AB131330

Anti-nSMase antibody [EPR6718]

RabMAb
Recombinant
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(6 Publications)

Rabbit Recombinant Monoclonal nSMase antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 6 publications.

View Alternative Names

Sphingomyelin phosphodiesterase 2, Lyso-platelet-activating factor-phospholipase C, Neutral sphingomyelinase, Lyso-PAF-PLC, N-SMase, nSMase, nSMase1, SMPD2

5 Images

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Immunoprecipitation - Anti-nSMase antibody [EPR6718] (AB131330)

ab131330 (purified) at 1 : 30 dilution (2μg) immunoprecipitating nSMase in Mouse brain lysate.
Lane 1 (input) : Mouse brain lysate 10μg
Lane 2 (+) : ab131330 & Mouse brain lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab131330 in Mouse brain lysate
For western blotting, VeriBlot for IP Detection Reagent(HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-nSMase antibody [EPR6718] (ab131330)

Predicted band size: 48 kDa

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Western blot - Anti-nSMase antibody [EPR6718] (AB131330)

This image was generated using the unpurified version of the product.

All lanes:

Western blot - Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

MOLT4 cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 48 kDa

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Western blot - Anti-nSMase antibody [EPR6718] (AB131330)

This image was generated using the unpurified version of the product.

All lanes:

Western blot - Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution

Lane 1:

Mouse brain lysate at 10 µg

Lane 2:

Rat brain lysates at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 48 kDa

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Lab

Western blot - Anti-nSMase antibody [EPR6718] (AB131330)

All lanes:

Western blot - Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution

Lane 1:

MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates at 20 µg

Lane 2:

Mouse brain lysates at 20 µg

Lane 3:

Rat brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 48 kDa

Observed band size: 48 kDa

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OI-RD Scanning - Anti-nSMase antibody [EPR6718] (AB131330)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-nSMase antibody [EPR6718] (AB131330)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6718

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NSMase also known as neutral sphingomyelinase is an enzyme with a molecular mass typically ranging between 43 to 48 kDa. It plays an important role in the hydrolysis of sphingomyelin to generate ceramide and phosphocholine. nSMase is distributed widely in various tissues with higher expression levels observed in the brain liver and spleen. This enzyme exists in multiple isoforms each localized in different cellular compartments contributing to diverse cellular functions.

Biological function summary

Neutral sphingomyelinase influences several cellular processes by modulating ceramide levels. Ceramide a bioactive lipid serves as a secondary messenger in signal transduction pathways and mediates processes like apoptosis and cell differentiation. nSMase often functions as a monomer but it can also be part of larger complexes with other proteins to execute its roles in cellular signaling. Through its enzymatic activity nSMase impacts membrane structure and dynamics affecting overall cellular homeostasis.

Pathways

Neutral sphingomyelinase is involved in key signaling pathways like the MAPK and apoptosis pathways. In the MAPK pathway nSMase-derived ceramide acts as a signaling molecule that can activate kinases involved in stress responses. Additionally its role in apoptosis links it to various stress signals where it facilitates the programmed cell death process. Interaction with proteins such as caspase and protein kinase C highlights its integration into signaling networks illustrating its regulatory significance.

Altered nSMase activity associates with cancers and neurodegenerative diseases. In cancer changes in ceramide production by nSMase can impact cell proliferation and survival connecting it with oncogenic processes. In neurodegenerative disorders such as Alzheimer's disease dysregulated nSMase activity influences neuroinflammation and neuronal cell death. It's interaction with amyloid precursor protein in such conditions highlights the enzyme's potential as a therapeutic target for modulating disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes, at least in vitro, the hydrolysis of sphingomyelin to form ceramide and phosphocholine (PubMed : 10608884). Also hydrolyzes 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor) in vivo (PubMed : 10608884). Also acts on 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and sphingosylphosphocholine (PubMed : 10608884, PubMed : 14741383).

See full target information Sphingomyelin phosphodiesterase 2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of lipid research 65:100559 PubMed38729351

2024

Sphingomyelin is involved in regulating UCP1-mediated nonshivering thermogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Detian Hu,Houyu Zhang,Zhen Liu,Carlos F Ibáñez,Cai Tie,Meng Xie

Oncogenesis 11:67 PubMed36333295

2022

Ablation of sphingosine kinase 2 suppresses fatty liver-associated hepatocellular carcinoma via downregulation of ceramide transfer protein.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Tracy Liu,Long Hoa Chung,Da Liu,Jinbiao Chen,Yu Huang,Jonathan D Teo,Xingxing Daisy Han,Yinan Zhao,Fiona H X Guan,Collin Tran,Jun Yup Lee,Timothy A Couttas,Ken Liu,Geoffery W McCaughan,Mark D Gorrell,Anthony S Don,Shubiao Zhang,Yanfei Qi

Cancers 12: PubMed33050528

2020

Sphingolipid Pathway as a Source of Vulnerability in IDH1 Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Tyrone Dowdy,Lumin Zhang,Orieta Celiku,Sriya Movva,Adrian Lita,Victor Ruiz-Rodado,Mark R Gilbert,Mioara Larion

International journal of molecular sciences 20: PubMed31766565

2019

In Human and Mouse Spino-Cerebellar Tissue, Ataxin-2 Expansion Affects Ceramide-Sphingomyelin Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Nesli-Ece Sen,Aleksandar Arsovic,David Meierhofer,Susanne Brodesser,Carola Oberschmidt,Júlia Canet-Pons,Zeynep-Ece Kaya,Melanie-Vanessa Halbach,Suzana Gispert,Konrad Sandhoff,Georg Auburger

International journal of molecular sciences 20: PubMed31574943

2019

Changes in Membrane Ceramide Pools in Rat Soleus Muscle in Response to Short-Term Disuse.

Applications

Unspecified application

Species

Unspecified reactive species

Alexey M Petrov,Maria N Shalagina,Vladimir A Protopopov,Valeriy G Sergeev,Sergey V Ovechkin,Natalia G Ovchinina,Alexey V Sekunov,Andrey L Zefirov,Guzalia F Zakirjanova,Irina G Bryndina

Biogerontology 16:747-59 PubMed26296420

2015

Muscle-specificity of age-related changes in markers of autophagy and sphingolipid metabolism.

Applications

Species

Rat

David W Russ,Iva M Boyd,Katherine M McCoy,Katherine W McCorkle

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-nSMase antibody [EPR6718]

Immunoprecipitation - Anti-nSMase antibody [EPR6718] (AB131330)

Western blot - Anti-nSMase antibody [EPR6718] (AB131330)

Western blot - Anti-nSMase antibody [EPR6718] (AB131330)

Western blot - Anti-nSMase antibody [EPR6718] (AB131330)

OI-RD Scanning - Anti-nSMase antibody [EPR6718] (AB131330)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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