Anti-NRG1 type III antibody

• IHC-Fr

Collaborator

Immunohistochemistry (Frozen sections) - Anti-NRG1 type III antibody (AB23248)

ab23248 detecting NRG1 Type III in mouse L4/5 dorsal root ganglion (DRG) tissue (perfused with 4% paraformaldehyde and cryoprotected overnight, cut on a cryostat at 15μm). Tissue was incubated in blocking serum (normal goat serum) for 1hr, washed 3XPBS before primary antibody incubation in PBS with triton (0.1%) overnight (ab23248 was used at 1/800 = 0.34μg/ml). Secondary antibody used was goat anti-rabbit Alexa 546 (1/1000). The image shown is taken with a X20 objective and shows mostly nuclear NRG1 Type III staining with some cytomplasmic staining (nuclear counterstain with DAPI confirmed this - not shown).

This image is courtesy of David Bennett, Kings College London, United Kingdom

• WB

Project

Western blot - Anti-NRG1 type III antibody (AB23248)

ab23248 picks up an ~50kDa band in WB when tested on rat sciatic nerve, rat brain or mouse brain. This band size concurrs with an entry for NRG1 in both Expasy and NCBI databases (Q3URY7). Carroll et al., (J Neurosci. 1997 Mar 1;17(5) : 1642-59) also detect a 50kDa band in dorsal root ganglion and spinal cord. However, it is surprising that we do not detect more bands; it is likely that ab23248 does not pick up all NRG1 type III isoforms or that there is very restricted expression in the tissues tested here.

All lanes:

Western blot - Anti-NRG1 type III antibody (ab23248) at 1 µg/mL

Lane 1:

Mouse Sciatic Nerve Lysate at 20 µg

Lane 2:

Mouse Brain at 20 µg

Lane 3:

Brain (Rat) Tissue Lysate (<a href='/en-us/products/unavailable/brain-rat-tissue-lysate-ab7942'>ab7942</a>) at 20 µg

Secondary

All lanes:

Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution

Observed band size: 52 kDa

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• WB

Lab

Western blot - Anti-NRG1 type III antibody (AB23248)

All lanes:

Western blot - Anti-NRG1 type III antibody (ab23248) at 1 µg/mL

Lanes 1 and 3:

Mouse Brain Tissue Lysate at 10 µg

Lanes 2 and 4:

Rat Brain Tissue Lysate at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 0.05 µg/mL

Lanes 3 - 4:

Western blot - VHH Single Domain Anti-Rabbit IgG Fc (HRP) (<a href='/en-us/products/secondary-antibodies/vhh-single-domain-rabbit-igg-fc-hrp-ab191866'>ab191866</a>) at 0.05 µg/mL

Predicted band size: 11 kDa

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