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AB235416

Anti-NS21 antibody

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(1 Publication)

Rabbit Polyclonal NS21 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ARMC9 aa 400-700.

View Alternative Names

KIAA1868, ARMC9, LisH domain-containing protein ARMC9, Armadillo repeat-containing protein 9, Melanoma/melanocyte-specific tumor antigen KU-MEL-1, NS21

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NS21 antibody (AB235416)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NS21 antibody (AB235416)

Paraffin-embedded human tonsil tissue stained for NS21 using ab235416 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-NS21 antibody (AB235416)
  • WB

Supplier Data

Western blot - Anti-NS21 antibody (AB235416)

All lanes:

Western blot - Anti-NS21 antibody (ab235416) at 1/500 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

CEM whole cell lysate

Lane 3:

A549 (human lung carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 92 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ARMC9 aa 400-700. The exact immunogen used to generate this antibody is proprietary information.

Q7Z3E5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NS21 also known as nuclear receptor subfamily 2 group C member 1 functions as a zinc finger nuclear receptor. It has a molecular mass of about 50 kDa. This protein is expressed widely in diverse tissues including the liver brain and kidney. It acts as a transcription factor to regulate the expression of target genes across various systems.
Biological function summary

NS21 plays a significant role in gene expression modulation by binding to specific DNA response elements. It is not part of a known multi-protein complex but interacts with coactivators and corepressors to exert its function. Through these interactions NS21 can influence cellular processes such as metabolism differentiation and apoptosis. These activities demonstrate its importance in normal cellular homeostasis and functioning.

Pathways

NS21 participates in the steroid hormone receptor signaling and retinoic acid signaling pathways. It closely interacts with the retinoid X receptor (RXR) within these pathways to regulate gene expression. Through these pathways NS21 affects cellular processes that are critical for development and metabolism. By interacting with RXR it can influence the transcription of genes involved in several essential biological functions.

NS21 involvement has been observed in metabolic disorders and certain types of cancer. The protein has been connected to liver cancer through its regulation of metabolic pathways and interactions with proteins such as RXR. Additionally alterations in NS21 activity can lead to imbalances in metabolic processes contributing to disorders like diabetes. Changes in NS21 function or expression might link to these disease states suggesting the potential for targeted therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in ciliogenesis (PubMed : 32453716). It is required for appropriate acetylation and polyglutamylation of ciliary microtubules, and regulation of cilium length (PubMed : 32453716). Acts as a positive regulator of hedgehog (Hh)signaling (By similarity). May participate in the trafficking and/or retention of GLI2 and GLI3 proteins at the ciliary tip (By similarity).
See full target information ARMC9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106468 PubMed37091236

2023

Latent CSN-CRL complexes are crucial for curcumin-induced apoptosis and recruited during adipogenesis to lipid droplets via small GTPase RAB18.

Applications

Unspecified application

Species

Unspecified reactive species

Dawadschargal Dubiel,Jing Wang,Roland Hartig,Supattra Chaithongyot,Wolfgang Dubiel,Michael Naumann
View all publications
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