Anti-ENO1 + ENO2 + ENO3 antibody [EPR12483] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR12483] - BSA and Azide free (AB250275)

Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling Tcl1 with purified ab225718 at 1/50 (2.6 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor© 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti nse antibody epr12483 immunocytochemistry neuro2a mouse)

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR12483] - BSA and Azide free (AB250275)

Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD23 with purified ab135386 at 1/25 (7.48 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor© 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti nse antibody epr12483 immunocytochemistry hela human)

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody [EPR12483] - BSA and Azide free (AB250275)

Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling alpha smooth muscle Actin with purified ab150301 at 1/100(1.65 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor© 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti nse antibody epr12483 immunocytochemistry pc12 rat)

• WB

Lab

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR12483] - BSA and Azide free (AB250275)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180943).

Western blot : Anti-ENO1 + ENO2 + ENO3 antibody [EPR12483] ab180943 staining at 1/1000 dilution, shown in green; Mouse anti-6x HisTag (ab18184) loading control staining at 1/20,000 dilution, shown in magenta.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution. For DDDDK-tag detection, HRP Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [M2] (ab49763) was used at 1/1000 dilution and developed using the ECL technique.

Exposure time for DDDDK tag : 0.1s.

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody [EPR12483] (<a href='/en-us/products/primary-antibodies/nse-antibody-epr12483-neuronal-marker-ab180943'>ab180943</a>) at 1/1000 dilution

Lane 1:

ENO1 Recombinant Protein (His Tag) at 0.2 µg

Lane 2:

ENO2 Recombinant Protein (His Tag) at 0.2 µg

Lane 3:

ENO3 Recombinant Protein (His Tag) at 0.2 µg

Lane 4:

ENO1 Recombinant Protein (DDDDK Tag) at 0.2 µg

Lane 5:

ENO2 Recombinant Protein (DDDDK Tag) at 0.2 µg

Lane 6:

ENO3 Recombinant Protein (DDDDK Tag) at 0.2 µg

Secondary

Lanes 1 - 6:

Goat anti-Mouse 680RD at 1/20000 dilution

Lanes 1 - 6:

Goat anti-Rabbit 800CW at 1/20000 dilution

Observed band size: 50 kDa

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