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Rabbit Recombinant Monoclonal NSDHL antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.

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Images

Western blot - Anti-NSDHL antibody [EPR14490] (AB190353), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (AB190353), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (AB190353), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NSDHL antibody [EPR14490] (AB190353), expandable thumbnail
  • Western blot - Anti-NSDHL antibody [EPR14490] (AB190353), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/10000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/50

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50 - 1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the NAD(P)(+)-dependent oxidative decarboxylation of the C4 methyl groups of 4-alpha-carboxysterols in post-squalene cholesterol biosynthesis (By similarity). Also plays a role in the regulation of the endocytic trafficking of EGFR (By similarity).

Alternative names

H105E3, NSDHL, Protein H105e3

Recommended products

Rabbit Recombinant Monoclonal NSDHL antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR14490

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

NSDHL also known as NAD(P)H steroid dehydrogenase-like is an enzyme linked to the cholesterol biosynthesis pathway. It has a molecular weight of approximately 41 kDa. This enzyme localizes to the endoplasmic reticulum membrane and the peroxisomes. NSDHL shows expression in various tissues with higher levels in the liver adrenal glands and the brain. This distribution highlights its essential role in metabolic processes across different tissue types.

Biological function summary

NSDHL facilitates the removal of one hydrogen from NAD(P)H and adds it to the steroid precursor lathosterol an important intermediate in cholesterol biosynthesis. Although not part of a larger complex NSDHL performs significant enzymatic steps necessary for converting sterol intermediates. Its activity directly influences sterol composition vital for cell membrane integrity signaling and hormone synthesis.

Pathways

The enzyme plays a critical role in the cholesterol biosynthesis pathway specifically affecting the conversion of lanosterol into cholesterol. NSDHL operates in tandem with other enzymes like DHCR7 influencing the downstream process of sterol maturation. The precise coordination of these enzymes ensures effective cholesterol production essential for maintaining cellular cholesterol levels and systemic lipid homeostasis.

Associated diseases and disorders

Malfunctions in NSDHL can lead to disorders like CHILD syndrome (Congenital Hemidysplasia with Ichthyosiform erythroderma and Limb Defects) and CK syndrome (Conradi-Hünermann-Happle syndrome). These conditions link to cholesterol biosynthesis disruption impacting skin and skeletal development. NSDHL interacts with the EBP protein in these pathways suggesting a relationship between enzyme activity and phenotypic expression in these disorders. Understanding NSDHL's function and regulation is key to recognizing its involvement in metabolic syndromes and potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-NSDHL antibody [EPR14490] (ab190353), expandable thumbnail

    Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)

    Lanes 1-4: Merged signal (red and green). Green - ab190353 observed at 38 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.

    ab190353 Anti-NSDHL antibody [EPR14490] was shown to specifically react with NSDHL in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human NSDHL knockout HEK-293T cell line ab266682 (knockout cell lysate Human NSDHL knockout HEK-293T cell lysate ab258082) was used. Wild-type and NSDHL knockout samples were subjected to SDS-PAGE. ab190353 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-NSDHL antibody [EPR14490] (ab190353) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: NSDHL knockout HEK293T cell lysate at 20 µg

    Lane 3: A431 cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 42 kDa

    Observed band size: 38 kDa

    This data was developed using ab190353, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab190353 observed at 38 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.

    ab190353 Anti-NSDHL antibody [EPR14490] was shown to specifically react with NSDHL in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human NSDHL knockout HEK-293T cell line ab266682 (knockout cell lysate Human NSDHL knockout HEK-293T cell lysate ab258082) was used. Wild-type and NSDHL knockout samples were subjected to SDS-PAGE. ab190353 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)

    Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of endometrium tissue labeling NSDHL with ab190353 at 1/100 dilution. Secondary antibody HRP Polymer for Rabbit IgG prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSDHL antibody [EPR14490] (ab190353)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling NSDHL with ab190353 at 1/100 dilution. Secondary antibody HRP Polymer for Rabbit IgG prediluted. Counter stain Hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-NSDHL antibody [EPR14490] (ab190353), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NSDHL antibody [EPR14490] (ab190353)

    Immunofluorescence analysis of U87-MG cells, labeling NSDHL (red) with ab190353 at 1/50 dilution. Alexa Fluor®555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).

  • Western blot - Anti-NSDHL antibody [EPR14490] (ab190353), expandable thumbnail

    Western blot - Anti-NSDHL antibody [EPR14490] (ab190353)

    All lanes: Western blot - Anti-NSDHL antibody [EPR14490] (ab190353) at 1/10000 dilution

    Lane 1: A431 cell lysate at 20 µg

    Lane 2: HeLa cell lysate at 20 µg

    Lane 3: Human fetal brain tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 38 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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