Rabbit Recombinant Monoclonal NRP2 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples.
Images
![Flow Cytometry - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (AB273591), expandable thumbnail](https://content.abcam.com/products/images/nrp2-antibody-epr23808-72-bsa-and-azide-free-ab273591--flow-cytometry-img62968.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (AB273591)")
![Immunocytochemistry/ Immunofluorescence - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (AB273591), expandable thumbnail](https://content.abcam.com/products/images/nrp2-antibody-epr23808-72-bsa-and-azide-free-ab273591--immunocytochemistry-immunofluorescence-img19106.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (AB273591)")
![Immunoprecipitation - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (AB273591), expandable thumbnail](https://content.abcam.com/products/images/nrp2-antibody-epr23808-72-bsa-and-azide-free-ab273591--immunoprecipitation-img8564.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (AB273591)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
High affinity receptor for semaphorins 3C, 3F, VEGF-165 and VEGF-145 isoforms of VEGF, and the PLGF-2 isoform of PGF. (Microbial infection) Acts as a receptor for human cytomegalovirus pentamer-dependent entry in epithelial and endothelial cells.
Alternative names
VEGF165R2, NRP2, Neuropilin-2, Vascular endothelial cell growth factor 165 receptor 2
Recommended products
Rabbit Recombinant Monoclonal NRP2 antibody. Carrier free. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23808-72
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab273591 is the carrier-free version of Anti-NRP2 antibody [EPR23808-72] ab273584.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NRP2 also known as Neuropilin-2 is a receptor protein involved in developmental processes including axon guidance and angiogenesis. The molecular mass of NRP2 is approximately 120 kDa. It is expressed in various tissues such as the nervous system and the vascular system but its expression can also be found in certain immune cells. NRP2 plays an important role in cellular signaling by binding to semaphorins and vascular endothelial growth factors (VEGF).
- Biological function summary
NRP2 influences several processes including axonal guidance and the formation of blood vessels. It acts as a co-receptor by forming complexes with other proteins to mediate cellular responses. This role in signaling helps guide neuron growth and regulates vascular development. The NRP2-associated complexes often modulate responses to external signals contributing to the precise control of network formation in tissues.
- Pathways
NRP2 plays a significant role in the VEGF signaling pathway and the semaphorin signaling pathway. These pathways are essential for cell growth migration and differentiation. NRP2 works closely with VEGF receptors like VEGFR2 to promote angiogenic responses. It also interacts with semaphorin family proteins to guide neuronal development. Together these pathways ensure the proper formation and function of neural and vascular networks.
- Associated diseases and disorders
NRP2 is connected to cancer progression and cardiovascular diseases. Altered expression of NRP2 has been observed in various cancer types where it may contribute to tumor growth and metastasis. In these diseases NRP2's interaction with its partners such as VEGFR2 becomes dysregulated affecting normal cellular processes. Cardiovascular conditions can also arise from issues in NRP2-related signaling where the normal function of blood vessel formation is compromised.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591), expandable thumbnail](https://content.abcam.com/products/images/nrp2-antibody-epr23808-72-bsa-and-azide-free-ab273591--flow-cytometry-img62968.jpg "Flow Cytometry - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)")
Flow Cytometry - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)
Flow cytometric analysis of MCF7 (Human breast adenocarcinoma epithelial cell, Left) / U-87 MG (Human glioblastoma-astrocytoma epithelial cell, Right) cells labelling NRP2 with Anti-NRP2 antibody [EPR23808-72] ab273584 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: MCF7.
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab273584).
![Immunocytochemistry/ Immunofluorescence - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591), expandable thumbnail](https://content.abcam.com/products/images/nrp2-antibody-epr23808-72-bsa-and-azide-free-ab273591--immunocytochemistry-immunofluorescence-img19106.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)")
Immunocytochemistry/ Immunofluorescence - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-87 MG cells labelling NRP2 with Anti-NRP2 antibody [EPR23808-72] ab273584 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in U-87 MG cell line. Negative control: MCF7 (PMID:18818766). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/202 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab273584).
![Immunoprecipitation - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591), expandable thumbnail](https://content.abcam.com/products/images/nrp2-antibody-epr23808-72-bsa-and-azide-free-ab273591--immunoprecipitation-img8564.jpg "Immunoprecipitation - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)")
Immunoprecipitation - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (ab273591)
NRP2 was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate with Anti-NRP2 antibody [EPR23808-72] ab273584 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-NRP2 antibody [EPR23808-72] ab273584 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate 10 ug
Lane 2: Anti-NRP2 antibody [EPR23808-72] ab273584 IP in U-87 MG whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NRP2 antibody [EPR23808-72] ab273584 in U-87 MG whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (abab273584).
All lanes: Immunoprecipitation - Anti-NRP2 antibody [EPR23808-72] (Anti-NRP2 antibody [EPR23808-72] ab273584)
Predicted band size: 105 kDa
Observed band size: 130 kDa
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