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Rabbit Recombinant Monoclonal nSMase antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

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Images

Immunoprecipitation - Anti-nSMase antibody [EPR6718] (AB131330), expandable thumbnail
  • Western blot - Anti-nSMase antibody [EPR6718] (AB131330), expandable thumbnail
  • Western blot - Anti-nSMase antibody [EPR6718] (AB131330), expandable thumbnail
  • Western blot - Anti-nSMase antibody [EPR6718] (AB131330), expandable thumbnail
  • OI-RD Scanning - Anti-nSMase antibody [EPR6718] (AB131330), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-P
Human
Expected
Not recommended
Tested
Not recommended
Mouse
Tested
Not recommended
Tested
Not recommended
Rat
Expected
Not recommended
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/10 - 1/100
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Catalyzes, at least in vitro, the hydrolysis of sphingomyelin to form ceramide and phosphocholine (PubMed:10608884). Also hydrolyzes 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor) in vivo (PubMed:10608884). Also acts on 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and sphingosylphosphocholine (PubMed:10608884, PubMed:14741383).

Alternative names

Sphingomyelin phosphodiesterase 2, Lyso-platelet-activating factor-phospholipase C, Neutral sphingomyelinase, Lyso-PAF-PLC, N-SMase, nSMase, nSMase1, SMPD2

Recommended products

Rabbit Recombinant Monoclonal nSMase antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR6718
Purification technique
Affinity purification Protein A
Dissociation constant
6.2 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

NSMase also known as neutral sphingomyelinase is an enzyme with a molecular mass typically ranging between 43 to 48 kDa. It plays an important role in the hydrolysis of sphingomyelin to generate ceramide and phosphocholine. nSMase is distributed widely in various tissues with higher expression levels observed in the brain liver and spleen. This enzyme exists in multiple isoforms each localized in different cellular compartments contributing to diverse cellular functions.

Biological function summary

Neutral sphingomyelinase influences several cellular processes by modulating ceramide levels. Ceramide a bioactive lipid serves as a secondary messenger in signal transduction pathways and mediates processes like apoptosis and cell differentiation. nSMase often functions as a monomer but it can also be part of larger complexes with other proteins to execute its roles in cellular signaling. Through its enzymatic activity nSMase impacts membrane structure and dynamics affecting overall cellular homeostasis.

Pathways

Neutral sphingomyelinase is involved in key signaling pathways like the MAPK and apoptosis pathways. In the MAPK pathway nSMase-derived ceramide acts as a signaling molecule that can activate kinases involved in stress responses. Additionally its role in apoptosis links it to various stress signals where it facilitates the programmed cell death process. Interaction with proteins such as caspase and protein kinase C highlights its integration into signaling networks illustrating its regulatory significance.

Associated diseases and disorders

Altered nSMase activity associates with cancers and neurodegenerative diseases. In cancer changes in ceramide production by nSMase can impact cell proliferation and survival connecting it with oncogenic processes. In neurodegenerative disorders such as Alzheimer's disease dysregulated nSMase activity influences neuroinflammation and neuronal cell death. It's interaction with amyloid precursor protein in such conditions highlights the enzyme's potential as a therapeutic target for modulating disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-nSMase antibody [EPR6718] (ab131330), expandable thumbnail

    Immunoprecipitation - Anti-nSMase antibody [EPR6718] (ab131330)

    ab131330 (purified) at 1:30 dilution (2μg) immunoprecipitating nSMase in Mouse brain lysate.
    Lane 1 (input): Mouse brain lysate 10μg
    Lane 2 (+): ab131330 & Mouse brain lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab131330 in Mouse brain lysate
    For western blotting, VeriBlot for IP Detection Reagent(HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-nSMase antibody [EPR6718] (ab131330)

    Predicted band size: 48 kDa

  • Western blot - Anti-nSMase antibody [EPR6718] (ab131330), expandable thumbnail

    Western blot - Anti-nSMase antibody [EPR6718] (ab131330)

    All lanes: Western blot - Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution

    Lane 1: MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates at 20 µg

    Lane 2: Mouse brain lysates at 20 µg

    Lane 3: Rat brain lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 48 kDa

    Observed band size: 48 kDa

  • Western blot - Anti-nSMase antibody [EPR6718] (ab131330), expandable thumbnail

    Western blot - Anti-nSMase antibody [EPR6718] (ab131330)

    This image was generated using the unpurified version of the product.

    All lanes: Western blot - Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution

    Lane 1: 293T cell lysate at 10 µg

    Lane 2: MOLT4 cell lysate at 10 µg

    Lane 3: K562 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 48 kDa

  • Western blot - Anti-nSMase antibody [EPR6718] (ab131330), expandable thumbnail

    Western blot - Anti-nSMase antibody [EPR6718] (ab131330)

    This image was generated using the unpurified version of the product.

    All lanes: Western blot - Anti-nSMase antibody [EPR6718] (ab131330) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Rat brain lysates at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 48 kDa

  • OI-RD Scanning - Anti-nSMase antibody [EPR6718] (ab131330), expandable thumbnail

    OI-RD Scanning - Anti-nSMase antibody [EPR6718] (ab131330)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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