AB95963

Anti-NSFL1C antibody

Name: Anti-NSFL1C antibody (ab95963) | Abcam
Brand: Abcam Limited
SKU: AB95963
Rating: 4 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal NSFL1C antibody. Suitable for WB, IHC-P, IP and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NSFL1C aa 1-300.

View Alternative Names

UBXN2C, NSFL1C, NSFL1 cofactor p47, UBX domain-containing protein 2C, p97 cofactor p47

4 Images

Supplier Data

Immunoprecipitation - Anti-NSFL1C antibody (AB95963)

Immunoprecipitation of NSFL1C protein from A431 whole cell extracts using 5 µg of NSFL1C antibody. Western blot analysis was performed using NSFL1C antibody.

All lanes:

Immunoprecipitation - Anti-NSFL1C antibody (ab95963) at 5 µg

Lane 1:

A431 whole cell extracts

Lane 2:

Control with IgG

Lane 3:

Immunoprecipitated A431 whole cell extracts using ab95963

Secondary

All lanes:

Anti-Rabbit IgG

Predicted band size: 41 kDa

false

Supplier Data

Western blot - Anti-NSFL1C antibody (AB95963)

Samples were separated by 10% SDS PAGE.

All lanes:

Western blot - Anti-NSFL1C antibody (ab95963) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 41 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSFL1C antibody (AB95963)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSFL1C antibody (AB95963)

ab95963, at 1/100, staining paraffin-embedded differential N/T pair Lung SCC cancer by Immunohistochemistry.

Unknown

Western blot - Anti-NSFL1C antibody (AB95963)

All lanes:

Western blot - Anti-NSFL1C antibody (ab95963) at 1/1000 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

A431 whole cell lysate at 30 µg

Lane 3:

H1299 whole cell lysate at 30 µg

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human NSFL1C aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9UNZ2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSFL1C also known as p97 cofactor p47 is a protein involved in cellular functions related to NSF ATPases. The protein has a mass of approximately 47 kDa. NSFL1C is expressed in various tissues throughout the body where it plays an active role in modulating interactions between cellular structures. This protein mainly functions by regulating membrane fusion events and protein degradation working closely with other components to maintain cellular integrity.

Biological function summary

NSFL1C acts as an important modulator in cellular homeostasis often participating in complexes with other proteins like p97/VCP a well-known AAA ATPase. This partnership allows NSFL1C to influence diverse cellular activities including endoplasmic-reticulum-associated protein degradation (ERAD) ensuring the removal of misfolded proteins. Therefore NSFL1C helps maintain protein quality control a critical cellular process that prevents accumulation of dysfunctional proteins.

Pathways

NSFL1C connects closely with the ubiquitin-proteasome pathway and autophagy process. Through its association with p97/VCP NSFL1C facilitates the disassembly and recycling of ubiquitinated proteins highlighting its role in cellular proteostasis. Another key pathway involves its cooperation with complexes involved in membrane trafficking linking NSFL1C to cellular transport mechanisms. These associations enable it to coordinate the removal of defective proteins and contribute to regular cellular operations.

NSFL1C alterations contribute to the pathology of neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and inclusion body myopathy. Mutations in NSFL1C or its partner protein p97/VCP have been implicated in these conditions leading to impaired clearance of misfolded proteins and disrupted cellular functions. An understanding of NSFL1C's role in these disorders highlights the importance of investigating therapeutic strategies targeting protein quality control mechanisms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Reduces the ATPase activity of VCP (By similarity). Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis (By similarity). May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro) (PubMed : 15498563). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (PubMed : 23649807). Also, regulates spindle orientation during mitosis (PubMed : 23649807).

See full target information NSFL1C

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell chemical biology 23:517-28 PubMed27105284

2016

p97 Composition Changes Caused by Allosteric Inhibition Are Suppressed by an On-Target Mechanism that Increases the Enzyme's ATPase Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Nam-Gu Her,Julia I Toth,Chen-Ting Ma,Yang Wei,Khatereh Motamedchaboki,Eduard Sergienko,Matthew D Petroski

Nature structural & molecular biology 19:1093-100 PubMed23042607

2012

DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage.

Applications

Species

Human

Emily J Davis,Christophe Lachaud,Paul Appleton,Thomas J Macartney,Inke Näthke,John Rouse

Nature structural & molecular biology 19:1084-92 PubMed23042605

2012

DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks.

Applications

Species

Human

Anna Mosbech,Ian Gibbs-Seymour,Konstantinos Kagias,Tina Thorslund,Petra Beli,Lou Povlsen,Sofie Vincents Nielsen,Stine Smedegaard,Garry Sedgwick,Claudia Lukas,Rasmus Hartmann-Petersen,Jiri Lukas,Chunaram Choudhary,Roger Pocock,Simon Bekker-Jensen,Niels Mailand

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NSFL1C antibody

Immunoprecipitation - Anti-NSFL1C antibody (AB95963)

Western blot - Anti-NSFL1C antibody (AB95963)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSFL1C antibody (AB95963)

Western blot - Anti-NSFL1C antibody (AB95963)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise