AB199730

Anti-NSDHL antibody [EPR14489(2)]

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal NSDHL antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

H105E3, NSDHL, Protein H105e3

5 Images

Supplier Data

Immunoprecipitation - Anti-NSDHL antibody [EPR14489(2)] (AB199730)

NSDHL was immunoprecipitated from 1mg of Human fetal brain whole cell lysate with ab199730 at 1/50 dilution.

Lane 1 : Human fetal brain whole cell lysate 10ug (Input).

Lane 2 : ab199730 IP in Human fetal brain whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199730 in Human fetal brain whole cell lysate.

Western blot was performed from the immunoprecipitate using ab199730 at 1/1000 dilution.

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 5 seconds.

All lanes:

Immunoprecipitation - Anti-NSDHL antibody [EPR14489(2)] (ab199730)

Predicted band size: 42 kDa

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Supplier Data

Immunoprecipitation - Anti-NSDHL antibody [EPR14489(2)] (AB199730)

NSDHL was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab199730 at 1/50 dilution.

Lane 1 : HeLa whole cell lysate 10ug (Input).

Lane 2 : ab199730 IP in HeLa whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199730 in HeLa whole cell lysate.

Western blot was performed from the immunoprecipitate using ab199730 at 1/1000 dilution (Panel A) or ab190353 at 1/1000 dilution (Panel B).

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds (Panel A and B).

ab199730 was used to immunoprecipitate NSDHL. These precipitates were resolved with ab190353.

All lanes:

Immunoprecipitation - Anti-NSDHL antibody [EPR14489(2)] (ab199730)

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-NSDHL antibody [EPR14489(2)] (AB199730)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NSDHL antibody [EPR14489(2)] (ab199730) at 1/1000 dilution

Lane 1:

A431 (Human epidermoid carcinoma) whole cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 3:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

Lane 4:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 5s

Lab

Western blot - Anti-NSDHL antibody [EPR14489(2)] (AB199730)

Lanes 1-4 : Merged signal (red and green). Green - ab199730 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.

ab199730 Anti-NSDHL antibody [EPR14489(2)] was shown to specifically react with NSDHL in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266682 (knockout cell lysate ab258082) was used. Wild-type and NSDHL knockout samples were subjected to SDS-PAGE. ab199730 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-NSDHL antibody [EPR14489(2)] (ab199730) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

NSDHL knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human NSDHL knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-nsdhl-knockout-hek-293t-cell-line-ab266682'>ab266682</a>)

Lane 3:

A431 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 38 kDa

false

Supplier Data

Western blot - Anti-NSDHL antibody [EPR14489(2)] (AB199730)

Blocking buffer : 5% NFDM/TBST.

Dilution buffer : 5% NFDM /TBST or 1%BSA /TBST.

All lanes:

Western blot - Anti-NSDHL antibody [EPR14489(2)] (ab199730) at 1/1000 dilution

Lane 1:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 3:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

Exposure time: 10s

Carrier free

Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14489(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSDHL also known as NAD(P)H steroid dehydrogenase-like is an enzyme linked to the cholesterol biosynthesis pathway. It has a molecular weight of approximately 41 kDa. This enzyme localizes to the endoplasmic reticulum membrane and the peroxisomes. NSDHL shows expression in various tissues with higher levels in the liver adrenal glands and the brain. This distribution highlights its essential role in metabolic processes across different tissue types.

Biological function summary

NSDHL facilitates the removal of one hydrogen from NAD(P)H and adds it to the steroid precursor lathosterol an important intermediate in cholesterol biosynthesis. Although not part of a larger complex NSDHL performs significant enzymatic steps necessary for converting sterol intermediates. Its activity directly influences sterol composition vital for cell membrane integrity signaling and hormone synthesis.

Pathways

The enzyme plays a critical role in the cholesterol biosynthesis pathway specifically affecting the conversion of lanosterol into cholesterol. NSDHL operates in tandem with other enzymes like DHCR7 influencing the downstream process of sterol maturation. The precise coordination of these enzymes ensures effective cholesterol production essential for maintaining cellular cholesterol levels and systemic lipid homeostasis.

Malfunctions in NSDHL can lead to disorders like CHILD syndrome (Congenital Hemidysplasia with Ichthyosiform erythroderma and Limb Defects) and CK syndrome (Conradi-Hünermann-Happle syndrome). These conditions link to cholesterol biosynthesis disruption impacting skin and skeletal development. NSDHL interacts with the EBP protein in these pathways suggesting a relationship between enzyme activity and phenotypic expression in these disorders. Understanding NSDHL's function and regulation is key to recognizing its involvement in metabolic syndromes and potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the NAD(P)(+)-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis (By similarity). Also plays a role in the regulation of the endocytic trafficking of EGFR (By similarity).

See full target information NSDHL

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 11:e829 PubMed37249295

2023

Myo-inositol oxygenase (MIOX) accelerated inflammation in the model of infection-induced cardiac dysfunction by NLRP3 inflammasome.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjun Zhou,Congyi Yu,Yiwen Long

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NSDHL antibody [EPR14489(2)]

Immunoprecipitation - Anti-NSDHL antibody [EPR14489(2)] (AB199730)

Immunoprecipitation - Anti-NSDHL antibody [EPR14489(2)] (AB199730)

Western blot - Anti-NSDHL antibody [EPR14489(2)] (AB199730)

Western blot - Anti-NSDHL antibody [EPR14489(2)] (AB199730)

Western blot - Anti-NSDHL antibody [EPR14489(2)] (AB199730)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise