AB109256

Anti-NRG3 antibody [EPR4148]

Name: Anti-NRG3 antibody [EPR4148] (ab109256) | Abcam
Brand: Abcam Limited
SKU: AB109256
Price: 330 USD
Availability: InStock
Rating: 3 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal NRG3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

Pro-NRG3, NRG3

2 Images

Flow Cytometry (Intracellular) - Anti-NRG3 antibody [EPR4148] (AB109256)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-NRG3 antibody [EPR4148] (AB109256)

Intracellular flow cytometric analysis of permeabilized C6 (Rat glial tumor cell line) cells using ab109256 (red) or a rabbit IgG (negative) (green).

Unknown

Western blot - Anti-NRG3 antibody [EPR4148] (AB109256)

All lanes:

Western blot - Anti-NRG3 antibody [EPR4148] (ab109256) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

SH-SY5Y lysate at 10 µg

Lane 3:

C6 lysate at 10 µg

Lane 4:

Mouse brain lysate at 10 µg

Lane 5:

Rat brain lysate at 10 µg

Predicted band size: 77 kDa

Observed band size: 56 kDa

false

Carrier free

Anti-NRG3 antibody [EPR4148] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4148

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NRG3 also known as Neuregulin-3 is a protein with a molecular mass around 71 kDa. It is a member of the neuregulin family which includes proteins that play roles in cell communication and development. NRG3 is expressed in the central nervous system with notable expression in brain regions like the cerebral cortex and hippocampus. It acts mechanically as a signaling molecule binding to ErbB4 a receptor tyrosine kinase to initiate downstream signaling cascades.

Biological function summary

NRG3 influences neural development and synaptic plasticity contributing to the formation and maturation of neuronal circuits. It is part of the epidermal growth factor (EGF)-like family which is involved in cell-to-cell communication necessary for various aspects of development. This protein interacts with ErbB4 to form a receptor-ligand complex essential for signal transduction. These interactions play important roles in brain development and maintenance of normal neural functions.

Pathways

NRG3 plays a role in pathways like the ErbB signaling and the PI3K/AKT pathways. It engages in interactions majorly with ErbB4 facilitating neuronal signaling processes essential for brain function. In the PI3K/AKT pathway NRG3-ErbB4 interaction supports cell survival and growth linking it with other signaling proteins such as PI3K and AKT. This positions NRG3 as an important player in maintaining cellular responses and neuronal homeostasis.

NRG3 has connections with schizophrenia and autism spectrum disorders. Alterations in NRG3 signaling have been associated with the pathophysiology of schizophrenia where abnormal ErbB4-NRG3 interactions contribute to neural development issues leading to the disease. Changes in the signaling pathway involving NRG3 and ErbB4 also relate to autism spectrum disorders indicating its role in neural developmental disorders. Understanding NRG3's function and interactions could provide insights into therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.

See full target information NRG3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

eLife 12: PubMed37432876

2023

induces glutamatergic signaling following treatment with monoaminergic antidepressants.

Applications

Unspecified application

Species

Unspecified reactive species

Rixing Lin,Aron Kos,Juan Pablo Lopez,Julien Dine,Laura M Fiori,Jennie Yang,Yair Ben-Efraim,Zahia Aouabed,Pascal Ibrahim,Haruka Mitsuhashi,Tak Pan Wong,El Cherif Ibrahim,Catherine Belzung,Pierre Blier,Faranak Farzan,Benicio N Frey,Raymond W Lam,Roumen Milev,Daniel J Muller,Sagar V Parikh,Claudio Soares,Rudolf Uher,Corina Nagy,Naguib Mechawar,Jane A Foster,Sidney H Kennedy,Alon Chen,Gustavo Turecki

Molecular medicine reports 17:4611-4618 PubMed29328454

2018

Resveratrol ameliorates depressive disorder through the NETRIN1-mediated extracellular signal-regulated kinase/cAMP signal transduction pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Wang,Jinhui Wang,Jinghong An,Guoming Yuan,Xiaolei Hao,Yi Zhang

Oncotarget 7:53153-53164 PubMed27449290

2016

KRASG12 mutant induces the release of the WSTF/NRG3 complex, and contributes to an oncogenic paracrine signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Shu-Qing Wang,Yue-Hong Long,Su Chen,Yu-Feng Li,Jing-Hua Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NRG3 antibody [EPR4148]

Flow Cytometry (Intracellular) - Anti-NRG3 antibody [EPR4148] (AB109256)

Western blot - Anti-NRG3 antibody [EPR4148] (AB109256)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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