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Mouse Monoclonal NSE antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ENO2 aa 400 to C-terminus.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (AB218388), expandable thumbnail
  • Western blot - Anti-NSE antibody [ENO2/1375] - Neuronal Marker (AB218388), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (AB218388), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] - Neuronal Marker (AB218388), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (AB218388), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Synthetic Peptide within Human ENO2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09104

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
0.20000-0.40000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
0.20000-0.40000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
0.20000-0.40000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Target data

Function

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

Alternative names

Recommended products

Mouse Monoclonal NSE antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ENO2 aa 400 to C-terminus.

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Synthetic Peptide within Human ENO2 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P09104
Clone number
ENO2/1375
Purification technique
Affinity purification Protein A/G
Concentration
Loading...
Purification notes

ab218388 is purified from Bioreactor Concentrate by Protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Neuron-specific enolase (NSE) also known simply as enolase-2 is a glycolytic enzyme with a molecular weight of approximately 47 kDa. This protein mainly facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. NSE is expressed significantly in neural tissues and neuroendocrine cells. Researchers often utilize it in various assays including NSE IHC and NSE ELISA to assess its expression levels in different tissue types.

Biological function summary

NSE plays a major role in cellular metabolism particularly in neurons where it aids energy production through glycolysis. It forms part of a dimeric complex bonding typically with itself or other enolase isoforms for proper function. As an important enzyme in the metabolic pathway NSE helps ensure neurons and associated cells maintain energy homeostasis which is fundamental for sustenance and normal cellular functions.

Pathways

NSE is an integral component of glycolysis a metabolic pathway pivotal in cellular energy production. The process collaborates closely with other glycolytic enzymes like pyruvate kinase and hexokinase to facilitate efficient energy release from glucose. Through these interactions NSE guarantees the smooth continuation of glycolysis highlighting its importance within cellular metabolism frameworks.

Associated diseases and disorders

NSE serves as a marker for neuroblastoma and small cell lung cancer. Elevated NSE levels suggest potential malignancies due to its release into the bloodstream from damaged neural and neuroendocrine cells. Moreover it often appears alongside proteins like neuron-specific markers such as synaptophysin in diagnostic assays. Understanding its involvement in these conditions helps clinicians diagnose and monitor the progression of certain cancers effectively.

Product promise

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5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pheochromocytoma tissue labeling NSE with ab218388 at 0.2 μg/mL.

  • Western blot - Anti-NSE antibody [ENO2/1375] - Neuronal Marker (ab218388), expandable thumbnail

    Western blot - Anti-NSE antibody [ENO2/1375] - Neuronal Marker (ab218388)

    NSE Western blot staining using mouse Anti-NSE antibody

    All lanes: Western blot - Anti-NSE antibody [ENO2/1375] - Neuronal Marker (ab218388) at 1 µg/mL

    Lane 1: Y79 cell lysates

    Lane 2: HeLa cell lysates

    Lane 3: HepG2 cell lysates

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cerebellum tissue labeling NSE with ab218388 at 0.2 μg/mL.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] - Neuronal Marker (ab218388), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] - Neuronal Marker (ab218388)

    NSE Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using mouse Anti-NSE antibody

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse pancreas tissue labeling NSE with ab218388 at 0.2 μg/mL.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody [ENO2/1375] (ab218388)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat heart tissue labeling NSE with ab218388 at 0.2 μg/mL.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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