Rabbit Polyclonal NS21 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARMC9 aa 400-700.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ARMC9 aa 400-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z3E5
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Orangutan | Dilution info - | Notes - |
Target data
Function
Involved in ciliogenesis (PubMed:32453716). It is required for appropriate acetylation and polyglutamylation of ciliary microtubules, and regulation of cilium length (PubMed:32453716). Acts as a positive regulator of hedgehog (Hh)signaling (By similarity). May participate in the trafficking and/or retention of GLI2 and GLI3 proteins at the ciliary tip (By similarity).
Alternative names
KIAA1868, ARMC9, LisH domain-containing protein ARMC9, Armadillo repeat-containing protein 9, Melanoma/melanocyte-specific tumor antigen KU-MEL-1, NS21
Recommended products
Rabbit Polyclonal NS21 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ARMC9 aa 400-700.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ARMC9 aa 400-700. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z3E5
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NS21 also known as nuclear receptor subfamily 2 group C member 1 functions as a zinc finger nuclear receptor. It has a molecular mass of about 50 kDa. This protein is expressed widely in diverse tissues including the liver brain and kidney. It acts as a transcription factor to regulate the expression of target genes across various systems.
- Biological function summary
NS21 plays a significant role in gene expression modulation by binding to specific DNA response elements. It is not part of a known multi-protein complex but interacts with coactivators and corepressors to exert its function. Through these interactions NS21 can influence cellular processes such as metabolism differentiation and apoptosis. These activities demonstrate its importance in normal cellular homeostasis and functioning.
- Pathways
NS21 participates in the steroid hormone receptor signaling and retinoic acid signaling pathways. It closely interacts with the retinoid X receptor (RXR) within these pathways to regulate gene expression. Through these pathways NS21 affects cellular processes that are critical for development and metabolism. By interacting with RXR it can influence the transcription of genes involved in several essential biological functions.
- Associated diseases and disorders
NS21 involvement has been observed in metabolic disorders and certain types of cancer. The protein has been connected to liver cancer through its regulation of metabolic pathways and interactions with proteins such as RXR. Additionally alterations in NS21 activity can lead to imbalances in metabolic processes contributing to disorders like diabetes. Changes in NS21 function or expression might link to these disease states suggesting the potential for targeted therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Anti-NS21 antibody (ab235416)
All lanes: Western blot - Anti-NS21 antibody (ab235416) at 1/500 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2: CEM whole cell lysate
Lane 3: A549 (human lung carcinoma cell line) whole cell lysate
SecondaryAll lanes: Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 92 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NS21 antibody (ab235416)
Paraffin-embedded human tonsil tissue stained for NS21 using ab235416 at 1/100 dilution in immunohistochemical analysis.
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