JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB238568

Anti-nSMase antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal nSMase antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Sphingomyelin phosphodiesterase 2 aa 150-350.

View Alternative Names

Sphingomyelin phosphodiesterase 2, Lyso-platelet-activating factor-phospholipase C, Neutral sphingomyelinase, Lyso-PAF-PLC, N-SMase, nSMase, nSMase1, SMPD2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nSMase antibody (AB238568)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nSMase antibody (AB238568)

Paraffin-embedded human pancreas tissue stained for nSMase using ab238568 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nSMase antibody (AB238568)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nSMase antibody (AB238568)

Paraffin-embedded human endometrial cancer tissue stained for nSMase using ab238568 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-nSMase antibody (AB238568)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-nSMase antibody (AB238568)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for nSMase (green) using ab238568 at 1/66 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L) secondary antibody.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Sphingomyelin phosphodiesterase 2 aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

O60906

style
left-column

Alternative Products

Primary Antibodies

AB131330

Anti-nSMase antibody [EPR6718]

RabMAb|Recombinant

Western blot - Anti-nSMase antibody [EPR6718] (AB131330)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB85017

Anti-NSMase2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-NSMase2 antibody (AB85017)

  • 4
  • 2
Primary Antibodies

AB235057

Anti-Sphingomyelin Synthase 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sphingomyelin Synthase 1 antibody (AB235057)

  • 0
  • 0
Primary Antibodies

AB21685

Anti-GRP78 BiP antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRP78 BiP antibody (AB21685)

  • 5
  • 71
Primary Antibodies

AB185141

Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-GSK3 beta + GSK3 alpha antibody [EPR18814-102] (AB185141)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSMase also known as neutral sphingomyelinase is an enzyme with a molecular mass typically ranging between 43 to 48 kDa. It plays an important role in the hydrolysis of sphingomyelin to generate ceramide and phosphocholine. nSMase is distributed widely in various tissues with higher expression levels observed in the brain liver and spleen. This enzyme exists in multiple isoforms each localized in different cellular compartments contributing to diverse cellular functions.
Biological function summary

Neutral sphingomyelinase influences several cellular processes by modulating ceramide levels. Ceramide a bioactive lipid serves as a secondary messenger in signal transduction pathways and mediates processes like apoptosis and cell differentiation. nSMase often functions as a monomer but it can also be part of larger complexes with other proteins to execute its roles in cellular signaling. Through its enzymatic activity nSMase impacts membrane structure and dynamics affecting overall cellular homeostasis.

Pathways

Neutral sphingomyelinase is involved in key signaling pathways like the MAPK and apoptosis pathways. In the MAPK pathway nSMase-derived ceramide acts as a signaling molecule that can activate kinases involved in stress responses. Additionally its role in apoptosis links it to various stress signals where it facilitates the programmed cell death process. Interaction with proteins such as caspase and protein kinase C highlights its integration into signaling networks illustrating its regulatory significance.

Altered nSMase activity associates with cancers and neurodegenerative diseases. In cancer changes in ceramide production by nSMase can impact cell proliferation and survival connecting it with oncogenic processes. In neurodegenerative disorders such as Alzheimer's disease dysregulated nSMase activity influences neuroinflammation and neuronal cell death. It's interaction with amyloid precursor protein in such conditions highlights the enzyme's potential as a therapeutic target for modulating disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Catalyzes, at least in vitro, the hydrolysis of sphingomyelin to form ceramide and phosphocholine (PubMed : 10608884). Also hydrolyzes 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor) in vivo (PubMed : 10608884). Also acts on 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and sphingosylphosphocholine (PubMed : 10608884, PubMed : 14741383).
See full target information Sphingomyelin phosphodiesterase 2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity, inflammation and disease 11:e1022 PubMed37904707

2023

The CD2-CD58 axis: A novel marker predicting poor prognosis in patients with low-grade gliomas and potential therapeutic approaches.

Applications

Unspecified application

Species

Unspecified reactive species

Mingwei Wu,Yiyuan Chen,Gao Hua,Liu Chunhui
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com