Gao,Tianyun Zhao,Yanxin Chen,Zhixiang Sun,Junming Lu,Ziwen Shi,Xingrong SongPubMed 34645094
- Cell stem cell 27:646-662.e72020Mesenchymal Niche-Derived Neuregulin-1 Drives Intestinal Stem Cell Proliferation and Regeneration of Damaged Epithelium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThierry Jardé,Wing Hei Chan,Fernando J Rossello,Tanvir Kaur Kahlon,Mandy Theocharous,Teni Kurian Arackal,Tracey Flores,Mégane Giraud,Elizabeth Richards,Eva Chan,Genevieve Kerr,Rebekah M Engel,Mirsada Prasko,Jacqueline F Donoghue,Shin-Ichi Abe,Toby J Phesse,Christian M Nefzger,Paul J McMurrick,David R Powell,Roger J Daly,Jose M Polo,Helen E AbudPubMed 32693086
- PloS one 14:e02165272019The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrank L Rice et. al.PubMed 31107888
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human NRG1 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q02297
Reactivity data
Select an application| IHC-P | ICC | WB | IHC-Fr | |
|---|---|---|---|---|
| Human | Expected | Tested | Expected | Expected |
| Mouse | Predicted | Predicted | Predicted | Predicted |
| Rat | Tested | Expected | Tested | Tested |
| Chicken | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info - | Notes - |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-1.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.10000-0.50000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.50000-1.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Chicken | Dilution info - | Notes - |
Associated Products
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Target data
Function
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4 (PubMed:10867024, PubMed:7902537). Binds to ERBB3 (PubMed:20682778). Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:20682778). Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons (By similarity).
Alternative names
GGF, HGL, HRGA, NDF, SMDF, NRG1, Pro-NRG1
Recommended products
Rabbit Polyclonal NRG1 antibody. C-terminal. Suitable for IHC-P, ICC, WB, IHC-Fr and reacts with Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human NRG1 aa 600 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human NRG1 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q02297
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Neuregulin 1 (NRG1) also known as heregulin beta 1 is a member of the neuregulin protein family. It is a signaling protein with a molecular mass that typically ranges around 44 kDa. Researchers have found NRG1 expressed in various tissues including the heart brain and certain epithelial cells. It plays important roles in cell-cell communication by binding to ErbB family receptors particularly ErbB3 and ErbB4 which trigger downstream signaling processes.
- Biological function summary
NRG1 is involved in several cellular activities such as cell growth survival and differentiation. It is not part of a complex but acts directly through its interactions with receptor tyrosine kinases in the ErbB receptor family. NRG1 influences cardiac development and neural function by mediating these cellular processes promoting the development and maintenance of neural and cardiac tissues.
- Pathways
NRG1 is involved in the PI3K-Akt and MAPK/ERK signaling pathways. These pathways are critical for cellular growth differentiation and survival supporting the functions of NRG1 in various tissues. The interaction with ErbB2 and ErbB3 receptors is especially significant for these pathways as they help regulate the cellular responses initiated by NRG1 signaling.
- Associated diseases and disorders
NRG1 plays an impactful role in the development of certain types of cancer and schizophrenia. Dysregulation or mutations in the NRG1 gene can lead to altered signaling pathways which are connected to the pathogenesis of these conditions. In cancer the interaction with ErbB2 has been under investigation as it may promote tumorigenesis. In the context of schizophrenia NRG1's effects on neural development and neurotransmission are linked with the disorder's symptoms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunocytochemistry - Anti-NRG1 antibody - C-terminal (ab191139)
Immunocytochemical analysis of HeLa cells labeling NRG1 with ab191139 at 1 μg/ml.
Western blot - Anti-NRG1 antibody - C-terminal (ab191139)
All lanes: Western blot - Anti-NRG1 antibody - C-terminal (ab191139) at 0.5 µg/mL
Lane 1: Rat Spleen Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: Rat Brain Tissue Lysate
Lane 4: HeLa Cell Lysate
Lane 5: SMMC Cell Lysate
Predicted band size: 70 kDa
Immunohistochemistry (Frozen sections) - Anti-NRG1 antibody - C-terminal (ab191139)
Immunohistochemical analysis of frozen Rat Intestine Tissue labeling NRG1 with ab191139 at 1 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRG1 antibody - C-terminal (ab191139)
Immunohistochemical analysis of paraffin-embedded Rat Intestine Cancer Tissue labeling NRG1 with ab191139 at 1 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NRG1 antibody - C-terminal (ab191139)
Immunohistochemical analysis of paraffin-embedded Rat Intestine Tissue labeling NRG1 with ab191139 at 1 μg/ml.
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