Anti-NSE antibody [EPR12483] - BSA and Azide free

• WB

Lab

Western blot - Anti-NSE antibody [EPR12483] - BSA and Azide free (AB250275)

This data was developed using ab180943, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Anti-DDDDK tag antibody [6F7] - Loading Control (ab205606) staining at 1/1000 dilution.

This antibody has weak cross-reactivity with ENO3.

All lanes:

Western blot - Anti-NSE antibody [EPR12483] - Neuronal Marker (<a href='/en-us/products/primary-antibodies/nse-antibody-epr12483-neuronal-marker-ab180943'>ab180943</a>) at 1/10000 dilution

Lane 1:

Recombinant human ENO1 protein with C-DDDDK tag at 10 ng

Lane 2:

Recombinant human ENO2 (NSE) protein with C-DDDDK tag at 10 ng

Lane 3:

Recombinant human ENO3 protein with C-DDDDK tag at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa

false

Exposure time: 180s

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR12483] - BSA and Azide free (AB250275)

Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (mouse embryonic fibroblast) cells labeling alpha smooth muscle Actin with purified ab150301 at 1/100(1.65 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor© 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti nse antibody epr12483 immunocytochemistry pc12 rat)

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR12483] - BSA and Azide free (AB250275)

Immunocytochemistry/ Immunofluorescence analysis of Ramos (human Burkitt's lymphoma B lymphocyte) cells labeling Tcl1 with purified ab225718 at 1/50 (2.6 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor© 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti nse antibody epr12483 immunocytochemistry neuro2a mouse)

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NSE antibody [EPR12483] - BSA and Azide free (AB250275)

Immunocytochemistry/ Immunofluorescence analysis of Raji (human Burkitt's lymphoma B lymphocyte) cells labeling CD23 with purified ab135386 at 1/25 (7.48 μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor© 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti nse antibody epr12483 immunocytochemistry hela human)