Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (AB273591)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-87 MG cells labelling NRP2 with ab273584 at 1/100 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic and membranous staining in U-87 MG cell line. Negative control : MCF7 (PMID : 18818766). ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/202 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273584).

• Flow Cyt

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Flow Cytometry - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (AB273591)

Flow cytometric analysis of MCF7 (Human breast adenocarcinoma epithelial cell, Left) / U-87 MG (Human glioblastoma-astrocytoma epithelial cell, Right) cells labelling NRP2 with ab273584 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Negative control : MCF7.

Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273584).

• IP

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Immunoprecipitation - Anti-NRP2 antibody [EPR23808-72] - BSA and Azide free (AB273591)

NRP2 was immunoprecipitated from 0.35 mg U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate with ab273584 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab273584 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate 10 ug

Lane 2 : ab273584 IP in U-87 MG whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab273584 in U-87 MG whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab273584).

All lanes:

Immunoprecipitation - Anti-NRP2 antibody [EPR23808-72] (<a href='/en-us/products/primary-antibodies/nrp2-antibody-epr23808-72-ab273584'>ab273584</a>)

Predicted band size: 105 kDa

Observed band size: 130 kDa

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