AB169548

Anti-NRK1 antibody [EPR11190]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal NRK1 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 4 publications.

View Alternative Names

C9orf95, NRK1, NMRK1, Nicotinamide riboside kinase 1, NRK 1, NmR-K 1, Nicotinic acid riboside kinase 1, Ribosylnicotinamide kinase 1, Ribosylnicotinic acid kinase 1, RNK 1

3 Images

Flow Cytometry (Intracellular) - Anti-NRK1 antibody [EPR11190] (AB169548)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-NRK1 antibody [EPR11190] (AB169548)

Intracellular flow cytometric analysis of permeabilized HT-29 cells labeling NRK1 with ab169548 at 1/10 dilution (red) compared a rabbit IgG negative control (green).

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Western blot - Anti-NRK1 antibody [EPR11190] (AB169548)

All lanes:

Western blot - Anti-NRK1 antibody [EPR11190] (ab169548) at 1/10000 dilution

Lane 1:

IM-9 cell lysate at 10 µg

Lane 2:

LoVo cell lysate at 10 µg

Lane 3:

HT-29 cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 23 kDa

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CiteAb

Western blot - Anti-NRK1 antibody [EPR11190] (AB169548)

NRK1 western blot using anti-NRK1 antibody [EPR11190] ab169548. Publication image and figure legend from Wilk, A., Hayat, F., et al., 2020, Sci Rep, PubMed 31959836.

ab169548 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab169548 please see the product overview.

Effect of NAMPT inhibition on intracellular NAD+ content in cancer cell lines with different basal levels of CD73 protein. (A) Intracellular NAD+ levels in cells treated with FK866 (30 nM) and/or nicotinamide (NAM) (100 μM), as compared to untreated controls. (B) Intracellular NAD+ levels in cells treated with FK866 (30 nM) and/or nicotinamide riboside (NR) (100 μM), as compared to untreated controls. (C) Intracellular NAD+ levels in cells treated with FK866 (30 nM) and/or nicotinamide mononucleotide (NMN) (100 μM), as compared to untreated controls. (D)Top panel : Representative immunoblot analysis of NRK1 expression to correlate the expression of NRK1 in four cancer cell lines (see Supplement Fig. 3E for additional immunoblot figures). Bottom panel : Densitometry analysis of 3 independent immunoblots, performed using Image Lab software. Statistical analysis was performed using GraphPad Prism 7 - one or two-way ANOVA followed by post-hoc test with Tukey's correction was used (ns = not significant, *p = 0.0176, **p = 0.0018, ***p = 0.0009 or ****p < 0.0001).

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11190

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NRK1 also known as nicotinamide riboside kinase 1 is an enzyme involved in the NAD+ biosynthesis pathway. It functions by phosphorylating nicotinamide riboside (NR) to generate nicotinamide mononucleotide (NMN) an important precursor for NAD+ synthesis. The molecular mass of NRK1 is approximately 34 kDa. This protein is expressed in various tissues including liver brain and muscle where it contributes to the regulation of cellular NAD+ levels.

Biological function summary

This protein plays an important role in cellular energy metabolism and regulation. NRK1 is not part of a larger complex but interacts directly with its substrate nicotinamide riboside. By catalyzing the conversion of NR to NMN NRK1 supports the maintenance of NAD+ levels inside cells which is essential for numerous metabolic processes including those related to energy production and cellular stress responses. NRK1 activity helps regulate critical cellular functions such as DNA repair and mitochondrial function.

Pathways

Nicotinamide riboside kinase 1 integrates into the NAD+ biosynthesis pathway. This pathway is important for maintaining adequate NAD+ levels necessary for various enzymes like sirtuins and poly(ADP-ribose) polymerases (PARPs) that rely on NAD+ as a co-factor. The NRK1-mediated conversion process is closely related to other key proteins such as NRK2 which shares similar enzymatic activity and roles in NAD+ metabolism. Together these proteins contribute to the cellular mechanisms that control energy metabolism and stress responses.

Nicotinamide riboside kinase 1 has shown relevance to metabolic disorders such as obesity and diabetes. Dysregulation of NAD+ metabolism partly attributed to altered NRK1 activity can influence the onset and progression of these conditions. Additionally the role of NRK1 in supporting healthy NAD+ levels connects it to proteins like SIRT1 which mediates beneficial effects on metabolism and longevity. Understanding NRK1's involvement in these pathways may advance therapeutic strategies for metabolic and age-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the phosphorylation of nicotinamide riboside (NR) and nicotinic acid riboside (NaR) to form nicotinamide mononucleotide (NMN) and nicotinic acid mononucleotide (NaMN). The enzyme also phosphorylates the antitumor drugs tiazofurin and 3-deazaguanosine.

See full target information NMRK1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer discovery 12:1984-2005 PubMed35674408

2022

Stress Granules Determine the Development of Obesity-Associated Pancreatic Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Fonteneau,Alexandra Redding,Hannah Hoag-Lee,Edward S Sim,Stefan Heinrich,Matthias M Gaida,Elda Grabocka

Scientific reports 10:651 PubMed31959836

2020

Extracellular NAD enhances PARP-dependent DNA repair capacity independently of CD73 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Wilk,Faisal Hayat,Richard Cunningham,Jianfeng Li,Silvia Garavaglia,Leila Zamani,Davide M Ferraris,Peter Sykora,Joel Andrews,Jennifer Clark,Amanda Davis,Laurent Chaloin,Menico Rizzi,Marie Migaud,Robert W Sobol

Antioxidants & redox signaling 31:1150-1165 PubMed31456414

2019

Subcellular Characterization of Nicotinamide Adenine Dinucleotide Biosynthesis in Metastatic Melanoma by Using Organelle-Specific Biosensors.

Applications

Unspecified application

Species

Unspecified reactive species

Federica Gaudino,Ilaria Manfredonia,Antonella Managò,Valentina Audrito,Nadia Raffaelli,Tiziana Vaisitti,Silvia Deaglio

Nature 569:570-575 PubMed31019297

2019

NAD metabolic dependency in cancer is shaped by gene amplification and enhancer remodelling.

Applications

Unspecified application

Species

Unspecified reactive species

Sudhir Chowdhry,Ciro Zanca,Utkrisht Rajkumar,Tomoyuki Koga,Yarui Diao,Ramya Raviram,Feng Liu,Kristen Turner,Huijun Yang,Elizabeth Brunk,Junfeng Bi,Frank Furnari,Vineet Bafna,Bing Ren,Paul S Mischel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NRK1 antibody [EPR11190]

Flow Cytometry (Intracellular) - Anti-NRK1 antibody [EPR11190] (AB169548)

Western blot - Anti-NRK1 antibody [EPR11190] (AB169548)

Western blot - Anti-NRK1 antibody [EPR11190] (AB169548)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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