Rabbit Polyclonal NRG1 antibody. Suitable for WB, IHC-Fr and reacts with Mouse, Rat samples. Cited in 7 publications.
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- Anatomical record (Hoboken, N.J. : 2007) 304:2506-25202021Effect of Schwann cell transplantation combined with electroacupuncture on axonal regeneration and remyelination in rats with spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChengfang Tan,Cheng Yang,Hui Liu,Chenglin Tang,Siqin HuangPubMed 34319000
- eLife 9:2020Subcellular sorting of neuregulins controls the assembly of excitatory-inhibitory cortical circuits.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDavid Exposito-Alonso,Catarina Osório,Clémence Bernard,Sandra Pascual-García,Isabel Del Pino,Oscar Marín,Beatriz RicoPubMed 33320083
- The European journal of neuroscience 52:3306-33212020Juxtacrine signalling via Notch and ErbB receptors in the switch to fate commitment of bone marrow-derived Schwann cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGraham Ka-Hon Shea,Evelyn Wing-Yin Tai,Katherine Ho-Yan Leung,Alan Kwan-Long Mung,Maximilian Tak-Sui Li,Alex Yat-Ping Tsui,Anthony Kin-Wai Tam,Daisy Kwok-Yan Shum,Ying-Shing ChanPubMed 32460437
- Neuroscience 404:510-5182019Neuregulin-1 Accelerates Functional Motor Recovery by Improving Motoneuron Survival After Brachial Plexus Root Avulsion in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuangxi Chen et. al.PubMed 30731156
- Acta neuropathologica communications 6:1002018Alzheimer's associated amyloid and tau deposition co-localizes with a homeostatic myelin repair pathway in two mouse models of post-stroke mixed dementia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThuy-Vi V Nguyen et. al.PubMed 30249297
- Cell proliferation 49:720-7282016Low-intensity pulsed ultrasound upregulates pro-myelination indicators of Schwann cells enhanced by co-culture with adipose-derived stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Yue et. al.PubMed 27625295
- Histochemistry and cell biology 137:829-392012Myelination in coculture of established neuronal and Schwann cell lines.Applications:ICC/IFReactive species:RatKazunori Sango,Emiko Kawakami,Hiroko Yanagisawa,Shizuka Takaku,Masami Tsukamoto,Kazunori Utsunomiya,Kazuhiko WatabePubMed 22366958
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-Fr | |
|---|---|---|
| Human | Predicted | Predicted |
| Mouse | Tested | Tested |
| Rat | Tested | Expected |
| Chicken | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
Species Rat | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Human | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.34 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Human | Dilution info - | Notes - |
Target data
Function
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4 (PubMed:10867024, PubMed:7902537). Binds to ERBB3 (PubMed:20682778). Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1 (PubMed:20682778). Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons (By similarity).
Alternative names
GGF, HGL, HRGA, NDF, SMDF, NRG1, Pro-NRG1
Recommended products
Rabbit Polyclonal NRG1 antibody. Suitable for WB, IHC-Fr and reacts with Mouse, Rat samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
ab23248 detects ~50-55kDa band in rat sciatic nerve, rat and mouse brain lysate. IHC detection with ab23248 in mouse DRG shows a primarily nuclear localisation of the protein rather than the expected cytoplasmic staining (See Bao and Wang references).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NRG1 type III also known as neuregulin-1 protein is an isoform of the neuregulin-1 protein family. It has a mass of approximately 44 kDa. This protein is expressed mainly in the nervous system including neurons and glial cells and is known to play key roles in nervous system function and development. NRG1 type III facilitates cell signaling by binding to ErbB receptors which are a category of epidermal growth factor receptors.
- Biological function summary
This protein variant plays an important role in myelination which is the process of forming a myelin sheath around the nerves. NRG1 type III interacts with the ErbB2 and ErbB3 receptors forming a complex important for these roles. This interaction encourages Schwann cells to initiate and sustain the myelination of peripheral nerves which is vital for proper nerve function and rapid signal transmission.
- Pathways
NRG1 type III is actively involved in the PI3K/Akt and MAPK pathways. It interacts with proteins like ErbB2 and ErbB3 to propagate cell growth and survival signals. These pathways influence several cellular processes including cell growth proliferation and survival thereby facilitating communication between neurons and supporting their structural integrity.
- Associated diseases and disorders
NRG1 type III is linked to schizophrenia and Charcot-Marie-Tooth disease. Alterations in NRG1 type III and its pathway components may lead to disrupted neural signaling and connectivity contributing to the pathophysiology of schizophrenia. Furthermore abnormal expression levels of NRG1 type III are associated with Charcot-Marie-Tooth disease a disorder affecting peripheral nerves often in association with changes in the ErbB2 and ErbB3 receptor interaction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-NRG1 type III antibody (ab23248)
All lanes: Western blot - Anti-NRG1 type III antibody (ab23248) at 1 µg/mL
Lanes 1 and 3: Mouse Brain Tissue Lysate at 10 µg
Lanes 2 and 4: Rat Brain Tissue Lysate at 10 µg
SecondaryLanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 0.05 µg/mL
Lanes 3 - 4: Western blot - VHH Single Domain Anti-Rabbit IgG Fc (HRP) (VHH Single Domain Anti-Rabbit IgG Fc (HRP) ab191866) at 0.05 µg/mL
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
This image is courtesy of David Bennett, Kings College London, United KingdomImmunohistochemistry (Frozen sections) - Anti-NRG1 type III antibody (ab23248)
ab23248 detecting NRG1 Type III in mouse L4/5 dorsal root ganglion (DRG) tissue (perfused with 4% paraformaldehyde and cryoprotected overnight, cut on a cryostat at 15μm). Tissue was incubated in blocking serum (normal goat serum) for 1hr, washed 3XPBS before primary antibody incubation in PBS with triton (0.1%) overnight (ab23248 was used at 1/800 = 0.34μg/ml). Secondary antibody used was goat anti-rabbit Alexa 546 (1/1000). The image shown is taken with a X20 objective and shows mostly nuclear NRG1 Type III staining with some cytomplasmic staining (nuclear counterstain with DAPI confirmed this - not shown).
Western blot - Anti-NRG1 type III antibody (ab23248)
ab23248 picks up an ~50kDa band in WB when tested on rat sciatic nerve, rat brain or mouse brain. This band size concurrs with an entry for NRG1 in both Expasy and NCBI databases (Q3URY7). Carroll et al., (J Neurosci. 1997 Mar 1;17(5):1642-59) also detect a 50kDa band in dorsal root ganglion and spinal cord. However, it is surprising that we do not detect more bands; it is likely that ab23248 does not pick up all NRG1 type III isoforms or that there is very restricted expression in the tissues tested here.
All lanes: Western blot - Anti-NRG1 type III antibody (ab23248) at 1 µg/mL
Lane 1: Mouse Sciatic Nerve Lysate at 20 µg
Lane 2: Mouse Brain at 20 µg
Lane 3: Brain (Rat) Tissue Lysate (ab7942) at 20 µg
SecondaryAll lanes: Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Observed band size: 52 kDa
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