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AB281228

Anti-NRG1 antibody [EPR23075-57] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal NRG1 antibody. Carrier free. Suitable for sELISA and reacts with Rat samples.

View Alternative Names

Ndf, Nrg1, Pro-NRG1

1 Images
Sandwich ELISA - Anti-NRG1 antibody [EPR23075-57] - BSA and Azide free (Capture) (AB281228)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-NRG1 antibody [EPR23075-57] - BSA and Azide free (Capture) (AB281228)

Example of Rat NRG1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23075-57

Isotype

IgG

Carrier free

Yes

Reacts with

Rat

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab281228 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Rat Nrg1. The recommended pair for sandwich ELISA is:
Capture: ab281228, Rat Nrg1 Capture Antibody (unconjugated)
Detector: ab281078, Rat Nrg1 Detector Antibody (unconjugated)
The reference range value is 125 - 8000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuregulin 1 (NRG1) also known as heregulin beta 1 is a member of the neuregulin protein family. It is a signaling protein with a molecular mass that typically ranges around 44 kDa. Researchers have found NRG1 expressed in various tissues including the heart brain and certain epithelial cells. It plays important roles in cell-cell communication by binding to ErbB family receptors particularly ErbB3 and ErbB4 which trigger downstream signaling processes.
Biological function summary

NRG1 is involved in several cellular activities such as cell growth survival and differentiation. It is not part of a complex but acts directly through its interactions with receptor tyrosine kinases in the ErbB receptor family. NRG1 influences cardiac development and neural function by mediating these cellular processes promoting the development and maintenance of neural and cardiac tissues.

Pathways

NRG1 is involved in the PI3K-Akt and MAPK/ERK signaling pathways. These pathways are critical for cellular growth differentiation and survival supporting the functions of NRG1 in various tissues. The interaction with ErbB2 and ErbB3 receptors is especially significant for these pathways as they help regulate the cellular responses initiated by NRG1 signaling.

NRG1 plays an impactful role in the development of certain types of cancer and schizophrenia. Dysregulation or mutations in the NRG1 gene can lead to altered signaling pathways which are connected to the pathogenesis of these conditions. In cancer the interaction with ErbB2 has been under investigation as it may promote tumorigenesis. In the context of schizophrenia NRG1's effects on neural development and neurotransmission are linked with the disorder's symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Binds to ERBB4 and ERBB3. Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV : ITGB3 or ITGA6 : ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling (By similarity). Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1, and ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons (PubMed : 17250808).
See full target information Nrg1
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Product promise

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