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AB96804

Anti-NSMAF antibody

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Rabbit Polyclonal NSMAF antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NSMAF aa 250-550.

View Alternative Names

FAN, NSMAF, Protein FAN, Factor associated with neutral sphingomyelinase activation, Factor associated with N-SMase activation

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMAF antibody (AB96804)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSMAF antibody (AB96804)

ab96804, at a 1/100 dilution, staining NSMAF in paraffin-embedded T(gastric CA) by Immunohistochemical analysis.

Western blot - Anti-NSMAF antibody (AB96804)
  • WB

Unknown

Western blot - Anti-NSMAF antibody (AB96804)

7.5% SDS PAGE

All lanes:

Western blot - Anti-NSMAF antibody (ab96804) at 1/500 dilution

Lane 1:

Non-transfected 293T cell lysate

Lane 2:

NSMAF transfected 293T cell lysate

Predicted band size: 104 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NSMAF aa 250-550. The exact immunogen used to generate this antibody is proprietary information.

Q92636

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSMAF also known as Neutral Sphingomyelinase Activation Associated Factor has a mass of approximately 61 kDa. It plays a central mechanical role in cellular stress responses by interacting with neutral sphingomyelinase enzymes. NSMAF expression occurs in a variety of tissues including heart liver and lung. The protein directly influences the activity of sphingomyelinase facilitating the conversion of sphingomyelin to ceramide a bioactive lipid linked to cellular signaling.
Biological function summary

NSMAF influences cellular apoptosis and proliferation through its involvement in the sphingolipid signaling pathway. It forms part of a protein complex that contributes to ceramide-mediated signaling pathways. NSMAF's interactions extend to various cellular processes including inflammation and immune responses positioning it as an important factor in cell fate determination. The impact on ceramide production reinforces the protein's role in modulating stress-activated responses within cells.

Pathways

NSMAF participates in the sphingolipid metabolism pathway and is also integrated into the TNF signaling pathway. Its influence in the sphingolipid metabolism pathway affects ceramide levels influencing cellular apoptosis. Additionally NSMAF interacts with TNF-related proteins emphasizing its role in mediating responses triggered by inflammatory cytokines like tumor necrosis factor. These pathways highlight NSMAF's involvement in regulating cellular stress responses via ceramide production and apoptosis.

NSMAF has ties to cancer and neurodegenerative diseases. Aberrant NSMAF function or expression can lead to misregulated apoptotic processes contributing to tumor progression or cell death in neurodegenerative conditions. Its interaction with the Fas-associated protein death domain (FADD) protein links it to apoptotic pathways malfunctioning in these disorders. Understanding NSMAF provides valuable insights into its involvement in these disease mechanisms highlighting potential therapeutic targets for regulating apoptosis and inflammation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Couples the p55 TNF-receptor (TNF-R55 / TNFR1) to neutral sphingomyelinase (N-SMASE). Specifically binds to the N-smase activation domain of TNF-R55. May regulate ceramide production by N-SMASE.
See full target information NSMAF
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Product promise

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