JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB189513

Anti-NSG2 antibody [EPR15389(B)] - C-terminal

5

(1 Review)

|

(7 Publications)

Rabbit Recombinant Monoclonal NSG2 antibody. C-terminal. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

View Alternative Names

CALY3, NSG2, Neuronal vesicle trafficking-associated protein 2, Neuron-specific protein family member 2, Protein p19, Hmp19

2 Images
Western blot - Anti-NSG2 antibody [EPR15389(B)] - C-terminal (AB189513)
  • WB

Supplier Data

Western blot - Anti-NSG2 antibody [EPR15389(B)] - C-terminal (AB189513)

All lanes:

Western blot - Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513) at 1/1000 dilution

Lane 1:

SH-SY5Y cell lysate at 10 µg

Lane 2:

Rat brain lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 19 kDa

false

Western blot - Anti-NSG2 antibody [EPR15389(B)] - C-terminal (AB189513)
  • WB

Supplier Data

Western blot - Anti-NSG2 antibody [EPR15389(B)] - C-terminal (AB189513)

All lanes:

Western blot - Anti-NSG2 antibody [EPR15389(B)] - C-terminal (ab189513) at 1/1000 dilution

Lane 1:

Neuro-2a cell lysate at 20 µg

Lane 2:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 19 kDa

false

  • Carrier free

    Anti-NSG2 antibody [EPR15389(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15389(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB137029

Anti-RAB7 antibody [EPR7589] - Late Endosome Marker

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-RAB7 antibody [EPR7589] - Late Endosome Marker (AB137029)

  • 5
  • 25
Primary Antibodies

AB124767

Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker

RabMAb|Recombinant|KO Validated|20ul selling size

Western blot - Anti-M6PR (cation independent) antibody [EPR6599] - Lysosome Membrane Marker (AB124767)

  • 5
  • 7
Primary Antibodies

AB157220

Anti-VPS35 antibody [EPR11501(B)]

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-VPS35 antibody [EPR11501(B)] (AB157220)

  • 2
  • 1
Primary Antibodies

AB52866

Anti-alpha Tubulin antibody [EP1332Y] - Loading Control

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-alpha Tubulin antibody [EP1332Y] - Loading Control (AB52866)

  • 5
  • 31
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSG2 also known as Neuronal Vesicle Trafficking Associated 2 is a protein involved in intracellular processes. It has a molecular mass of approximately 49 kDa. NSG2 predominantly expresses in the brain particularly in neurons which suggests its critical role in neural activity. The protein localizes in synaptic vesicles hinting at its involvement in synaptic functions. Its expression is tightly regulated during neuronal development indicating its potential role in neural differentiation and maturation.
Biological function summary

The NSG2 protein contributes to vesicle trafficking within neuronal cells. It plays a part in a multiprotein complex that may regulate synaptic vesicle cycling or neurotransmitter release. The protein influences synaptic structure and function important for neural communication. Researchers have observed its significant impact on maintaining synaptic plasticity suggesting its role in learning and memory processes.

Pathways

NSG2 participates in neural development and synaptic communication pathways. It interacts within the neurotransmitter release cycle specifically affecting the synaptic vesicle cycle. In this context proteins such as SNAP-25 and synaptotagmin may have close interactions with NSG2 through these pathways. These interactions highlight the significance of NSG2 in neural circuit stability and function impacting neuronal transmission efficiency.

NSG2 has associations with neurological conditions including autism spectrum disorder and schizophrenia. Altered expression or mutation of NSG2 links it with these cognitive and developmental disorders. The protein's interactions with other targets like SNAP-25 and synaptotagmin might play a part in the manifestation or progression of these conditions. Understanding NSG2's role in these disorders could contribute to the development of therapeutic strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information NSG2
style
left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular brain 17:95 PubMed39695712

2024

Knockout of AMPA receptor binding protein Neuron-specific gene 2 (NSG2) enhances associative learning and cognitive flexibility.

Applications

Unspecified application

Species

Unspecified reactive species

Amber J Zimmerman,Antonio Serrano-Rodriguez,Melody Sun,Sandy J Wilson,David N Linsenbardt,Jonathan L Brigman,Jason P Weick

The Journal of biological chemistry 299:105446 PubMed37949230

2023

Neuron-specific gene NSG1 binds to and positively regulates sortilin ectodomain shedding via a metalloproteinase-dependent mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Malene Overby,Antonio Serrano-Rodriguez,Somayeh Dadras,Ann Kathrine Christiansen,Gözde Ozcelik,Stefan F Lichtenthaler,Jason Porter Weick,Heidi Kaastrup Müller

Genes, brain, and behavior 21:e12816 PubMed35577358

2022

Global loss of Neuron-specific gene 1 causes alterations in motor coordination, increased anxiety, and diurnal hyperactivity in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Roman Austin,Praveen Chander,Amber J Zimmerman,Malene Overby,Laura Digilio,Chan Choo Yap,David N Linsenbardt,Heidi Kaastrup Müller,Jason P Weick

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:4415-4434 PubMed35474277

2022

Dynein Is Required for Rab7-Dependent Endosome Maturation, Retrograde Dendritic Transport, and Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Chan Choo Yap,Laura Digilio,Lloyd P McMahon,Tuanlao Wang,Bettina Winckler

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada 27:400-408 PubMed33478608

2021

Loss of Arf Guanine Nucleotide Exchange Factor GBF1 Activity Disturbs Organelle Dynamics in Mouse Oocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Jing Zou,Meng-Meng Shan,Zhen-Nan Pan,Meng-Hao Pan,Xiao-Han Li,Shao-Chen Sun

eNeuro 6: PubMed30680309

2019

Neuron-Specific Gene 2 (NSG2) Encodes an AMPA Receptor Interacting Protein That Modulates Excitatory Neurotransmission.

Applications

Unspecified application

Species

Unspecified reactive species

Praveen Chander,Matthew J Kennedy,Bettina Winckler,Jason P Weick

The Journal of comparative neurology 525:1861-1878 PubMed28299779

2017

The related neuronal endosomal proteins NEEP21 (Nsg1) and P19 (Nsg2) have divergent expression profiles in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly Barford,Chan Choo Yap,Noelle D Dwyer,Bettina Winckler
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com