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AB15486

Anti-NSE antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal NSE antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody (AB15486)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody (AB15486)

ab15486 staining NSE in human pancreas by Immunohistochemistry (FFPE-sections).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/300", "IHCP-species-notes": "<p>1/300 for 10 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0 for 10 minutes, followed by cooling at RT for 20 minutes.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuron-specific enolase (NSE) also known simply as enolase-2 is a glycolytic enzyme with a molecular weight of approximately 47 kDa. This protein mainly facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. NSE is expressed significantly in neural tissues and neuroendocrine cells. Researchers often utilize it in various assays including NSE IHC and NSE ELISA to assess its expression levels in different tissue types.
Biological function summary

NSE plays a major role in cellular metabolism particularly in neurons where it aids energy production through glycolysis. It forms part of a dimeric complex bonding typically with itself or other enolase isoforms for proper function. As an important enzyme in the metabolic pathway NSE helps ensure neurons and associated cells maintain energy homeostasis which is fundamental for sustenance and normal cellular functions.

Pathways

NSE is an integral component of glycolysis a metabolic pathway pivotal in cellular energy production. The process collaborates closely with other glycolytic enzymes like pyruvate kinase and hexokinase to facilitate efficient energy release from glucose. Through these interactions NSE guarantees the smooth continuation of glycolysis highlighting its importance within cellular metabolism frameworks.

NSE serves as a marker for neuroblastoma and small cell lung cancer. Elevated NSE levels suggest potential malignancies due to its release into the bloodstream from damaged neural and neuroendocrine cells. Moreover it often appears alongside proteins like neuron-specific markers such as synaptophysin in diagnostic assays. Understanding its involvement in these conditions helps clinicians diagnose and monitor the progression of certain cancers effectively.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Enolase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in glycolysis and the reverse reaction in gluconeogenesis (By similarity). Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).
See full target information ENO2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biochemistry and biophysics reports 44:102217 PubMed40917722

2025

β-Hydroxybutyrate promotes chemoresistance and proliferation in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Milad Mashinchian,Somayeh Vandghanooni,Amir Reza Karamibonari,Morteza Eskandani

RSC advances 11:12423-12433 PubMed35423725

2021

Synthesis and evaluation of 3'-[F]fluorothymidine-5'-squaryl as a bioisostere of 3'-[F]fluorothymidine-5'-monophosphate.

Applications

Unspecified application

Species

Unspecified reactive species

D Brickute,A Beckley,L Allott,M Braga,C Barnes,K J Thorley,E O Aboagye
View all publications
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Product promise

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