Rabbit Polyclonal NSFL1C antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NSFL1C aa 1-300.
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Publications
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- Cell chemical biology 23:517-282016p97 Composition Changes Caused by Allosteric Inhibition Are Suppressed by an On-Target Mechanism that Increases the Enzyme's ATPase Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNam-Gu Her,Julia I Toth,Chen-Ting Ma,Yang Wei,Khatereh Motamedchaboki,Eduard Sergienko,Matthew D PetroskiPubMed 27105284
- Nature structural & molecular biology 19:1093-1002012DVC1 (C1orf124) recruits the p97 protein segregase to sites of DNA damage.
- Nature structural & molecular biology 19:1084-922012DVC1 (C1orf124) is a DNA damage-targeting p97 adaptor that promotes ubiquitin-dependent responses to replication blocks.Applications:WBReactive species:HumanAnna Mosbech,Ian Gibbs-Seymour,Konstantinos Kagias,Tina Thorslund,Petra Beli,Lou Povlsen,Sofie Vincents Nielsen,Stine Smedegaard,Garry Sedgwick,Claudia Lukas,Rasmus Hartmann-Petersen,Jiri Lukas,Chunaram Choudhary,Roger Pocock,Simon Bekker-Jensen,Niels MailandPubMed 23042605
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NSFL1C aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNZ2
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/250.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Target data
Function
Reduces the ATPase activity of VCP (By similarity). Necessary for the fragmentation of Golgi stacks during mitosis and for VCP-mediated reassembly of Golgi stacks after mitosis (By similarity). May play a role in VCP-mediated formation of transitional endoplasmic reticulum (tER) (By similarity). Inhibits the activity of CTSL (in vitro) (PubMed:15498563). Together with UBXN2B/p37, regulates the centrosomal levels of kinase AURKA/Aurora A during mitotic progression by promoting AURKA removal from centrosomes in prophase (PubMed:23649807). Also, regulates spindle orientation during mitosis (PubMed:23649807).
Alternative names
UBXN2C, NSFL1C, NSFL1 cofactor p47, UBX domain-containing protein 2C, p97 cofactor p47
Recommended products
Rabbit Polyclonal NSFL1C antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NSFL1C aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NSFL1C aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UNZ2
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NSFL1C also known as p97 cofactor p47 is a protein involved in cellular functions related to NSF ATPases. The protein has a mass of approximately 47 kDa. NSFL1C is expressed in various tissues throughout the body where it plays an active role in modulating interactions between cellular structures. This protein mainly functions by regulating membrane fusion events and protein degradation working closely with other components to maintain cellular integrity.
- Biological function summary
NSFL1C acts as an important modulator in cellular homeostasis often participating in complexes with other proteins like p97/VCP a well-known AAA ATPase. This partnership allows NSFL1C to influence diverse cellular activities including endoplasmic-reticulum-associated protein degradation (ERAD) ensuring the removal of misfolded proteins. Therefore NSFL1C helps maintain protein quality control a critical cellular process that prevents accumulation of dysfunctional proteins.
- Pathways
NSFL1C connects closely with the ubiquitin-proteasome pathway and autophagy process. Through its association with p97/VCP NSFL1C facilitates the disassembly and recycling of ubiquitinated proteins highlighting its role in cellular proteostasis. Another key pathway involves its cooperation with complexes involved in membrane trafficking linking NSFL1C to cellular transport mechanisms. These associations enable it to coordinate the removal of defective proteins and contribute to regular cellular operations.
- Associated diseases and disorders
NSFL1C alterations contribute to the pathology of neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and inclusion body myopathy. Mutations in NSFL1C or its partner protein p97/VCP have been implicated in these conditions leading to impaired clearance of misfolded proteins and disrupted cellular functions. An understanding of NSFL1C's role in these disorders highlights the importance of investigating therapeutic strategies targeting protein quality control mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-NSFL1C antibody (ab95963)
All lanes: Western blot - Anti-NSFL1C antibody (ab95963) at 1/1000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Lane 3: H1299 whole cell lysate at 30 µg
Predicted band size: 41 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSFL1C antibody (ab95963)
ab95963, at 1/100, staining paraffin-embedded differential N/T pair Lung SCC cancer by Immunohistochemistry.
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