AB53025

Anti-ENO1 + ENO2 + ENO3 antibody

Name: Anti-NSE antibody - Neuronal Marker (ab53025) | Abcam
Brand: Abcam Limited
SKU: AB53025
Price: 400 USD
Availability: InStock
Rating: 4 (11 reviews)

Lab Essentials
What is this?

(11 Reviews)

(69 Publications)

Rabbit Polyclonal NSE antibody. Neuron marker. Suitable for WB, Flow Cyt, ELISA, IHC-FoFr, IHC-P, ICC/IF and reacts with Recombinant fragment - Human, Human, Mouse, Rat, Pig samples. Cited in 69 publications. Immunogen corresponding to Synthetic Peptide within Human ENO2.

View Alternative Names

Gamma-enolase, 2-phospho-D-glycerate hydro-lyase, Enolase 2, Neural enolase, Neuron-specific enolase, NSE, ENO2

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody (AB53025)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody (AB53025)

Immunohistochemical analysis of paraffin-embedded human brain tissue using ab53025 at 1/50-1/100 dilution. Left : without immunizing peptide; Right : with immunizing peptide

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody (AB53025)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody (AB53025)

ICC/IF image of ab53025 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53025, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody (AB53025)

ab53025 staining NSE in mouse brain tissue section by Immunohistochemistry (PFA-perfussion fixed frozen tissue sections). Tissues were collected from mice in each group at 10 weeks. Mice were euthanized, the brains were exposed and removed from the body. Then the brain was cut, on ice and fixed in 4% polyformaldehyde or glutaraldehyde solution for histological examination. The primary antibody was used at 1/75 dilution in PBS and incubated with sample for 2 hours at 37°C. A HRP-conjugated secondary antibody was used at for 1hr at 37°C in a humidity box.

Image from Yu Yang. et. al. Mol. Cell. Proteomics, Mar 2009 (Fig 6).

Lab

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody (AB53025)

Western blot : Anti-ENO1 + ENO2 + ENO3 antibody ab53025 staining at 1/1000 dilution, shown in green; Mouse anti-6x HisTag (ab18184) loading control staining at 1/20,000 dilution, shown in magenta.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody (ab53025) at 1/1000 dilution

Lane 1:

ENO1 Recombinant Protein (His Tag) at 0.2 µg

Lane 2:

ENO2 Recombinant Protein (His Tag) at 0.2 µg

Lane 3:

ENO3 Recombinant Protein (His Tag) at 0.2 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

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Unknown

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody (AB53025)

All lanes:

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody (ab53025) at 1/500 dilution

Lane 1:

Extracts from HepG2 cells with no immunizing peptide

Lane 2:

Extracts from HepG2 cells with immunizing peptide

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Pig, Mouse, Rat

Applications

ELISA, IHC-P, WB, ICC/IF, IHC-FoFr, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human ENO2. The exact immunogen used to generate this antibody is proprietary information.

P09104

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCFoFr" : {"fullname" : "Immunohistochemistry (PFA perfusion fixed frozen sections)", "shortname":"IHC-FoFr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-polyclonal-isotype-control-chip-grade-ab171870'>ab171870</a> - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCFoFr-species-checked": "guaranteed", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCFoFr-species-checked": "notRecommended", "IHCFoFr-species-dilution-info": "", "IHCFoFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage buffer

pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Neuron-specific enolase (NSE) also known simply as enolase-2 is a glycolytic enzyme with a molecular weight of approximately 47 kDa. This protein mainly facilitates the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. NSE is expressed significantly in neural tissues and neuroendocrine cells. Researchers often utilize it in various assays including NSE IHC and NSE ELISA to assess its expression levels in different tissue types.

Biological function summary

NSE plays a major role in cellular metabolism particularly in neurons where it aids energy production through glycolysis. It forms part of a dimeric complex bonding typically with itself or other enolase isoforms for proper function. As an important enzyme in the metabolic pathway NSE helps ensure neurons and associated cells maintain energy homeostasis which is fundamental for sustenance and normal cellular functions.

Pathways

NSE is an integral component of glycolysis a metabolic pathway pivotal in cellular energy production. The process collaborates closely with other glycolytic enzymes like pyruvate kinase and hexokinase to facilitate efficient energy release from glucose. Through these interactions NSE guarantees the smooth continuation of glycolysis highlighting its importance within cellular metabolism frameworks.

NSE serves as a marker for neuroblastoma and small cell lung cancer. Elevated NSE levels suggest potential malignancies due to its release into the bloodstream from damaged neural and neuroendocrine cells. Moreover it often appears alongside proteins like neuron-specific markers such as synaptophysin in diagnostic assays. Understanding its involvement in these conditions helps clinicians diagnose and monitor the progression of certain cancers effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

See full target information ENO2

Additional targets

ENO3,ENO1

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Publications (69)

Recent publications for all applications. Explore the full list and refine your search

European journal of medical research 29:498 PubMed39415292

2024

The deubiquitinase OTUD3 stabilizes IRP2 expression to reduce hippocampal neuron ferroptosis via the p53/PTGS2 pathway to ameliorate cerebral ischemia-reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Hou,Yujie Hu,Tian Yun,Hongxin Li,Guoshuai Yang,Dan Yu

Translational pediatrics 13:1312-1326 PubMed39263295

2024

Changes in short-chain fatty acids affect brain development in mice with early life antibiotic-induced dysbacteriosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qixing Zhang,Han Li,Shuang Yin,Feng Xiao,Chen Gong,Jie Zhou,Kangkang Liu,Yan Cheng

Frontiers in bioengineering and biotechnology 12:1415527 PubMed38933542

2024

Genetically engineered electrospinning contributes to spinal cord injury repair by regulating the immune microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Sun,Jie Wu,Liang Zhou,Wei Wang,Haibo Wang,Shaosong Sun,Yichang Xu,Lichen Zhang,Xinzhao Jiang,Guoqing Zhu,Kun Xi,Yong Gu,Liang Chen

Annals of translational medicine 11:197 PubMed37007562

2023

Inhibition of NF-κB ameliorates aberrant retinal glia activation and inflammatory responses in streptozotocin-induced diabetic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Ding,Zhongcui Sun,Yue Guo,Wenyi Tang,Qinmeng Shu,Gezhi Xu

Journal of neuroinflammation 20:31 PubMed36765376

2023

Morinda officinalis oligosaccharides mitigate depression-like behaviors in hypertension rats by regulating Mfn2-mediated mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Lixuan Yang,Yutian Ao,Yannan Li,Baoan Dai,Jingchun Li,Wenzhe Duan,Wei Gao,Zhonghui Zhao,Zhenyun Han,Rongjuan Guo

Journal of biochemical and molecular toxicology 37:e23236 PubMed36239013

2022

Protein kinase C beta relieves autism-like behavior in EN2 knockout mice via upregulation of the FTO/PGC-1α/UCP1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyu Song,Qibo Hu,Xiaoheng Xu,Wei Pan

Discover. Oncology 13:103 PubMed36227363

2022

Combination bromo- and extraterminal domain and poly (ADP-ribose) polymerase inhibition synergistically enhances DNA damage and inhibits neuroblastoma tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jillian C Jacobson,Jingbo Qiao,Rachael A Clark,Dai H Chung

Cancer science 112:2884-2894 PubMed33934428

2021

Specific activation of glycolytic enzyme enolase 2 in BRAF V600E-mutated colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ryohei Yukimoto,Naohiro Nishida,Tsuyoshi Hata,Shiki Fujino,Takayuki Ogino,Norikatsu Miyoshi,Hidekazu Takahashi,Mamoru Uemura,Taroh Satoh,Yamamoto Hirofumi,Tsunekazu Mizushima,Yuichiro Doki,Hidetoshi Eguchi

Frontiers in medicine 8:643402 PubMed33829024

2021

Evaluation of the Effectiveness of a Chronic Ocular Hypertension Mouse Model Induced by Intracameral Injection of Cross-Linking Hydrogel.

Applications

Unspecified application

Species

Unspecified reactive species

Junjue Chen,Jun Sun,Huan Yu,Ping Huang,Yisheng Zhong

PloS one 16:e0248777 PubMed33735260

2021

Sulforaphane (SFA) protects neuronal cells from oxygen & glucose deprivation (OGD).

Applications

Unspecified application

Species

Unspecified reactive species

Zeenat Ladak,Elizabeth Garcia,Jenny Yoon,Takaaki Landry,Edward A Armstrong,Jerome Y Yager,Sujata Persad

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ENO1 + ENO2 + ENO3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody (AB53025)

Immunocytochemistry/ Immunofluorescence - Anti-ENO1 + ENO2 + ENO3 antibody (AB53025)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 + ENO2 + ENO3 antibody (AB53025)

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody (AB53025)

Western blot - Anti-ENO1 + ENO2 + ENO3 antibody (AB53025)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (69)

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